SV40 large T immortalised cell lines of the rat blood-brain and blood-retinal barriers retain their phenotypic and immunological characteristics

Journal of Neuroimmunology

Volumn 71, Issue 1-2, 1996, Pages 51-63

  Greenwood, J ^a   Pryce, G ^a   Devine, L ^a   Male, D K ^b   Dos Santos, W L C ^b   Calder, V L ^a   Adamson, P ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

blood brain barrier; blood retinal barrier; endothelium; immortalisation; retinal pigment epithelium

Indexed keywords

CD31 ANTIGEN; CYTOKERATIN; GLYCOPROTEIN P; INTERCELLULAR ADHESION MOLECULE 1; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2; VASCULAR CELL ADHESION MOLECULE 1; VIRUS LARGE T ANTIGEN;

ANIMAL CELL; ARTICLE; BLOOD BRAIN BARRIER; BLOOD RETINA BARRIER; CELL LINE; ENDOTHELIUM; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; RAT; SIMIAN VIRUS 40; T LYMPHOCYTE;

ANIMALS; ANTIGENS, POLYOMAVIRUS TRANSFORMING; ANTIGENS, SURFACE; BIOLOGICAL TRANSPORT; BLOOD-BRAIN BARRIER; CELL ADHESION MOLECULES; CELL MOVEMENT; CELL TRANSFORMATION, VIRAL; ENDOTHELIUM; ENDOTHELIUM, VASCULAR; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HISTOCOMPATIBILITY ANTIGENS; LIPOPROTEINS, LDL; PIGMENT EPITHELIUM OF EYE; RATS; RETINA; SIMIAN VIRUS 40; T-LYMPHOCYTES;

EID: 0030560709     PISSN: 01655728     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-5728(96)00130-0     Document Type: Article

References (39)

