Coculture of genetically transformed roots and shoots for synthesis, translocation, and biotransformation of secondary metabolites

Biotechnology and Bioengineering

Volumn 49, Issue 5, 1996, Pages 481-494

  Subroto, M Ahkam ^a   Kwok, Kian H ^a   Hamill, John D ^b   Doran, Pauline M ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

^b MONASH UNIVERSITY   (Australia)

Author keywords

Atropa belladonna; biotransformation; Duboisia leichhardtii x D. myoporoides, root shoot coculture; shooty teratoma; tropane alkaloids

Indexed keywords

AMINES; BIOREACTORS; BIOSYNTHESIS; COPPER COMPOUNDS; GENETIC ENGINEERING; METABOLITES; PH; PLANTS (BOTANY);

BIOTRANSFORMATION; COCULTURE; COPPER SULPHATE; HYOSCYAMINE; ROOTS; SCOPOLAMINE; SHAKE FLASKS; SHOOTS; SHOOTY TERATOMAS; TROPANE ALKALOIDS;

TISSUE CULTURE;

BELLADONNA ALKALOID; POLOXAMER; SCOPOLAMINE;

ARTICLE; BIOTRANSFORMATION; GENETIC TRANSFORMATION; NONHUMAN; PH; PLANT GROWTH; PLANT ROOT; TISSUE CULTURE;

ATROPA BELLADONNA; DUBOISIA LEICHHARDTII; DUBOISIA MYOPOROIDES;

EID: 0030569933     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19960305)49:5<481::AID-BIT1>3.0.CO;2-F     Document Type: Article

References (41)

