Characterization of the DNA-binding domain of β protein, a component of phage λ Red-pathway, by UV catalyzed cross-linking

Gene

Volumn 182, Issue 1-2, 1996, Pages 81-87

  Mythili, E ^a   Anand Kumar, K ^a   Muniyappa, K ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

Circular dichroism; DNA protein interaction; Genetic recombination; Nucleoprotein complexes; Stoichiometry

Indexed keywords

BACTERIAL PROTEIN; CHYMOTRYPSIN; DNA BINDING PROTEIN; GLUTARALDEHYDE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIOPHAGE LAMBDA; CONTROLLED STUDY; DISSOCIATION CONSTANT; ELECTROPHORETIC MOBILITY; ESCHERICHIA COLI; GENETIC RECOMBINATION; NONHUMAN; PHOTOCHEMISTRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN PURIFICATION; STOICHIOMETRY; STRUCTURE ACTIVITY RELATION; ULTRAVIOLET IRRADIATION;

BACTERIOPHAGE LAMBDA; CHYMOTRYPSIN; CIRCULAR DICHROISM; CROSS-LINKING REAGENTS; DIMERIZATION; DNA, SINGLE-STRANDED; DNA-BINDING PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GLUTARAL; NUCLEOPROTEINS; OSMOLAR CONCENTRATION; PROTEIN CONFORMATION; RECOMBINATION, GENETIC; SODIUM CHLORIDE; ULTRAVIOLET RAYS; VIRAL PROTEINS;

BACTERIA (MICROORGANISMS); BACTERIOPHAGE LAMBDA; ESCHERICHIA COLI;

EID: 0030571482     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00518-5     Document Type: Article

References (30)

1. Carter, D.M. and Radding, C.M. (1971) The role of exonuclease and β protein of phage λ. in genetic recombination, II. Substrate specificity and the mode of action of λ exonuclease. J. Biol. Chem. 246, 2502-2512.
2. Cassuto, E. and Radding, C.M. (1971) A mechanism for the action of λ exonuclease in genetic recombination. Nature (London) New Biol. 229, 13-16.
3. Clark, A.J. and Sandler, S.J. (1994) Homologous genetic recombination: The pieces begin to fall into place. Crit. Rev. Microbiol. 20, 125-142.
4. Haber, J.E. (1995) In vivo biochemistry: physical monitoring of recombination induced by site-specific endonuclease. BioEssays 17, 609-620.
5. Hall, S.D., Kane, M.F. and Kolodner, R.D. (1993) Identification and characterization of the Escherichia coli RecT protein, a protein encoded by the recE region that promotes renaturation of homologous single-stranded DNA. J. Bacteriol. 175, 277-287.
6. Hoess, R., Abremski, K., Irwin, S., Kendall, M. and Mack, A. (1990) DNA specificity of the Cre recombinase resides in the 25 kDa carboxyl domain of the protein. J. Mol. Biol. 216, 873-882.
7. Johnson, C.W., Jr. (1990) Protein secondary structure and circular dichroism: a practical guide. Proteins: Struct. Function Genet. 7, 205-214.
8. Joseph, J.W. and Kolodner, R.D. (1983) Exonuclease VIII of Escherichia coli. I. Purification and physical properties. J. Biol. Chem. 258, 10411-10424.
9. Kmiec, E.B. and Holloman, W. (1981) β protein of bactenophage λ promotes renaturation of DNA. J. Biol. Chem. 256, 12636-12639.
10. Kolodner, R.D., Hall, S.D. and Luisi-DeLuca, C. (1994) Homologous pairing proteins encoded by the Escherichia coli recE and recTgenes. Mol. Microbiol. 11, 23-30.
11. Kowalczykowski, S.K., Dixon, D.A., Eggleston, A.K., Lauder, S.D. and Rehrauer, W.M. (1994) Biochemistry of homologous recombination in Escherichia coli. Microbiol. Rev. 58, 991-1043.
12. Kumar, A.K., Mahalakshmi, S. and Muniyappa, K. (1993) DNA-induced conformational changes in RecA protein: evidence for structural heterogeneity among nucleoprotein filaments and implications for homologous pairing. J. Biol. Chem. 268, 26162-26170.
13. Kusano, K., Sunohara, Y., Takahashi, N., Yoshikura, H. and Kobayashi, I. (1994) DNA double-strand break repair: Genetic determinants of flanking crossing-over. Proc. Natl. Acad. Sci. USA 91, 1173-1177.
14. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680-685
15. Leahy, M. and Radding, C.M. (1986) Topography of the interaction of RecA protein with single-stranded deoxynucleotides. J. Biol. Chem. 261, 6954-6960.
16. Little, J.W. (1967) An exonuclease induced by bacteriophage λ. II. Nature of the enzymic reaction J. Biol. Chem. 242, 679-686.
17. Luisi-DeLuca, C., Lovett, S.T. and Kolodner, R.D. (1989) Genetic and physical analysis of plasmid recombination in recB recC sbcB and recB recC shcA Escherichia coli K-12 mutants. Genetics 122, 269-278.
18. Muniyappa, K. and Radding, C.M. (1986) The homologous recombination system of bacteriophage λ: pairing activities of β protein. J. Biol. Chem. 261, 7472-7478.
19. Muniyappa, K. and Mythili, E. (1993) Phage λ β protein, a component of general genetic recombination, is associated with host ribosomal protein S1. Biochem. and Mol. Biol. Int. 31, 1-11.
20. Nussbaum, A., Shalit, M. and Cohen, A. (1992) Restriction-stimulated homologous recombination of plasmids by the RecE pathway of Escherichia coli. Genetics 130, 37-49.
21. Poteete, A.R. and Fenton, A.C. (1993) Efficient double-strand stimulated recombination promoted by the general recombination systems of phages λ and P22. Genetics 134, 1013-1021.
22. Silberstein, Z., Tzfati, Y. and Cohen, A. (1995) Primary products of break-induced recombination by Escherichia coli RecE pathway. J. Bacteriol. 177, 1692-1698.
23. Smith, G.R. (1988) Homologous recombination in prokaryotes. Microbiol. Rev. 52, 1-28.
24. Stahl, F.W., McMilin, K. and Stahl, M.M. (1974) The distribution of crossovers along unreplicated phage λ chromosomes. Genetics 77, 395-408.
25. Stahl, F.W., Kobayashi, I. and Stahl, M.M. (1985) In phage λ, cos is a recombinator in the Red pathway. J. Mol. Biol. 181, 199-209.
26. Takahashi, N. and Kobayashi, I. (1990) Evidence for the double-strand break repair model of bacteriophage λ recombination. Proc. Natl. Acad. Sci. USA. 87, 2790-2794.
27. Takahashi, N., Kusano, K., Yokochi, Y., Kitamura, Y., Yoshikura, H. and Kobayashi, I. (1993) Genetic analysis of double-strand break repair in Escherichia coli. J. Bacteriol. 175, 5176-5185.
28. Thaler, D. and Stahl, F.W. (1988) DNA double-chain breaks in recombination of phage λ and of yeast. Annu. Rev. Genet. 22, 169-192.
29. Williams, K.R. and Konigsberg, W.H. (1991) Identification of amino acid residues at interface of protein-nucleic acid complexes by photochemical cross-linking. Methods Enzymol. 208, 516-539.
30. Yokochi, T., Kusano, K. and Kobayashi, I. (1995) Evidence for conservative (two-progeny) DNA double-strand break repair. Genetics 139, 5-17.