Nuclear calcium flux in Trypanosoma brucei can be quantified with targeted aequorin

Molecular and Biochemical Parasitology

Volumn 83, Issue 1, 1996, Pages 57-67

  Xiong, Zhao Hui ^a   Ruben, Larry ^a  

^a SOUTHERN METHODIST UNIVERSITY   (United States)

Author keywords

calcium flux; nucleus; targeted aequorin; Trypanosoma brucei

Indexed keywords

CALCIUM ION;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CALCIUM TRANSPORT; CELL NUCLEUS; CONTROLLED STUDY; INTRACELLULAR TRANSPORT; NONHUMAN; PRIORITY JOURNAL; TRYPANOSOMA BRUCEI;

AEQUORIN; ANIMALS; BIOLOGICAL TRANSPORT; CALCIUM; CELL COMPARTMENTATION; CELL MEMBRANE; CELL NUCLEUS; CYTOPLASM; FLUORESCENT ANTIBODY TECHNIQUE; LUMINESCENT MEASUREMENTS; RECOMBINANT PROTEINS; TRYPANOSOMA BRUCEI BRUCEI;

EID: 0030566706     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(96)02750-8     Document Type: Article

References (42)

1. [1] Ruben, L.R. Akins, C.D., Haghighat, N.G. and Xue, L. (1996) Calcium influx in Trypanosoma brucei can be induced by amphiphilic peptides and amines. Mol. Biochem. Parasitol. 81, 191-200.
2. [2] Ruben, L., Hutchinson, A. and Moehlman, J. (1991) Calcium homeostasis in Trypanosoma brucei. Identification of a pH-sensitive non-mitochondrial calcium pool. J. Biol. Chem. 266, 24351-24358.
3. [3] Ruben, L. and Akins, C.D. (1992) Trypanosoma brucei: the tumor promoter thapsigargin stimulates calcium release from an intracellular compartment in slender bloodstream forms. Exp. Parasitol. 74, 332-339.
4. 2+ release. J. Biol. Chem. 267, 6020-6026.
5. 2+ release from acidocalcisomes of Trypanosoma brucei. (1996) Biochem. J. 315, 265-270.
6. [6] Ruben, L., Egwuagu, C. and Patton, C.L. (1983) African trypanosomes contain calmodulin which is distinct from host calmodulin. Biochim. Biophys. Acta 758, 104-113.
7. [7] Wu, Y., DeFord, J., Benjamin, R., Lee, M. G.-S. and Ruben, L. (1994) The gene family of EF-hand calcium-binding proteins from the flagellum of Trypanosoma brucei. Biochem. J. 304, 833-841.
8. [8] Kaur, K.J. and Ruben, L. (1994) Protein translation elongation factor-1χ from Trypanosoma brucei binds calmodulin. J. Biol. Chem. 269, 23045-23050.
9. [9] Ridgley, E.L., Xiong, Z., Kaur, K.J. and Ruben, L. (1996) Genomic organization and expression of elongation factor-1χ genes in Trypanosoma brucei. Molec. Biochem. Parasitol. 79, 119-123.
10. [10] Paindavoine, P., Rolin, S., Van Assel, S., Geuskens, M., Jauniaux, J-C., Dinsart, C., Huet, G. and Pays, E. (1992) A gene from the variant surface glycoprotein expression site encodes one of several transmembrane adenylate cyclases located on the flagellum of Trypanosoma brucei. Mol. Cell. Biol. 12, 1218-1225.
11. [11] Wong, S., Kretsinger, R.H. and Campbell, D.A. (1992) Identification of a new EF-hand superfamily member from Trypanosoma brucei. Mol. Gen. Genetics. 233, 225-230.
12. 2+ concentration measured with specifically targeted recombinant aequorin. EMBO J. 12, 4813-4819.
13. [13] Badminton, M.N., Kendall, J.M., Sala-Newby, G. and Campbell, A.K. (1995) Nucleoplasmin-targeted aequorin provides evidence for a nuclear calcium barrier. Exp. Cell Res. 216, 236-243.
14. [14] Minamikawa, T., Takahashi, A. and Fujita, S. (1995) Differences in features of calcium transients between the nucleus and the cytosol in cultured heart muscle cells: analyzed by confocal microscopy. Cell Calcium 17, 165-176.
15. [15] Gilchrist, J.S.C., Czubryt, M.P. and Pierce, G.N. (1994) Calcium and calcium-binding proteins in the nucleus. Mol. Cell. Biochem. 135, 79-88.
16. [16] Bachs, O., Agell, N. and Carafoli, E. (1994) Calmodulin and calmodulin-binding proteins in the nucleus. Cell Calcium 16, 289-296.
