Altering the specificity of subtilisin B. lentus by combining site-directed mutagenesis and chemical modification

Bioorganic and Medicinal Chemistry Letters

Volumn 6, Issue 21, 1996, Pages 2507-2512

  Berglund, Per ^a   Stabile, Michele R ^a   Gold, Marvin ^b   Jones, J Bryan ^a   Mitchinson, Colin ^c   Bott, Richard R ^c   Graycar, Thomas P ^c  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c GENENCOR INTERNATIONAL INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BORONIC ACID DERIVATIVE; SUBTILISIN;

ARTICLE; EGGERTHELLA LENTA; ENZYME ACTIVITY; ENZYME MODIFICATION; SITE DIRECTED MUTAGENESIS;

BACILLUS LENTUS; EGGERTHELLA LENTA;

EID: 0030571292     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0960-894X(96)00467-2     Document Type: Article

References (29)

1. 1. Cornish, V. W.; Mendel, D.; Schultz, P. G. Angew. Chem., Int. Ed. Engl. 1995, 34, 621.
2. 2. Polgar, L.; Bender, M. L. J. Am. Chem. Soc. 1966, 88, 3153.
3. 3. Neet, K. E.; Koshland, D. E., Jr. Proc. Natl. Acad. Sci. USA 1966, 56, 1606.
4. 4. (a) Hilvert, D.; Kaiser, E. T. J. Am. Chem. Soc. 1985, 107, 5805.
5. (b) Radziejewski, C.; Ballou, D. P.; Kaiser, E. T. J. Am. Chem. Soc. 1985, 107, 3352.
6. (c) Stewart, K. D.; Radziejewski, C.; Kaiser, E. T. J. Am. Chem. Soc. 1986, 108, 3480.
7. (d) Rokita, S. E.; Kaiser, E. T. J. Am. Chem. Soc. 1986, 108, 4984.
8. (e) Kokubo, T.; Sassa, S.; Kaiser, E. T. J. Am. Chem. Soc. 1987, 109, 606.
9. (f) Kaiser, E. T. Angew. Chem., Int. Ed. Engl. 1988, 27, 913.
10. (g) Hilvert, D.; Hatanaka, Y.; Kaiser, E. T. J. Am. Chem. Soc. 1988, 110, 682.
11. 5. (a) Wu, Z.-P.; Hilvert, D. J. Am. Chem. Soc. 1989, 111, 4514.
12. (b) Bell, I. M.; Fisher, M. L.; Wu, Z.-P., Hilvert, D. Biochemistry, 1993, 32, 3754.
13. (c) Peterson, E. B.; Hilvert, D. Biochemistry 1995, 34, 6616.
14. 6. Suckling, C. J.; Zhu, L.-M. Bioorg. Med. Chem. Lett. 1993, 3, 531.
15. 7. (a) Smith, D. J.; Kenyon, G. L. J. Biol. Chem. 1974, 249, 3317.
16. (b) Kenyon, G. L.; Bruice, T. W. Methods Enzymol. 1977, 47, 407.
17. (c) Brocklehurst, K. Int. J. Biochem, 1979, 10, 259.
18. (d) Bruice, T. W.; Kenyon, G. L. J. Protein Chem. 1982, 1, 47.
19. (e) Wynn R.; Richards, F. M. Methods Enzymol. 1995, 257, 351.
20. 8. Bech, L. M.; Breddam, K. Carlsberg Res. Commun. 1988, 53, 381.
21. 9. Wynn, R.; Harkins, P. C.; Richards, F. M.; Fox, R. O. Protein Sci. 1996, 5, 1026.
22. 10. Grøn, H.; Bech, L. M.; Branner, S.; Breddam, K. Eur. J. Biochemistry 1990, 194, 897.
23. 11. Compounds 2 and 3 were purchased from Toronto Research Chemicals, 4481 Chesswood Dr., Downsview, Ontario, Canada M3J 2C3.
24. 12. Stabile, M. R.; Lai, W. G.; DeSantis, G.; Gold, M.; Jones, J. B.; Mitchinson, C.; Bott, R. R.; Graycar, T. P.; Liu, C.-C. Bioorg. Med. Chem. Lett. 1996, in press.
25. + calcd: 26746; found: 26744.
26. 14. Cleland, W. W. Biochemistry 1964, 3, 480.
27. 15. Graycar, T. P.; Bott, R. R.; Caldwell, R. M.; Dauberman, J. L.; Lad, P. J.; Power, S. D.; Sagar, I. H.; Silva, R. A.; Weiss, G. L.; Woodhouse, L. R.; Estell, D. A. Ann. N. Y. Acad. Sci. 1992, 672, 71.
28. 16. Hsia, C. Y.; Ganshaw, G.; Paech, C.; Murray, C. J. Anal. Biochem. 1996, in press.
29. 17. Waley, S. G. Biochem. J. 1982, 205, 631.