1. Abbott N.J., Hughes C.C.W., Revest P.A., Greenwood J. Development and characterisation of a rat brain capillary endothelial culture: Towards an in vitro blood-brain barrier. J. Cell. Sci. 103:1992;23-37.
2. Abbott, N.J., Couraud, P-O., Roux, F. and Begley, D.J. (1995) Studies on an immortalised brain endothelial cell line: Characterization, permeability and transport. In: J. Greenwood, D. Begley and M. Segal (Eds.), New Concepts of a Blood-Brain Barrier. Plenum, New York, pp. 239-249.
3. Adamson P., Couraud P.-O., Durieu-Trautmann O., Greenwood J. ICAM-1 mediated signalling in CNS endothelial cells. Immunology. 86(Suppl. 1):1995;44.
4. Bourdoulous S., Béraud E., Le Page C., Zamora A.J., Ferry A., Bernard D., Strosberg A.D., Couraud P.-O. Anergy induction in encephalitogenic T cells is inhibited by interleukin-1. Eur. J. Immunol. 25:1995;1176-1183.
5. Butt A.M., Jones H.C. Effect of histamine and antagonists on electrical resistance across the blood-brain barrier in rat brain-surface microvessels. Brain Res. 569:1992;100-105.
6. Calder, V. and Greenwood, J. (1995). Role of the vascular endothelium in immunologically-mediated neurological disease. In: C. Savage and J. Pearson (Eds.), Immunological Aspects of the Vascular Endothelium. Cambridge University Press, Cambridge, pp. 96-121.
7. Chang C.W., Roque R.S., Defoe D.M., Caldwell R.B. An improved method for isolation and culture of pigment epithelial cells from rat retina. Curr. Eye Res. 10:1991;1081-1086.
8. Crone C., Olesen S.-P. Electrical resistance of brain microvascular endothelium. Brain Res. 241:1982;49-55.
9. Devine L., Lightman S., Greenwood J. Lymphocyte migration across the anterior and posterior blood-retinal barrier in vitro. Cell. Immunol. 168:1996;267-275.
10. Devine L., Lightman S.L., Greenwood J. Role of LFA-1, ICAM-1, VLA-4 and VCAM-1 in lymphocyte migration across retinal pigment epithelial monolayers in vitro. Immunology. 88:1996;456-462.
11. Duijvestijn A.M., Van Goor H., Klatter F., Majoor G.D., Van Bussel E., Van Breda-Vriesman P.J.C. Antibodies defining rat endothelial cells: RECA-1, a pan-endothelial cell-specific monoclonal antibody. Lab. Invest. 66:1992;459-466.
12. Durieu-Trautmann O., Chaverot N., Cazaubon S., Strosberg A.D., Couraud P.O. Intercellular adhesion molecule-1 activation induces tyrosine phosphorylation of the cytoskeleton-associated protein cortactin in brain microvessel endothelial cells. J. Biol. Chem. 269:1994;12536-12540.
13. Fickling S.A., Tooze J.A., Whitley G.S.J. Characterisation of human umbilical vein endothelial cell lines produced by transfection with the early region of SV40. Exp. Cell. Res. 201:1992;517-521.
14. Flaris N.A., Densmore T.L., Molleston M.C., Hickey W.F. Characterization of microglia and macrophages in the central nervous system of rats: Definition of the differential expression of molecules using standard and novel monoclonal antibodies in normal CNS and four models of parenchymal reaction. Glia. 7:1993;34-40.
15. Greenwood J. Astrocytes, cerebral endothelium and cell culture: The pursuit of an in vitro blood-brain barrier. Ann. NY Acad. Sci. USA. 633:1991;426-431.
16. Greenwood, J. (1992a) Experimental manipulation of the blood-brain and blood-retinal barriers. In: M.W.B. Bradbury (Ed.), Handbook of Experimental Pharmacology. Physiology and Pharmacology of the Blood-Brain Barrier, Vol. 103. Springer-Verlag, Berlin, pp. 459-486.
17. Greenwood J. Characterization of a rat retinal endothelial cell culture and the expression of P-glycoprotein in brain and retinal endothelium in vitro. J. Neuroimmunol. 39:1992;123-132.
18. Greenwood J., Calder V. Lymphocyte migration through cultured endothelial cell monolayers derived from the blood-retinal barrier. Immunology. 80:1993;401-406.
19. Greenwood J., Wang Y., Calder V. Lymphocyte adhesion and transendothelial migration in the CNS: The role of LFA-1, ICAM-1, VLA-4 and VCAM-1. Immunology. 86:1995;408-415.
20. Hughes C.C.W., Male D.K., Lantos P.L. Adhesion of lymphocytes to cerebral microvascular cells: Effects of interferon-γ, tumour necrosis factor and interleukin-1. Immunology. 64:1988;677-681.
21. Jat P.S., Cepko C.L., Mulligan R.C., Sharp P.A. Recombinant retroviruses encoding simian virus 40 large T antigen and polyomavirus large and middle T antigens. Mol. Cell. Biol. 6:1986;1204-1217.
22. Joó, F. (1996) Isolated brain microvessels and cultured cerebral endothelial cells in blood-brain barrier research: 20 years on. In: J. Greenwood, D. Begley and M. Segal (Eds.), New Concepts of a Blood-Brain Barrier. Plenum, New York, pp. 229-237.
23. Lal B., Indurti R.R., Couraud P-O., Goldstein G.W., Laterra J. Endothelial cell implantation and survival within experimental gliomas. Proc. Natl. Acad. Sci. USA. 91:1994;9695-9699.
24. Litchfield T., Whiteley S.J.O., Greenwood J., Adamson P., Lund R.D. Grafting SV40 transformed rat retinal epithelial cells to the sub-retinal space. Invest. Ophthalmol. Vis. Sci. 37:1996;S93.
25. Male D., Pryce G., Hughes C., Lantos P. Lymphocyte migration into brain modelled in vitro: Control by lymphocyte activation, cytokines, and antigen. Cell. Immunol. 127:1990;1-11.
26. Male D., Rahman J., Pryce G., Tamatani T., Miyasaka M. Lymphocyte migration into the CNS modelled in vitro: Roles of LFA-1, ICAM-1 and VLA-4. Immunology. 81:1994;366-372.
27. Male D., Rahman J., Linke A., Zhao W., Hickey W. An interferon-inducible molecule on brain endothelium which controls lymphocyte adhesion mediated by integrins. Immunology. 84:1995;453-460.
28. McCarron R.M., Kempski O., Spatz M., McFarlin D. Presentation of myelin basic protein by murine cerebral vascular endothelial cells. J. Immunol. 134:1985;3100-3103.
29. McGuire P.G., Orkin R.W. Isolation of rat aortic endothelial cells by primary explant techniques and their phenotypic modulation by defined substrata. Lab. Invest. 57:1987;94-105.
30. McLaren M.J., Sasabe T., Li C.Y., Brown M.E., Inana G. Spontaneously arising immortal cell line of rat retinal pigmented epithelial cells. Exp. Cell Res. 204:1993;311-320.
31. Neill J.M., Barnstable C.J. Expression of the cell surface antigens RET-PE2 and N-CAM by rat retinal pigment epithelial cells during development and in tissue culture. Exp. Eye Res. 51:1990;573-583.
32. Nabi I.R., Mathews A.P., Cohen-Gould L., Gundersen D., Rodriguez-Boulan E. Immortalization of polarized retinal pigment epithelium. J. Cell Sci. 104:1993;37-49.
33. Risau W., Engelhardt B., Wekerle H. Immune function of the blood-brain barrier: Incomplete presentation of protein (auto-) antigens by rat brain microvascular endothelium in vitro. J. Cell Biol. 110:1990;1757-1766.
34. Roux F., Durieu-Trautmann O., Chaverot N., Claire M., Mailly P., Bourre J.M., Strosberg A.D., Couraud P-O. Regulation of gamma-glutamyl-transpeptidase and alkaline phosphatase activities in immortalised rat brain microvessel endothelial cells. J. Cell. Physiol. 159:1994;101-113.
35. + T cell clones in the rat. J. Immunol. Meth. 121:1989;185-196.
36. Towler, H.M.A., Greenwood, J. and Butt, A.M. (1994) Electrical resistance across the inner blood-retinal barrier in the intact retina of the anaesthetised Lewis rat. J. Physiol. 480, 10-11P.
37. Wang Y., Greenwood J., Calder V., Lightman S. Lymphocyte adhesion to cultured endothelial cells of the blood-retinal barrier. J. Neuroimmunol. 48:1993;161-168.
38. Wang Y., Calder V.L., Lightman S.L., Greenwood J. Antigen presentation by rat brain and retinal endothelial cells. J. Neuroimmunol. 61:1995;231-239.
39. + T cell lines with antigen and mitogen. Cell. Immunol. 159:1994;220-234.