A novel technique for quantitative detection of mRNA expression in human bone derived cells cultured on biomaterials

Journal of Biomedical Materials Research

Volumn 33, Issue 4, 1996, Pages 217-223

  Zreiqat, Hala ^a   Markovic, Boban ^a   Walsh, William R ^a   Howlett, C Rolfe ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ALUMINA; ANTIGENS; BONE; CELLS; CERAMIC MATERIALS; COLLAGEN; ENZYMES; IN SITU PROCESSING; MONOCLONAL ANTIBODIES; PLASTICS; RNA; TISSUE CULTURE;

ALKALINE PHOSPHATASE; IN SITU HYBRIDIZATION TECHNIQUE; OSTEOCALCIN; OSTEOPONTIN; PROTEIN EXPRESSION; RNA EXPRESSION;

BIOMATERIALS;

ALKALINE PHOSPHATASE; BIOMATERIAL; COLLAGEN TYPE 1; COLLAGEN TYPE 2; MESSENGER RNA; OSTEOCALCIN; OSTEOPONTIN;

ANALYTIC METHOD; ARTICLE; BONE CELL; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; PROTEIN EXPRESSION; QUANTITATIVE DIAGNOSIS; REPRODUCIBILITY; RNA ANALYSIS;

ALKALINE PHOSPHATASE; ALUMINUM OXIDE; ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, MYELOMONOCYTIC; BIOCOMPATIBLE MATERIALS; BONE AND BONES; CELLS, CULTURED; COLLAGEN; CULTURE TECHNIQUES; DNA PROBES; HUMANS; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; MACROPHAGES; OSTEOCALCIN; OSTEOPONTIN; RNA, MESSENGER; SIALOGLYCOPROTEINS; TRANSCRIPTION, GENETIC;

EID: 0030560806     PISSN: 00219304     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4636(199624)33:4<217::AID-JBM2>3.0.CO;2-S     Document Type: Article

References (25)

1. Schwartz, Z.; Boyan, B. Underlying mechanisms at the bone-biomaterial interface. J. Cell. Biomed. Mater. Res. 26:340-347; 1994.
2. Steele, J. G.; Johnson, G.; Underwood, A. Role of serum vitronectin and fibronectin in adhesion of fibroblasts following seeding onto tissue culture polystyrene. J. Biomed. Mater. Res. 26:861-884; 1992.
3. Malik, M. A.; Puleo, D. A.; Bizios, R.; Doremus. R. H. Osteoblasts on hydroxyapatite, alumina and bone surfaces in vitro: morphology during the first 2 h of attachment. Biomaterials 13:123-128; 1992.
4. Neo, M.; Kotani, S.; Figita, Y.; Nakamura, T.; Yamamura, T. Differences in ceramic-bone interface between surface active ceramics and resorbable ceramics: a study by scanning and transmission electron microscopy. J. Biomed. Mater. Res. 26:255-267; 1992.
5. Puleo, D. A.; Preston, K.; Shaffer, J.; Bizios, R. Examination of osteoblast-orthopaedic biomaterial interactions using molecular techniques. Biomaterials 14:111-114; 1993.
6. Zou, L.; Walsh, W. R.; Keeping, H.; et al. Osteoblast-orthopaedic biomaterial response. Mikos, A. G.; Murphy, R. M.; Bernstein, H.; Peppas, N. A. eds. Biomaterials for Drug and Cell Delivery, Vol. 331. Pittsburgh, PA: Materials Research Society; 1994:127-131.
7. Bellows, C.; Aubin, J.; Heerche, J.; Antoz, M. Mineralized bone nodules formed in vitro from enzymatically released rat calvaria cell populations. Calcif. Tissue. Int. 38:143-154; 1986.
8. Gehron Robey, P.; Termine, J. D. Human bone cells in vitro. Calcif. Tissue Int. 37:453-460; 1985.
9. Brown, I. G.; Gavin, J. E.; Gavin, B. F.; MacGill, R. A. Metal vapour vacuum arch ion source. Rev. Sci. Instrum. 57:1069-1084; 1986.
10. Markovic, B.; Wu, Z. H.; Chesterman, C. N.; Chong, B. H. Quantitation of FcγRII mRNA in platelets and megakaryoblastic cell lines by a new method of in situ hybridization. J. Immunol. Methods 172:105-114; 1994.
11. 1 consortium linkage map of human chromosome 1. Genomics 9:686-700; 1991.
12. Villarreal, X. C.; Mann, K. G.; Long, G. L. Structure of human osteonectin based upon analysis of cDNA and genomic sequences. Biochemistry 28:6483-6491; 1989.
13. Kiefer, M. C.; Bauer, D. M.; Barr, P. J. The cDNA and derived amino acid sequence for human osteopontin. Nucleic Acids Res. 17:3306; 1989.
14. Myers, J. C.; Chu, M.-L.; Faro, S. H.; Clark, W. J.; Prokop, D. J.; Ramirez, F. Cloning a cDNA for the pro-α2 chain of human type I collagen. Proc. Natl. Acad. Sci. (USA) 78: 3516-3520; 1981.
15. Erba, H. P.; Gunning, P.; Kedes, L. Nucleotide sequence of the human gamma cytoskeletal actin mRNA: anomalous evolution of vertebrate non-muscle actin genes. Nucleic Acids Res. 14:5275-5294; 1986.
16. Forster, A. C.; Mclnnes, J. L.; Skingle, D. C.; Symons, R. H. Non radioactive hybridization of probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin. Nucleic Acid Res. 13:745-761; 1985.
17. Pringle, J. H.; Homer, C. E.; Warford, A.; Kendall, C. H.; Lauder, I. In situ hybridization: alkaline phosphatase visualisation of biotinylated probes in cryostat and paraffin sections. Histochem. J. 19:488-496; 1987.
18. Wilcheck, M.; Bayer, E. A. The avidin-biotin complex in bioanalytical applications. Anal. Biochem. 171:1-32; 1988.
19. Termine, J. D.; Belcourt, A. B.; Conn, K. M.; Kleinmann, H. K. Mineral and collagen-binding proteins of fetal calf bone. J. Biol. Chem. 256:10403-10408; 1981.
20. Rodan, G. A.; Rodan, S. B. Expression of the osteoblastic phenotype. Peck, W. A. eds. Bone and Mineral Research, Vol. 2. Amsterdam: Elsevier Science; 1984:244-285.
21. Howlett, C. R.; Zreiqat, H.; O'Dell, R.; et al. The effect of magnesium ion implantation into alumina upon the adhesion of human bone derived cells. J. Mater. Sci. Mater. Med. 5:715-722; 1994.
22. Vrouwenvelder, W. C. A.; Groot, C. G.;deGroot, K. Behaviour of fetal rat osteoblasts cultured in vitro on bioactive glass and nonreactive glasses. Biomaterials 13:382-392; 1992.
23. Owen, T.; Aronow, M.; Shalhoub, V.; et al. Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J. Cell. Physiol. 143:420-430; 1990.
24. Standard, O. C.; Zreiqat, H.; Kelly, J.; Howlett, C. R. The effect of topography of alumina on the in vitro attachment of bone cells. J. Austrolasia Ceram. Soc. 29:103-109; 1993.
25. Pulford, K. A. F.; Sipos, A.; Cordell, J. L.; Stross, W. P.; Mason, D. Y. The distribution of the CD68 macrophage/myeloid associated antigen. Int. Immunol. 2:973-980; 1990.