Probing the specificity of the S1 binding site of M222 mutants of subtilisin B. lentus with boronic acid inhibitors

Bioorganic and Medicinal Chemistry Letters

Volumn 6, Issue 21, 1996, Pages 2501-2506

  Stabile, Michele R ^a   Lai, W George ^a   DeSantis, Grace ^a   Gold, Marvin ^b   Jones, J Bryan ^a   Mitchinson, Colin ^c   Bott, Richard R ^c   Graycar, Thomas P ^c   Liu, Chung Cheng ^c  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c GENENCOR INTERNATIONAL INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BORONIC ACID DERIVATIVE; SUBTILISIN;

ARTICLE; BINDING SITE; EGGERTHELLA LENTA; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME INHIBITION; MUTANT;

EGGERTHELLA LENTA;

EID: 0030571282     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0960-894X(96)00466-0     Document Type: Article

References (18)

1. 1. (a) Preparative Biotransformations; Roberts, S. M., Ed.; Wiley: New York, 1993.
2. (b) Wong, C.-H.; Whitesides, G. M. Enzymes in Synthetic Organic Chemistry; Pergamon: Oxford, 1994.
3. (c) Faber, K. Biotransformations in Organic Chemistry; 2nd ed.; Springer-Verlag: Heidelberg, 1995.
4. 2. (a) Wolfenden, R. Acc. Chem. Res. 1972, 5, 10.
5. (b) O'Connor Westerik, J.; Wolfenden, R. J. Biol. Chem. 1972, 247, 8195.
6. (c) Lienhard, G. E. Science 1973, 180, 149.
7. (d) Thompson, R. C. Biochemistry 1973, 12, 47.
8. (e) Rawn, J. D.; Lienhard, G. E. Biochemistry 1974, 13, 3124.
9. (f) Westmark, P. R.; Kelly, J. P.; Smith, B. D. J. Am. Chem. Soc. 1993, 115, 3416.
10. 3. Seufer-Wasserthal, P.; Martichonok, V.; Keller, T. H.; Chin, B.; Martin, R.; Jones, J. B. Bioorg. Med. Chem. 1994, 2, 35.
11. 4. (a) US Patent 5,185,258.
12. (b) International Patent WO 95/10615.
13. 5. Zoller, M. J.; Smith, M. Methods Enzymol. 1983, 700, 468.
14. 6. Bradford, M. M. Anal. Biochem. 1976, 72, 248.
15. 7. Hsia, C. Y.; Ganshaw, G.; Paech, C.; Murray, C. J. Anal. Biochem. 1996, in press.
16. 8. Waley, S. G. Biochem. J. 1982, 205, 631.
17. 9. Gran, H.; Meldal, M.; Breddam, K. Biochemistry 1992, 31, 6011.
18. 10. Cabani, S.; Gianni, P; Mollica, V.; Lepori, L. J. Solut. Chem. 1981, 10, 563.