Production and purification of Clostridium perfringens alpha-toxin using a protein-hyperproducing strain, Bacillus brevis 47

FEMS Microbiology Letters

Volumn 145, Issue 2, 1996, Pages 239-243

  Nagahama, Masahiro ^a   Michiue, Kei ^a   Sakurai, Jun ^a  

^a TOKUSHIMA BUNRI UNIVERSITY   (Japan)

Author keywords

Alpha toxin; Bacillus brevis; Bacillus subtilis; Clostridium perfringens; Gene

Indexed keywords

CLOSTRIDIUM TOXIN;

AMINO ACID SEQUENCE; ARTICLE; BREVIBACILLUS BREVIS; CLOSTRIDIUM PERFRINGENS; CONTROLLED STUDY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; TOXIN SYNTHESIS;

AMINO ACID SEQUENCE; BACILLUS; BACTERIOLOGICAL TECHNIQUES; BASE SEQUENCE; CLOSTRIDIUM PERFRINGENS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; PHOSPHOLIPASE C; TRANSFORMATION, GENETIC;

BACILLUS SUBTILIS; BREVIBACILLUS BREVIS; CLOSTRIDIUM; CLOSTRIDIUM PERFRINGENS;

EID: 0030561508     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(96)00416-8     Document Type: Article

References (12)

1. [1] McDonel, J.L. (1986) Toxins of Clostridium perfringens types A, B, C, D and E. In: Pharmacology of Bacterial Toxins (Dorner, F. and Drews, J., Eds.), pp. 477-517, Pergamon Press, Oxford.
2. [2] Sakurai, J. (1995) Toxins of Clostridium perfringens. Rev. Med. Microbiol. 6, 175-185.
3. [3] Titball, R.W. (1993) Bacterial phospholipases C. Microbiol. Rev. 57, 347-366.
4. [4] Nagahama, M., Okagawa, Y., Nakayama, T., Nishioka, E. and Sakurai, J. (1995) Site-directed mutagenesis of histidine residues in Clostridium perfringens alpha-toxin. J. Bacteriol. 177, 1179-1185.
5. [5] Hough, E., Hansen, L.K., Birknes, B., Jynge, K., Hansen, S., Hordvik, A., Little, C., Dodson, E. and Derewenda, Z. (1989) High-resolution (1.5 Å) crystal structure of phospholipase C from Bacillus cereus. Nature 338, 357-360.
6. [6] Nagahama, M., Iida, H., Nishioka, E., Okamoto, K. and Sakurai, J. (1994) Roles of the carboxy-terminal region of Clostridium perfringens alpha toxin. FEMS Microbiol. Lett. 120, 297-302.
7. [7] Udaka, S. and Yamagata, H. (1993) High-level secretion of heterologous proteins by Bacillus brevis. Methods Enzymol. 217, 23-33.
8. [8] Yamagata, H., Nakahama, K., Suzuki, Y., Kakinuma, A., Tsukagoshi, N. and Udaka, S. (1989) Use of Bacillus brevis for efficient synthesis and secretion of human epidermal growth factor. Proc. Natl. Acad. Sci. USA 86, 3589-3593.
9. [9] Tamura, H., Tameishi, K., Yamagata, H., Udaka, S., Kobayashi, T., Tomita, M. and Ikezawa, H. (1992) Mass production of sphingomyelinase of Bacillus cereus by a protein-hyperproducing strain, Bacillus brevis 47, and its purification. J. Biochem. 112, 488-491.
10. [10] Ichikawa, Y., Yamagata, H., Tochikubo, K. and Udaka, S. (1993) Very efficient extracellular production of cholera toxin B subunit using Bacillus brevis. FEMS Microbiol. Lett. 111, 219-224.
11. [11] Hirata, Y., Minami, J., Koyama, M., Matsushita, O., Katayama, S., Jin, F., Maeta, H. and Okabe, A. (1995) A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells. Appl. Environ. Mrcrobiol. 61, 4114-4115.
12. [12] Nagahama, M., Michiue, K. and Sakurai, J. (1996) Membrane-damaging action of Clostridium perfringens alpha-toxin on phospholipid liposomes. Biochim. Biophys. Acta 1280, 120-126.