Nucleophile specificity of subtilisin in an organic solvent with low water content: Investigation via acyl transfer reactions

Biotechnology and Bioengineering

Volumn 49, Issue 5, 1996, Pages 553-558

  Čeřovský, Václav ^a   Jakubke, Hans Dieter ^b  

^a INSTITUTE OF ORGANIC CHEMISTRY AND BIOCHEMISTRY   (Czech Republic)

^b UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

acyl transfer reaction; enzymatic peptide synthesis; organic solvent; subtilisin

Indexed keywords

ACETONITRILE; AMINO ACIDS; ENZYME KINETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ORGANIC SOLVENTS; SYNTHESIS (CHEMICAL);

ACYL TRANSFER REACTIONS; ENZYMATIC PEPTIDE SYNTHESIS; LOW WATER CONTENT; NAGARSE; NUCLEOPHILE SPECIFICITY; PEPTIDES; SUBTILISIN; SUBTILOPEPTIDASE A;

ENZYMES;

ACETONITRILE; ORGANIC SOLVENT; PIPERIDINE; SUBTILISIN;

ALLOTYPE; ARTICLE; CATALYSIS; PEPTIDE SYNTHESIS; REACTION ANALYSIS;

EID: 0030569936     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19960305)49:5<553::AID-BIT8>3.0.CO;2-I     Document Type: Article

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