1. Bentley, P. K., Gates, R. M. C., Lowe, K. C., de Pomerai, D. I., Walker, J. A. L. 1989. In vitro cellular responses to a non-ionic surfactant, Pluronic F-68. Biotechnol. Lett. 11: 111-114.
2. Bhadra, R., Vani, S., Shanks, J. V. 1993. Production of indole alkaloids by selected hairy root lines of Catharanthus roseus. Biotechnol. Bioeng. 41: 581-592.
3. Deno, H., Yamagata, H., Emoto, T., Yoshioka, T., Yamada, Y., Fujita, Y. 1987. Scopolamine production by root cultures of Duboisia myoporoides. II. Establishment of a hairy root culture by infection with Agrobacterium rhizogenes. J. Plant Physiol. 131: 315-323.
4. de Vries-Uijtewaal, E., Gilissen, L. J. W., Flipse, E., Sree Ramulu, K., de Groot, B. 1988. Characterization of root clones obtained after transformation of monohaploid and diploid potato genotypes with hairy root inducing strains of Agrobacterium. Plant Sci. 58: 193-202.
5. Eapen, S., Rangan, T. S., Chadha, M. S., Heble, M. R. 1978. Morphogenetic and biosynthetic studies on tissue cultures of Atropa belladonna L. Plant Sci. Lett. 13: 83-89.
6. Ermayanti, T. M., McComb, J. A., O'Brien, P. A. 1994. Stimulation of synthesis and release of swainsonine from transformed roots of Swainsona galegifolia. Phytochemistry 36: 313-317.
7. Furze, J. M., Rhodes, M. J. C., Parr, A. J., Robins, R. J., Withehead, I. M., Threlfall, D. R. 1991. Abiotic factors elicit sesquiterpenoid phytoalexin production but not alkaloid production in transformed root cultures of Datura stramonium. Plant Cell Rep. 10: 111-114.
8. Gritsanapan, W., Griffin, W. J. 1992. Alkaloids and metabolism in a Duboisia hybrid. Phytochemistry 31: 471-477.
9. Gritsanapan, W., Griffin, W. J. 1992. Alkaloid metabolism in callus and regenerating shoot cultures of a Duboisia hybrid. Phytochemistry 31: 3069-3071.
10. Hamilton, R. M., Lang, J., Pedersen, H., Chin, C.-K. 1986. Secondary metabolites from organized Atropa belladonna cultures. Biotechnol. Bioeng. Symp. 17: 685-698.
11. Handa-Corrigan, A., Emery, A. N., Spier, R. E. 1989. Effect of gas-liquid interfaces on the growth of suspended mammalian cells: Mechanism of cell damage by bubbles. Enzyme Microb. Technol. 11: 230-235.
12. Hartmann, T., Witte, L., Oprach, F., Toppel, G. 1986. Reinvestigation of the alkaloid composition of Atropa belladonna plants, root cultures, and cell suspension cultures. Planta Med. 52: 390-395.
13. Hashimoto, T., Hayashi, A., Amano, Y., Kohno, J., Iwanari, H., Usuda, S., Yamada, Y. 1991. Hyoscyamine 6β-hydroxylase, an enzyme involved in tropane alkaloid biosynthesis, is localized at the pericycle of the root. J. Biol. Chem. 266: 4648-4653.
14. Hashimoto, T., Yun, D.-J., Yamada, Y. 1993. Production of tropane alkaloids in genetically engineered root cultures. Phytochemistry 32: 713-718.
15. Hashimoto, T., Yamada. Y. 1994. Alkaloid biogenesis: Molecular aspects. Annu. Rev. Plant Physiol. Plant Mol. Biol. 45: 257-285.
16. Jardin, B., Tom, R., Chavarie, C., Rho, D., Archambault, J. 1991. Stimulated indole alkaloid release from Catharanthus roseus immobilized cultures: Initial studies. J. Biotechnol. 21: 43-62.
17. Jung, G., Tepfer, D. 1987. Use of genetic transformation by the Ri T-DNA of Agrobacterium rhizogenes to stimulate biomass and tropane alkaloid production in Atropa belladonna and Calystegia sepium roots grown in vitro. Plant Sci. 50: 145-151.
18. Kamada, H., Okamura, N., Satake, M., Harada, H., Shimomura, K. 1986. Alkaloid production by hairy root cultures in Atropa belladonna. Plant Cell Rep. 5: 239-242.
19. Khatun, A., Laouar, L., Davey, M. R., Power, J. B., Mulligan, B. J., Lowe, K. C. 1993. Effects of Pluronic F-68 on shoot regeneration from cultured jute cotyledons and on growth of transformed roots. Plant Cell Tiss. Org. Cult. 34: 133-140.
20. Kitamura, Y. 1988. Dubolsia spp.: In vitro regeneration, and the production of tropane and pyridine alkaloids. pp. 419-436. In: Y. P. S. Bajaj (ed.), Biotechnology in agriculture and forestry, Vol. 4: Medicinal and aromatic plants I. Springer-Verlag, Berlin.
21. Kumar, V., Laouar, L., Davey, M. R., Mulligan, B. J., Lowe, K. C. 1991. Effects of Pluronic F-68 on callus growth and protoplast plating efficiency of Solanum dulcamara. Plant Cell Rep. 10: 52-54.
22. Kwok, K. H., Doran, P. M. 1995. Kinetic and stoichiometric analysis of hairy roots in a segmented bubble column reactor. Biotechnol. Prog. 11: 429-435.
23. Mano, Y. 1993. Transformation in Duboisia spp. pp. 190-201. In: Y. P. S. Bajaj (ed.), Biotechnology in agriculture and forestry, Vol. 22: Plant protoplasts and genetic engineering III. Springer-Verlag, Berlin.
24. Mizrahi, A. 1975. Pluronic polyols in human lymphocyte cell line cultures. J. Clin. Microbiol. 2: 11-13.
25. Mukundan, U., Hjortso, M. A. 1991. Growth and thiophene accumulation by hairy root cultures of Tagetes patula in media of varying initial pH. Plant Cell Rep. 9: 627-630.
26. Murhammer, D. W., Goochee, C. F. 1988. Scaleup of insect cell cultures: Protective effects of Pluronic F-68. Bio/Technology 6: 1411-1418.
27. Renaudin, J.-P., Guern, J. 1982. Compartmentation mechanisms of indole alkaloids in cell suspension cultures of Catharanthus roseus. Physiol. Vég. 20: 533-547.
28. Robins, R. J., Walton, N. J., Hamill, J. D., Parr, A. J., Rhodes, M. J. C. 1991. Strategies for the genetic manipulation of alkaloid-producing pathways in plants. Planta Med. (Suppl. 1) 57: S27-S35.
29. Saito, K., Murakoshi, I., Inzé, D., van Montagu, M. 1989. Biotransformation of nicotine alkaloids by tobacco shooty teratomas induced by a Ti plasmid mutant. Plant Cell Rep. 7: 607-610.
30. Saito, K., Yamazaki, M., Kawaguchi, A., Murakoshi, I. 1991. Metabolism of solanaceous alkaloids in transgenic plant teratomas integrated with genetically engineered genes. Tetrahedron 47: 5955-5968.
31. Sevón, N., Varjonen, T., Hiltunen, R., Oksman-Caldentey, K.-M. 1992. Effect of sucrose, nitrogen, and copper on the growth and alkaloid production of transformed root cultures of Hyoscyamus muticus. Planta Med. (Suppl. 1) 58: A609-A610.
32. Sharp, J. M., Doran, P. M. 1990. Characteristics of growth and tropane alkaloid synthesis in Atropa belladonna roots transformed by Agrobacterium rhizogenes. J. Biotechnol 16: 171-186.
33. Simola, L. K., Nieminen, S., Huhtikangas, A., Ylinen, M., Naaranlahti, T., Lounasmaa, M. 1988. Tropane alkaloids from Atropa belladonna, Part II. Interaction of origin, age, and environment in alkaloid production of callus cultures. J. Nat. Prod. 51: 234-242.
34. Subroto, M. A., Hamill, J. D., Doran, P. M. 1996. Development of shooty teratomas from several solanaceous plants: Growth kinetics, stoichiometry and alkaloid production. J. Biotechnol. 45: 45-57.
35. Tsoulpha, P., Doran, P. M. 1991. Solasodine production from self-immobilised Solanum aviculare cells. J. Biotechnol. 19: 99-110.
36. Waller, G. R., Nowacki, E. K. 1978. Alkaloid biology and metabolism in plants. Plenum, New York.
37. West, F. R., Mika, E. S. 1957. Synthesis of atropine by isolated roots and root-callus cultures of belladonna. Bot. Gaz. 119: 50-54.
38. Wilson, P. D. G., Hilton, M. G., Robins, R. J., Rhodes, M. J. C. 1987. Fermentation studies of transformed root cultures. pp. 38-51. In: G. W. Moody and P. B. Baker (eds.), Bioreactors and biotransformations. Elsevier Applied Science, London.
39. Yamada, Y., Endo, T. 1984. Tropane alkaloid production in cultured cells of Duboisia leichhardtii. Plant Cell Rep. 3: 186-188.
40. Yamada, Y., Hashimoto, T. 1988. Biosynthesis of tropane alkaloids. pp. 199-212. In: G. Bock and J. Marsh (eds.), Applications of plant cell and tissue culture. Wiley, Chichester.
41. Yun, D.-J, Hashimoto, T., Yamada, Y. 1992. Metabolic engineering of medicinal plants: Transgenic Atropa belladonna with an improved alkaloid composition. Proc. Natl. Acad. Sci. USA 89: 11799-11803.