17. [17] Johnson, F.H. and Shimomura, O. (1978) Introduction to the bioluminescence of medusae, with special reference to the photoprotein aequorin. Methods Enzymol. 57, 271-291.
18. [18] Inouye, S., Noguchi, M., Sakaki, Y., Takagi, Y., Miyata, T., Iwanaga, S., Miyata, T. and Tsuji, F.I. (1985) Cloning and sequence analysis of cDNA for the luminescent protein aequorin. Proc. Natl. Acad. Sci. USA 82, 3154-3158.
19. [19] Shimomura, O. (1995) Luminescence of aequorin is triggered by the binding of two calcium ions. Biochem. Biophys. Res. Commun. 211, 359-363.
20. [20] Blinks, J.R., Mattingly, P.H., Jewell, B.R., van Leeuwen, M., Harrer, G.C. and Allen, D.G. (1979) Practical aspects of the use of aequorin as a calcium indicator: assay, preparation, microinjection, and interpretation of signals. Methods Enzymol. 57, 292-328.
21. 2+ ion indicators. Biochem. J. 270, 309-312.
22. 2+ in Escherichia coli. FEBS Lett. 282, 405-408.
23. c). A critical evaluation. J. Biol. Chem. 270, 9896-9903.
24. [24] Kendall, J.M., Dormer, R.L. and Campbell, A.K. (1990) Targeting aequorin to the endoplasmic reticulum of living cells. Biochem. Biophys. Res. Commun. 189, 1008-1016.
25. 2+ revealed by specifically targeted recombinant aequorin. Nature 358, 325-327.
26. 3-sensitive channels that are sensed by neighboring mitochondria. Science 262, 744-747.
27. 2+ homeostasis in intact cells. J. Cell Biol. 126, 1183-1194.
28. 2+ to supramicromolar levels in a pancreatic β-cell line. Possible role in glucose and agonist-induced insulin secretion. J. Biol. Chem. 268, 22385-22390.
29. [29] Metzenberg, S., Joblet, C., Verspieren, P., and Agabian, N. (1993) Ribosomal protein L25 from Trypanosoma brucei: phylogeny and molecular co-evolution of an rRNA-binding protein and its rRNA binding site. Nuc. Acids. Res. 21, 4936-4940.
30. [30] Brun, R. and Schonenberger, M. (1979) Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Acta Trop. 36, 289-292.
31. [31] Priest, J.W. and Hajduk, S. (1996) In vitro import of the Rieske iron sulfur protein by trypanosome mitochondria. J. Biol. Chem. 271, 20060-20069.
32. [32] Olmstead, J.B. (1981) Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples. J. Biol. Chem. 256, 11955-11957.
33. [33] Sommer, J.M., Cheng, C.L., Keller, G.A. and Wang, C.C. (1992) In vivo import of firefly luciferase into the glycosomes of Trypanosoma brucei and mutational analysis of the C-terminal targeting signal. Mol. Biol. Cell 3, 749-759.
34. [34] Mousli, M. Bronner, C., Landry, Y., Bockaert, J. and Rouot, B. (1990) Direct activation of GTP-binding regulatory proteins (G-proteins) by substance P and compound 48/80. FEBS Lett. 259, 260-262.
35. 2 from human platelets. Biochim. Biophys. Acta. 920, 301-305.
36. [36] Gietzen, K., Adamizyk-Englemann, P., Wuthrich, A., Konstantinova, A. and Bader, H. (1983) Compound 48/ 80 is a selective and powerful inhibitor of calmodulin-regulated functions. Biochim. Biophys. Acta. 736, 109-118.
37. [37] Allen, D.G., Blinks, J.R. and Prendergast, F.G. (1977) Aequorin luminescence: relation of light emission to calcium concentration-a calcium-independent component. Science 195, 996-998.
38. 2+] changes in nucleus and cytosol. Cell Calcium 16, 239-246.
39. 2+ concentration in intact cells. Cell Calcium 16, 259-268.
40. [40] Hepler, P.K. (1994) The role of calcium in cell division. Cell Calcium 16, 322-330.
41. [41] Roche, E. and Prentki, M. (1994) Calcium regulation of immediate-early response genes. Cell Calcium 16, 331-338.
42. [42] Nicotera, P., Zhivotovsky, B. and Orrenius, S. (1994) Nuclear calcium transport and the role of calcium in apoptosis. Cell Calcium 16, 279-288.