Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen : Differences in response between populations

Fundamental and Applied Nematology

Volumn 19, Issue 3, 1996, Pages 227-234

  Van Der Beek, Hans J G ^a   Veldhuis, Wim B J ^a   Zijlstra, Carolien ^a   Van Silfhout, Cor H ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Cryopreservation; Cryoprotectant; Ethanediol; Freezing; Infectivity; Lipid staining; Root knot nematode; Survival

Indexed keywords

MELOIDOGYNE CHITWOODI; MELOIDOGYNE HAPLA;

EID: 0030512555     PISSN: 11645571     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. BEEN, T. H., MEIJER, E. M. J., BENIER, J. E. & KNOL, J. B. (1996). Using image analysis for counting larvae of potato cyst nematodes (Globodera spp.). Fundam. appl. Nematol., 19 : 297-304.
2. BOUKEMA, I. W., HOFMAN, K., TOXOPEUS, H. & LAMERS, F. W. M. (1984). An efficient screening method for root knot nematode resistance in cucurbits and crucifers. In : Lange, W., Zeven A. C. & Hoogenboom, N. G. (Eds). Efficiency in plant breeding. Wageningen, The Netherlands, Pudoc : 299.
3. BRIDGE, J. & HAM, J. (1985). A technique for the cryopreservation of viable juveniles of Meloidogyne graminicola. Nematologica, 31 : 185-189.
4. CURRAN, J., GILBERT, C. & BUTLER, K. (1992). Routine cryopreservation of isolates of Steinernema and Heterorhabditis spp. J. Nematol., 24 : 269-270.
5. DE CONINCK, L. A. P. (1951). On the resistance of the free-living nematode Anguillula silusiae to low temperatures. Biodynamica, 7 : 77-84.
6. ESBENSHADE, P. R. & TRIANTAPHYLLOU, A. C. (1990). Isozyme phenotypes for the identification of Meloidogyne species. J. Nematol., 22 : 10-15.
7. GEHINIO, P. M. & LUYET, B. J. (1951). Effect of a preliminary slight dehydration on the survival of "vinegar eels" frozen at - 77 C. Biodynamica, 7 : 42-52.
8. HAIGHT, M., FRIM, J., PASTERNAK, J. & FREY, H. (1975). Freeze-thaw survival of the free-living nematode Caenorhabditis briggsae. Cryobiology, 12 : 497-505.
9. HAM, P. J., TOWNSON, S., JAMES, E. R. & BIANCO, A. E. (1981). An improved technique for the cryopreservation of Onchocerca microfilariae. Parasitology, 83 : 139-146.
10. HWANG, S. W. (1970). Freezing and storage of nematodes in liquid nitrogen. Nematologica, 16 : 305-308.
11. JAMES, E. R. (1981). Schistosoma mansoni: Cryopreservation of Schistosomula by two-step addition of ethanediol and rapid cooling. Expl Parasit., 52 : 105-116.
12. PAYNE, R. W., LANE, P. W., AINSLEY, A. E., BICKNELL, K. E., DIGBY, P. G. N., HARDING, S. A., LEECH, P. K., SIMPSON, H. R., TODD, A. D., VERRIER, P. J., WHITE, R. P., GOWER, J. C., TUNNICLIFFE WlLSON, G., PATERSON, L. J. (1989). Genstat 5 reference manual. Oxford, UK, Clarendon Press, 749 p.
13. POPIEL, I. & VASQUEZ, E. M. (1991). Cryopreservation of Steinernema carpocapsae and Heterorhabditis bacteriophora. J. Nematol., 23 : 432-437.
14. RAHM, P. G. (1921). Einwirkung sehr niederer Temperaturen auf die Moosfauna. Proc. Sect. Sci. K. Akad. Wetensch., Amsterdam, 23 : 235-248.
15. RAHM, P. G. (1922). Weitere physiologische Versuche mit niederen Temperaturen. Proc. Sect. Sci. K. Akad. Wetensch., Amsterdam, 24 : 192-194.
16. RIGA, E & WEBSTER, J. M. (1991). Cryopreservation of the pinewood nematode, Bursaphelenchus spp. J. Nematol., 23 : 438-440.
17. SAYRE, R. M. & HWANG, S. W. (1975). Freezing and storing Ditylenchus dipsaci in liquid nitrogen. J. Nematol., 7 : 199-202.
18. SMITH, B. S., HODGSON-SMITH, A., POPIEL, I., MINTER, D. M. & JAMES, E. R. (1990). Cryopreservation of the entomogenous nematode parasite Steinernema feltiae (= Neoaplectana carpocapsae). Cryobiology, 27 : 319-327.
19. STOREY, R. M. J. (1984). The relationship between neutral lipid reserves and infectivity for hatched and dormant juveniles of Globodera spp. Ann. appl. Biol., 104 : 511-520.
20. TRIANTAPHYLLOU, A. C. & MCCABE, E. (1989). Efficient preservation of root-knot and cyst nematodes in liquid nitrogen. J. Nematol., 21 : 423-426.
21. VAN MECHELEN, J. C., KARSSEN, G., JANSSEN, G. J. W., VERKERK-BAKKER, B., JANSSEN, R. (1994). A new race of Meloidogyne chitwoodi Golden, O'Bannon, Santo & Finley, 1980? Fundam. appl. Nematol., 17 : 93-96.
22. WOMERSLEY, C., THOMPSON, S. N. & SMITH, L. (1982). Anhydrobiosis in nematodes II : Carbohydrate and lipid analysis in undesiccated and desiccated nematodes. J. Nematol., 14 : 145-153.