A rapid and simple nested PCR assay for the detection of Pneumocystis carinii in sputum samples

Scandinavian Journal of Infectious Diseases

Volumn 28, Issue 6, 1996, Pages 597-600

  Olsson, Mats ^a,c   Elvin, Kerstin ^b   Lidman, Christer ^b   Löfdahl, Sven ^a   Linder, Ewert ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^c SWEDISH INSTITUTE FOR INFECTIOUS DISEASE CONTROL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CHLOROFORM; PHENOL; THYMIDYLATE SYNTHASE;

ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; EXTRACTION; GENE; HUMAN; HYBRIDIZATION; IMMUNOCYTOCHEMISTRY; INTERMETHOD COMPARISON; MALE; MICROSCOPY; PNEUMOCYSTIS CARINII; PNEUMOCYSTIS PNEUMONIA; POLYMERASE CHAIN REACTION; SPUTUM ANALYSIS;

EID: 0030514464     PISSN: 00365548     EISSN: None     Source Type: Journal    
DOI: 10.3109/00365549609037967     Document Type: Article

References (22)

1. Eriksson O. Pneumocystis carinii, a parasite in lungs of mammals, referred to a new family and order (Pneumocystidaceae, Pneumocystidales, Ascomycota). Systema Ascomycetum 13: 165-180, 1994.
2. Edman U, Edman JC, Lundgren B, Santi D. Isolation and expression of the Pneumocystis carinii thymidylate synthase gene. Proc Natl Acad Sci USA 86: 6503-6507, 1989.
3. Olsson M, Elvin K, Löfdahl S, Linder E. Detection of Pneumocystis carinii DNA in sputum and bronchoalveolar lavage samples by polymerase chain reaction. J Clin Microbiol 31: 221-226, 1993.
4. Ho-Yen DO, Chatterton JMW, Joss AW. Laboratory diagnosis of Pneumocystis carinii pneumonia. J Med Microbiol 42: 231-232, 1995.
5. Lipschik GY, Gill VJ, Lundgren JD, Andrawis VA, Nelson NA, Nielsen JO, Ognibene FP, Kovacs JA. Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood. Lancet 340: 203-206, 1992.
6. Tamburrini E, Mencarini P, Luca AD, Antinori A, Visconti E, Ammassari A, Ortona L, Ortona E, Siracusano A, Vicari G. Simple and rapid two-step polymerase chain reaction for diagnosis of Pneumocystis carinii. J Clin Microbiol 31: 2788-2789, 1993.
7. Moonens F, Liesnard C, Brancart F, Vooren JPv, Serruys E. Rapid simple and nested polymerase chain reaction for the diagnosis of Pneumocystics carinii pneumonia. Scand J Infect Dis 27: 358-362, 1995.
8. Östergaard L, Tarp B, Jensen BN, Henriques U, Birkeland S, Christiansen G, Andersen PL. Semi-nested polymerase chain reaction for detection of Pneumocystis carinii: implications for diagnosis, prevalence and predictive parameters. Immunology and Infectious Diseases 5: 59-66, 1995.
9. Sköt J, Lerche AX, Kolmos HJ, Nielsen JO, Mathiesen LR, Lundgren JD. Pneumocystis carinii in bronchoalveolar lavage and induced sputum: detection with a nested polymerase chain reaction. Scand J Infect Dis 27: 363-367, 1995.
10. Levine S, Masur H, Vee J, Feuerstein I, Suffredini A, Brown D, Lane H, Yarchoan R, Shelhamer J, Ognibene F. Effect of aerosolized pentamidine prophylaxis on the diagnosis of Pneumocystis carinii pneumonia by induced sputum examination in patients infected with human immunodefiency virus. Am Rev Respir Dis 144: 760-764, 1991.
11. Elvin K, Björkman A, Linder E, Heurlin N, Hjerpe A. Pneumocystis carinii pneumonia: detection of parasites in sputum and bronchoalveolar lavage fluid by monoclonal antibodies. Br Med J 297: 381-384, 1988.
12. Tiley S, Marriott D, Harkness J. An evaluation of four methods for the detection of Pneumocystis carinii in clinical specimen. Pathology 26: 325-328, 1994.
13. Elvin K, Lidman C, Tynell E, Linder E, Björkman A. Natural history of asymptomatic and symptomatic Pneumocystis carinii infection in HIV patients. Scand J Infect Dis 26: 643-651, 1994.
14. Lidman C, Berglund O, Tynell E, Lindbäck S, Elvin K. Aerosolized pentamidine as primary prophylaxis for Pneumocystis carinii pneumonia: efficacy, mortality and morbidity. AIDS 8: 935-939, 1994.
15. Linder E, Lundin L, Vorma H. Detection of Pneumocystis carinii in lung-derived samples using monoclonal antibodies to an 82 kDa parasite component. J Immunol Meth 98: 57-62, 1987.
16. Wilson K. Preparation of genomic DNA from bacteria. In: Ausabel FM, Brent R, Kingston RE, Moore DD, Smith JA, Seidman JG, Struhl K, eds. Current protocols in molecular microbiology. New York: Greene Publishing and Wiley Interscience, 2.4.1-2.4.5, 1988.
17. Chatterton JMW, Joss AWL, Davidson MM, Ho-Yen DO. Why have Pneumocystis carinii trophozoites been ignored? J Clin Pathol 43: 265-268, 1990.
18. Leigh T, Kangro H, Gazzard B, Jeffries D, Collins J. DNA amplification by the polymerase chain reaction to detect sub-clinical Pneumocystis carinii colonization in HIV-positive and HIV-negative male homosexuals with and without respiratory symptoms. Respir Med 87: 525-529, 1993.
19. Wakefield AE, Guiver L, Miller RF, Hopkin JM. DNA amplification on induced sputum samples for diagnosis of Pneumocystis carinii pneumonia. Lancet 337: 1378-1379, 1991.
20. Roux P, Poirot JL, Chouaid C, Denis M, Olivier JI, Nigou M, Miltgen M. Usefulness of PCR for detection of Pneumocystis carinii DNA. J Clin Microbiol 32: 2324-2326, 1994.
21. Leibovitz E, Pollack H, Moore T, Papellas J, Gallo L, Krasinski K, Borkowsky W. Comparison of PCR and standard cytological staining for detection of Pneumocystis carinii from respiratory specimen from patients with or at high risk for infection by human immunodefiency virus. J Clin Microbiol 33: 3004-3007, 1995.
22. Elvin K, Linder E. Application and staining pattern of commercial anti-Pneumocystis carinii monoclonal antibodies. J Clin Microbiol 31: 2222-2224, 1993.