Modification of the plasmid initiator protein RepC active site during replication

FEMS Microbiology Letters

Volumn 145, Issue 2, 1996, Pages 245-253

  Rasooly, Avraham ^a,c   Rasooly, Rebekah S ^b  

^a NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b ST JOHN'S UNIVERSITY   (United States)

^c CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

Histidine tagging; Plasmid; Post translational modification; PT181; Replication protein; Staphylococcus aureus

Indexed keywords

HISTIDINE; OLIGONUCLEOTIDE;

ARTICLE; CONTROLLED STUDY; DNA REPLICATION; MOLECULAR MODEL; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN MODIFICATION; PROTEIN SYNTHESIS; STAPHYLOCOCCUS AUREUS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BINDING SITES; CLONING, MOLECULAR; DNA REPLICATION; GENES, BACTERIAL; HISTIDINE; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDES; PLASMIDS; PROMOTER REGIONS (GENETICS); PROTEIN PROCESSING, POST-TRANSLATIONAL; STAPHYLOCOCCUS AUREUS;

STAPHYLOCOCCUS AUREUS;

EID: 0030561293     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(96)00419-3     Document Type: Article

References (17)

1. [1] Scott, J.R. (1984) Regulation of plasmid replication. Microbiol. Rev. 48, 1-23.
2. [2] Manch-Citron, J., Gennaro, M.L., Majumder, S. and Novick, R.P. (1986) RepC is rate-limiting for pT181 plasmid replication. Plasmid 16, 108-115.
3. [3] Khan, S.A., Adler, G.K. and Novick, R.P. (1982) Functional origin of replication of pT181 plasmid DNA is contained within a 168-base-pair segment. Proc. Natl. Acad. Sci. USA 79, 4580-4594.
4. [4] Khan, S.A. and Novick, R.P. (1983) Complete nucleotide sequence of pT181, a tetracycline resistance plasmid from Staphylococcus aureus. Plasmid 10, 151-159.
5. [5] Kumar, C.C. and Novick, R.P. (1985) Plasmid pT181 replication is regulated by two countertranscripts. Proc. Natl. Acad. Sci. USA 82, 638-642.
6. [6] Koepsel, R.R. and Khan, S.A. (1987) Cleavage of single-stranded DNA by plasmid pT181-encoded RepC protein. Nucleic Acids Res. 15, 4085-4097.
7. [7] Thomas, C.D., Balson, D.F. and Shaw, W.V. (1990) In vitro studies of the initiation of staphylococcal plasmid replication. J. Biol. Chem. 265, 5519-5530.
8. [8] Rasooly, A. and Novick, R.P. (1993) Replication-specific inactivation of the pT181 plasmid initiator protein. Science 262, 1048-1050.
9. [9] Rasooly, A., Wang, P.Z. and Novick, R.P. (1994) Replication-specific conversion of the Staphylococcus aureus pT181 initiator protein from an active homodimer to an inactive heterodimer. EMBO J. 13, 5245-5251.
10. [10] Rasooly, A., Projan, S.J. and Novick, R.P. (1994) Plasmids of the pT181 family show replication-specific initiator protein modification. J. Bacteriol. 176, 2450-2453.
11. [11] Muller, A., Rojo, F. and Alonso, J. (1995) The level of the pUB110 replication initiator protein is autoregulated, which provides an additional control for plasmid copy number. Nucleic Acids Res. 23, 1894-1900.
12. [12] Koepsel, R.R., Murray, R.W., Rosenblum, W.D. and Khan, S.K. (1985) Purification of pT181-encoded repC protein required for the initiation of plasmid replication. J. Biol. Chem. 260, 8571-8577.
13. [13] Iordanescu, S. (1989) Specificity of the interactions between the Rep proteins and the origins of replication of Staphylococcus aureus plasmids pT181 and pC221. Mol. Gen. Genet. 217, 481-487.
14. [14] Chang, S. and Cohen, S.C. (1979) High frequency of transformation of Bacillus subtilis protoplast by plasmid DNA. Mol. Gen. Genet. 168, 111-115.
15. [15] Khan, S.A., Carleton, S.M. and Novick, R.P. (1981) Replication of plasmid pT181 DNA in vitro, requirement for a plasmid-encoded product. Proc. Natl. Acad. Sci. USA 78, 4902-4906.
16. [16] Gharahdaghi, F., Kirchner, M., Fernandez, J. and Mische, S.M. (1996) Peptide-mass profiles of polyvinylidene difluoride-bound proteins by matrix-assisted laser desorption ionization time-of-flight mass spectrometry in the presence of non-ionic detergents. Anal. Biochem. 233, 94-99.
17. [17] Novick, R.P. (1991) Genetic systems in staphylococci. Methods Enzymol. 204, 587-636.