Concentration and extinction coefficient determination for oligonucleotides and analogs using a general phosphate analysis

Analytical Biochemistry

Volumn 240, Issue 2, 1996, Pages 273-282

  Murphy, James H ^a,b   Trapane, Tina L ^a  

^a JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

^b PALL CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MOLYBDENUM COMPOUNDS; NUCLEIC ACIDS; OLIGOMERS; OLIGONUCLEOTIDES;

COMPLEX FORMATIONS; EXTINCTION COEFFICIENTS; HIGH TEMPERATURE; NATURALLY OCCURRING; NUCLEIC ACID OLIGOMERS; PHOSPHODIESTERS; PHOSPHOMOLYBDATES; PREDICTIVE METHODS;

COLORIMETRIC ANALYSIS;

OLIGONUCLEOTIDE; PHOSPHATE;

ABSORPTION SPECTROPHOTOMETRY; ANALYTICAL ERROR; ARTICLE; CHEMICAL ANALYSIS; COLORIMETRY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; STOICHIOMETRY;

EID: 0030571001     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1996.0357     Document Type: Article

References (21)

1. 1. Cantor, C. R., Warshaw, M. M., and Shapiro, H. (1970) Biopolymers 9, 1059-1077.
2. 2. Puglisi, J. D., and Tinoco, I. (1989) Methods Enzymol. 180, 304-325.
3. 3. Potter, B. V. L., Connolly, B. A., and Eckstein, F. (1983) Biochemistry 22, 1369-1377.
4. 4. Bryant, F. R., and Benkovic, S. J. (1979) Biochemistry 18, 2825-2828.
5. 5. Wilk, A., and Stec, W. J. (1995) Nucleic Acids Res. 23, 530-534.
6. 6. Baginski, E. S., Foa, P. P., Weiner, L. M., and Zak, B. (1968) Microchem. J. 13, 115-119.
7. 7. Kirkpatrick, D. S., and Bishop, S. H. (1971) Anal. Chem. 43, 1707-1709.
8. 8. Fiske, C. H., and SubbaRow, Y. (1925) J. Biol. Chem. 66, 375-400.
9. 9. Chen, P. S., Toribara, T. Y., and Warner, H. (1956) Anal. Chem. 28, 1756-1758.
10. 10. Miller, P. S., Cushman, C. D., and Levis, J. T., (1991) in Oligonucleotides and Analogues: A Practical Approach (Eckstein, F., Ed.), pp. 137-154, Oxford Univ. Press, Oxford.
11. 11. Beaven, G. H., Holiday, E. R., and Johnson, E. A. (1955) in The Nucleic Acids: Chemistry and Biology (Chargaff, E., and Davidson, J. N., Eds.), Vol. I, pp. 493-553, Academic Press, New York.
12. 12. Murray, A. W., and Atkinson, M. R. (1968) Biochemistry 7, 4023-4029.
13. 13. Antao, V. P., Gray, D. M., and Ratliff, R. L. (1988) Nucleic Acids Res. 16, 719-738.
14. 14. Rippe, K., Fritsch, V., Westhof, E., and Jovin, T. M. (1992) EMBO J. 11, 3777-3786.
15. 15. Fox, J. J., Wempen, I., Hampton, A., and Doerr, I. L. (1958) J. Am. Chem. Soc. 80, 1669-1675.
16. 16. Dunn, D. B., and Hall, R. H. (1975) in Handbook of Biochemistry and Molecular Biology, Nucleic Acids (Fasman, G. D., Ed.), Vol. I, p. 178, CRC Press, Cleveland.
17. 17. Ono, A., Ts'o, P. O. P., and Kan, L.-s. (1992) J. Org. Chem. 57, 3225-3230.
18. 18. Brown, D. M., Todd, A. R., and Varadarajan, S. (1957) J. Chem. Soc., 868-872.
19. 19. Wells, R. D., Larson, J. E., Grant, R. C., Shortle, B. E., and Cantor, C. R. (1970) J. Mol. Biol. 54, 465-497.
20. 20. Howard, F. B., Frazier, J., and Miles, H. T. (1971) J. Biol. Chem. 246, 7073-7086.
21. 21. Webb, M. R. (1992) Proc. Natl. Acad. Sci. USA 89, 4884-4887.