Reconstitution of apo-glucose oxidase with a nitrospiropyran-modified FAD cofactor yields a photoswitchable biocatalyst for amperometric transduction of recorded optical signals

Journal of the American Chemical Society

Volumn 118, Issue 22, 1996, Pages 5310-5311

  Willner, Itamar ^a   Blonder, Ron ^a   Katz, Eugenii ^a   Stocker, Achim ^b   Bückmann, Andreas F ^b  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOMATERIALS; CHEMICAL MODIFICATION; CYCLIC VOLTAMMETRY; ENZYMES; OPTICAL SWITCHES; PROTEINS; TRANSDUCERS;

AMPEROMETRIC TRANSDUCTION; APO GLUCOSE OXIDASES; FAD COFACTORS; FLAVIN ADENINE DINUCLEOTIDES (FAD); NITROSPIROPYRAN; PHOTOSWITCHABLE BIOCATALYSTS;

CATALYSTS;

FLAVINE ADENINE NUCLEOTIDE; GLUCOSE OXIDASE;

AMPEROMETRY; ARTICLE; CATALYST; ENERGY TRANSFER; IRRADIATION; ISOMER; OXIDATION REDUCTION POTENTIAL; OXIDATION REDUCTION REACTION; PHOTOSTIMULATION; PROTEIN STRUCTURE; STEREOCHEMISTRY;

EID: 0030570259     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja960228f     Document Type: Article

References (19)

1. (a) Willner, I.; Willner, B. In Bioorganic Photochemistry; Morrison, H., Ed.; Wiley: New York, 1993; Vol. 2, pp 1-110.
2. (b) Willner, I.; Rubin, S. Angew. Chem., Int. Ed. Engl. 1996, 35, 367.
3. Willner, I.; Rubin, S.; Riklin, A. J. Am. Chem. Soc. 1991, 113, 3321.
4. Willner, I.; Lion-Dagan, M.; Marx-Tibbon, S.; Katz, E. J. Am. Chem. Soc. 1995, 117, 6581.
5. Lion-Dagan, M.; Katz, E.; Willner, I. J. Am. Chem. Soc. 1994, 116, 7913.
6. Lion-Dagan, M.; Katz, E.; Willner, I. J. Chem. Soc., Chem. Commun. 1994, 2741.
7. Lion Dagan, M.; Marx-Tibbon, S.; Katz, E.; Willner, I. Angew. Chem., Int. Ed. Engl. 1995, 34, 1604.
8. Willner, I.; Lion-Dagan, M.; Rubin, S.; Wonner, J.; Effenberger, F.; Bäuerle, P. Photochem. Photobiol. 1994, 59, 491.
9. 2O (7:3) was used as eluent.
10. Bückmann, A. F.; Erdmann, H.; Pietzsch, M.; Hall, J. M.; Banniser, J. V. In Flavins and Flavoproteins; Kuneoyagi, K., Ed.; Walter de Gruyter: Berlin, 1994; p 597.
11. Namba, K.; Suzuki, S. Bull. Chem. Soc. Jpn. 1975, 48, 1323.
12. Morris, D. L.; Buckler, R. T. In Methods in Enzymology; Langone, J. J., Van Vunakis, H., Eds.; Academic Press, Inc.: 1983: Vol. 92, Part E, pp 413-417.
13. Reconstitution was accomplished by vigorous shaking, for 24 h, of apo-glucose oxidase and 1a (molar ratio 1:10) in a phosphate buffer, 0.1 M, pH = 7.0, The product was dialyzed against phosphate buffer, 0.1 M, pH = 7.0 for 30 h.
14. 2 penetration path.
15. Bartlett, P. N.; Tebbutt, P.; Whitaker, R. G. Prog. React. Kinet. 1991, 16, 55.
16. Bourdillon, C.; Demaille, C.; Moiroux, J.; Savéant, J. M. J. Am. Chem. Soc. 1993, 115, 2.
17. Marx-Tibbon, S.; Katz, E.; Willner, I. J. Am. Chem. Soc. 1995, 117, 9925.
18. (a) Katz, E.; Schlereth, D.; Schmidt, H.-L. J. Electroanal. Chem. 1994, 367, 59.
19. (b) Riklin, A.; Willner, I. Anal. Chem. 1995, 67, 4118.