A rapid viability assay for plant shoot apical meristems

Russian Journal of Plant Physiology

Volumn 43, Issue 2, 1996, Pages 263-269

  Popov, A S ^a   Vysotskaya, O N ^a  

^a TIMIRYAZEV INSTITUTE OF PLANT PHYSIOLOGY   (Russian Federation)

Author keywords

Apices; Fluorescein diacetate; Viability

Indexed keywords

EID: 0030537088     PISSN: 10214437     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Thuraton, K.C., Spencer, S.J., and Arditti, J., Phytotoxicity of Fungicides and Bactericides in Orchid Culture Media, Am. J. Bot., 1979, vol. 66, no. 7, p. 825.
2. Blekhtsin, I.Ya. and Rafikov, S.A., Estimation of Social and Economical Effectiveness of the Protection of Plants and Plant Communities against Environmental Pollution, in Problemy fitogigieny i okhrana okruzhayushchei sredy (Problems of Phytohygiene and Environmental Protection), Leningrad: Nauka, 1981. p. 75.
3. Burholt, D.R. and van't Hof, J., Cell Population Kinetics of Pisum Root Meristem Cells during and after a Mitotic-Inhibitory Exposure to Protracted y-Irradiation, Intern. J. Radiat. Biol., 1972, vol. 21, no. 4, p. 307.
4. Manzhulin, A. V., Sposoby sokhraneniya genofonda sortov i vidov kartofelya (Methods for the Conservation of the Germplasm of Potato Cultivars and Species), Pushchino: Otd. Nauchno-Tekh. Inform., Nauchn. Tsentr. Biol. Issl., Akad. Nauk SSSR, 1986.
5. Grout, B.W.W. and Henshaw, G.G., Structural Observations on the Growth of Potato Shoot-Tip Cultures after Thawing from Liquid Nitrogen, Ann. Bot. (London), 1980, vol. 46, no. 2, p. 243.
6. Haskins, R.H. and Kartha, K.K., Freeze Preservation of Pea Meristems: Cell Survival, Can. J. Bot., 1980, vol. 58, no. 8, p. 833.
7. Diettrich, B., Wolf, T., Bormann, A., Popov, A.S., Butenko, R.G., and Luckner, M., Cryopreservation of Digitalis lanata Shoot Tips, Planta Med., 1987, vol. 53, no. 4, p. 359.
8. Popov, A.S., Volkova, L.A., Gonzalez, T.M.A., Diettrich, B., Donat, P., Donets, N.V., and Chernyak, N.D., Cryopreservation of Plant Cell Strains and Meristems: Effects of Pretreatment and Cryoprotectants, in Biologiya kul'tiviruemykh kletok i biotekhnologiya rastenii (Biology of Plant Cell Cultures and Plant Biotechnology), Moscow: Nauka, 1991, p. 260.
9. Gukasyan, I.A., Butenko, R.G., Petoyan, S.A., and Sevost'yanova, T.A., Morphogenesis of Excised Apices of Everbearing Pink on Artificial Medium, Fiziol. Rast. (Moscow), 1977, vol. 24, no. 1, p. 168 (Sov. Plant Physiol., Engl. Transl.).
10. Manzhulin, A.V., Factors Affecting Potato Stem Apex Survival after Deep Freezing, Fiziol. Rast. (Moscow), 1984, vol. 31, no. 4, p. 639 (Sov. Plant Physiol., Engl. Transl.).
11. Bajaj, Y.P.S., Clonal Multiplication and Cryopreservation of Cassava through Tissue Culture, Crop Improv., 1977, vol. 4, no. 2, p. 198.
12. Kartha, K.K., Leung, N.L., and Pahl, K., Cryopreservation of Strawberry Meristems and Mass Propagation of Plantlets, J. Am. Soc. Hortic. Sci., 1980, vol. 105, no. 4, p. 481.
13. Kartha, K.K., Leung, N.L., and Mroginskii, L.A., In vitro Growth Responses and Plant Regeneration from Cryopreserved Meristems of Cassava (Manichot esculenta Crantz.), Intern. J. Plant Physiol., 1982, vol. 107, no. 2, p. 133.
14. Towill, L.E., Improved Survival after Cryogenic Exposure of Shoot Tips Derived from in vitro Plantlet Culture of Potato, Cryobiology, 1983, vol. 20, no. 5, p. 567.
15. Steponkus, P.L. and Lanphear, F.O., Refinement of the Triphenyl Tetrazolium Chloride Method of Determining Cold Injury, Plant Physiol., 1967, vol. 42, no. 10, p. 1423.
16. Widholm, J.M., The Use of Fluorescein Diacetate and Phenosafranine for Determining Viability of Cultured Plant Cells, Stain Technol., 1972, vol. 47, no. 4, p. 189.
17. Towill, L.E. and Mazur, P., Studies on the Reduction of 2,3,5-Triphenyl Tetrazolium Chloride as a Viability Assay for Plant Tissue Cultures, Can. J. Bot., 1974, vol. 53, no. 11, p. 1097.
18. Samygin, G.A., Volkova, L.A., and Popov, A.S., A Comparison of Different Assays for Determining Cell Viability in Suspension and Callus Cultures, Fiziol. Rast. (Moscow), 1985, vol. 32, no. 4, p. 813 (Sov. Plant Physiol., Engl. Transl.).
19. Popov, A.S., Butenko, R.G., and Glukhova, I.N., Effect of the Conditions during Preculturing and Deep Freezing on the Recovery of Wild Carrot Suspension Culture, Fiziol. Rast. (Moscow), 1978, vol. 25, no. 6, p. 1227 (Sov. Plant Physiol., Engl. Transl.).
20. Pearse, A.G. E., Histochemistry, London: Churchill Livingstone, 1960. Translated under the title Gistokhimiya, Moscow: Inostr. Lit., 1962.
21. Benson, E.E. and Hamill, J.D., Cryopreservation and Post-Freeze Molecular and Biosynthetic Stability in Transformed Roots of Beta vulgaris and Nicotiana rustica, Plant Cell, Tissue, Organ Cult., 1991, vol. 24, no. 3, p. 163.
22. Murashige, T. and Skoog, F.A., A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures, Physiol. Plant., 1962, vol. 15, no. 4, p. 473.
23. Vysotskaya, O.N., Long-Term Conservation of Strawberry Plant Collection in vitro, Fiziol. Rast. (Moscow), 1994, vol. 41, no. 6, p. 935 (Russ. J. Plant Physiol., Engl. Transl.).
24. Kaul, B. and Staba, E.J., Dioscorea Tissue Cultures: Biosynthesis and Isolation of Diosgenin from Dioscorea deltoidea Callus and Suspension Cells, Lloydia, 1968, vol. 31, no. 2, p. 171.
25. Withers, L.A. and Davey, M.K., A Fine-Structural Study of the Freeze-Preservation of Plant Tissue Cultures, Protoplasma, 1978, vol. 94, nos. 3/4, p. 207.