Reduction in porphyrin excretion as a sensitive indicator of lead toxicity in primary cultures of adult rat hepatocytes

Toxicology in Vitro

Volumn 10, Issue 6, 1996, Pages 675-683

  Quintanilla Vega, B ^a   Hernandez, A ^a   Mendoza Figueroa, T ^a  

^a DEPARTAMENTO DE FÍSICA   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

COPROPORPHYRIN; LEAD; PORPHOBILINOGEN SYNTHASE; PORPHYRIN; PROTOPORPHYRIN; UROPORPHYRIN; UROPORPHYRINOGEN DECARBOXYLASE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOTOXICITY; EXCRETION; HEME SYNTHESIS; LEAD POISONING; LIVER CELL; MALE; MEASUREMENT; NONHUMAN; PORPHYRIN METABOLISM; RAT;

ANIMALIA;

EID: 0030499871     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0887-2333(96)00043-4     Document Type: Article

References (26)

1. Bernard A. and Lauwerys R. (1987) Metal-induced alterations of 5-aminolevulinic acid dehydratase. Annals of the New York Academy of Science 514, 41-47.
2. Brady A. M. and Lock E. A. (1992) Inhibition of ferrochelatase and accumulation of porphyrins in mouse hepatocyte cultures exposed to porphyrinogenic chemicals. Archives of Toxicology 66, 175-181.
3. Cable E. E., Pepe J. A., Karamitsios N. C., Lambrecht R. W. and Bonkovsky H. L. (1994) Differential effects of metalloporphyrins on messenger RNA levels of delta-aminolevulinate synthase and haem oxygenase. Studies in cultured chick embryo liver cells. Journal of Clinical Investigation 94, 649-654.
4. Colombo J. P. and Konarska L. (1983) Arginase. In Methods of Enzymatic Analysis. Edited by J. Bergmeyer and M. Grable. pp. 285-294. Verlag Chemie, Weinheim.
5. DelRaso N. J. (1992) In vitro methods for assessing chemical or drug toxicity and metabolism in primary hepatocytes. In In Vitro Methods of Toxicology. Edited by R. R. Watson. pp. 175-201. CRC Press, Boca Raton, FL.
6. Denizeau F., Marion M., Chtaib M. and Schmit J. P. (1990) Toxicity of heavy metals in cultured hepatocytes. Environmental Toxicology and Chemistry 9, 737-743.
7. ECVAM (1994) Workshop 1. The practical applicability of hepatocyte cultures in routine testing. ATLA 22, 231-241.
8. Evarts R. P., Marsden E. and Thorgeirsson S. S. (1984) Regulation of heme metabolism and cytochrome P-450 levels in primary culture of rat hepatocytes in a defined medium. Biochemical Pharmacology 33, 565-569.
9. Fowler B. A., Kimmel C. A., Woods J. S., McConnell E. E. and Grant L. D. (1980) Chronic low-level lead toxicity in the rat. III. An integrated toxicological assessment with special reference to the kidney. Toxicology and Applied Pharmacology 56, 59-77.
10. Grandchamp B., Deybach J. C., Grelier M., De Verneuil H. and Nordmann Y. (1980) Studies of porphyrin synthesis in fibroblasts of patients with congenital erythropoietic porphyria and one patient with homozygous coproporphyria. Biochimica et Biophysica Acta 629, 577-586.
11. Hamilton J. W., Bernent W. J., Sinclair P. R., Sinclair J. F. and Watterhahn K. E. (1988) Expression of 5-aminolevulinate synthase and cytochrome P-450 mRNAs in chicken embryo hepatocytes in vivo and in culture. Effect of porphyrinogenic drugs and haem. Biochemical Journal 225, 267-275.
12. Hamilton J. W., Bement W. J., Sinclair P. R., Sinclair J. F., Alcedo J. A. and Watterhahn K. E. (1991) Haem regulates hepatic 5-aminolevulinate synthase mRNA expression by decreasing mRNA half-life and not by altering its rate of transcription. Archives of Biochemistry and Biophysics 289, 387-392.
13. Klaassen C. D. and Stacey N. H. (1987) Metals, hepatocytes, and toxicology. In The Isolated Hepatocyte: Use in Toxicology and Xenobiotic Biotransformations. Edited by E. J. Rauckman and G. M. Padilla. pp. 159-187. Academic Press, London.
14. Kuri-Harcuch W. and Mendoza-Figueroa T. (1989) Cultivation of adult rat hepatocytes on 3T3 cells. Expression of various liver differentiated functions. Differentiation 41, 148-157.
15. Lachapelle M., Guertin F., Marion M., Fournier M. and Denizeau F. (1993) Mercuric chloride affects protein secretion in rat primary hepatocyte cultures: a biochemical ultrastructural, and gold immunocytochemical study. Journal of Toxicology and Environmental Health 38, 343-354.
16. Lowry O. H., Rosebrough N. J., Farr A. L. and Randall R. J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265-275.
17. Marks G. S. (1985) Exposure to toxic agents: the heme biosynthetic pathway and hemoproteins as indicators. CRC Critical Reviews in Toxicology 15, 151-179.
18. Marks G. S., Powles J., Lyon M., McCluskey S., Sutherland E. and Zelt D. (1987) Patterns of porphyrin accumulation in response to xenobiotics. Parallels between results in chick embryo and rodents. Annals of the New York Academy of Science 514, 113-122.
19. Quintanilla-Vega B., Hernández A., López M. L., García-Vargas G., Cebrián M. E. and Mendoza-Figueroa T. (1995) Porphyrin production and excretion by long-term cultures of adult rat hepatocytes and effect of lead exposure. Toxicology 102, 275-283.
20. Reitman S. and Frankel P. (1957) A colorimetric method for the determination of serum oxaloacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology 28, 56-63.
21. Sierra-Santoyo A., López M. L., Hernández A. and Mendoza-Figueroa T. (1994) Urea production in long-term cultures of adult rat hepatocytes. Toxicology in Vitro 8, 293-299.
22. Straka J. G. and Kushner J. P. (1983) Purification and characterization of bovine hepatic uroporphyrinogen decarboxylase. Biochemistry 22, 4664-4672.
23. Sinclair P. R., Bernent W. J., Bonkovsky H. L. and Sinclair J. F. (1984) Inhibition of uroporphyrinogen decarboxylase by halogenated biphenyls in chick hepatocyte cultures. Biochemical Journal 222, 737-748.
24. Sinclair P. R., Bement W. J., Gorman N., Liem H. H., Wolkoff A. W. and Muller-Eberhard U. (1988) Effects of serum proteins on haem uptake and metabolism in primary cultures of liver cells. Biochemical Journal 256, 159-265.
25. Woods J. S. and Fowler B. A. (1987) Metal alterations of uroporphyrinogen decarboxylase and coproporphyrinogen oxidase. Annals of the New York Academy of Science 514, 55-64.
26. Wyckoff E. E. and Kushner J. P. (1994) Haem biosynthesis, the porphyrias, and the liver. In The Liver: Biology and Pathobiology. Edited by I. M. Arias, J. L. Boyer, N. Fausto, W. B. Jacoby, D. A. Schachterand and D. A. Shafritz. pp. 505-527. Raven Press, New York.