Microwave-antigen retrieval: The importance of pH of the retrieval solution for MiB-1 staining

European Journal of Morphology

Volumn 34, Issue 5, 1996, Pages 375-379

  Boon, Mathilde E ^a  

^a LEIDEN CYTOLOGY AND PATHOLOGY LABORATORY   (Netherlands)

Author keywords

antigen retrieval; MiB 1; microwaves; proliferation markers

Indexed keywords

ANTIGEN;

ARTICLE; CELL PROLIFERATION; HISTOLOGY; MICROSCOPY; MICROWAVE RADIATION; PRIORITY JOURNAL; STAINING; TEMPERATURE; TISSUE INJURY;

ANTIBODIES, MONOCLONAL; ANTIGEN-ANTIBODY REACTIONS; ANTIGENS, NUCLEAR; AUTOANTIGENS; CELL NUCLEUS; HEAT; HYDROGEN-ION CONCENTRATION; KI-67 ANTIGEN; MICROWAVES; NUCLEAR PROTEINS; STAINING AND LABELING; TISSUE FIXATION;

EID: 0030482968     PISSN: 09243860     EISSN: None     Source Type: Journal    
DOI: 10.1076/ejom.34.5.375.13053     Document Type: Article

References (13)

1. BOON ME, KLEINSCHMIDT-GUY ED, OUWERKERKNOORDAM E (1994) PAPNET for analysis of proliferating (MiB-1 positive) cell populations in cervical smears. Eur J Morphol 32, 78-85
2. CATTORETTI G, BECKER MHG, KEY G, DUCHROW M, SCHLÜTER C, GALLE J, GERDES J (1992) Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol 168, 357-363
3. DOOKHAM DB, KOVATICH AJ, MIETTINEN M (1993) Non-enzymatic antigen retrieval in immunohistochemistry. Comparison between different antigen retrieval modalities and proteolytic digestion. Appl Immunohistochem 1, 149-155
4. EMANUELS A, HOLLEMA H, SUURMEIJER A, KOUDSTAAL J (1994) A modified method for antigen retrieval MiB-1 staining of vulvar carcinoma. Eur J Morphol 32, 335-337
5. FRAENKEL-CONRAT H, BRANDON BA, OLCOTT HS (1947) The reaction of formaldehyde with proteins. IV. Participation of indole groups. Gramicidin J Biol Chem 168, 99-118
6. HOPWOOD D (1994) Epitope retrieval - survey and prospect. Eur J Morphol 32, 317-324
7. MASON JT, O'LEARY TJ (1991) Effect of formaldehyde fixation on protein secondary structure: a calimetric and infrared spectoscopic investigation. J Histochem Cytochem 39, 225
8. SHI SR, IMAM SA, YOUNG L, COTE RJ, TAYLOR CR (1995) Antigen retrieval immunohistochemistry under the influence of pH using monoclonal antibodies. J Histochem Cytochem 43, 193-201
9. SHI SR, KEY ME, KALRA KL (1991) Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 39, 741-748
10. SHIN RW, IWAKI T, KIKAMOTO T, TAEISHI J (1991) Hydrated autoclave pretreatment enhances tau immunoreactivity in formalin- fixed normal and Alzheimer's disease brain tissues. Lab Invest 64, 693-702
11. SUURMEIJER AJH (1994) Optimizing immunohistochemistry in diagnostic tumor pathology with antigen retrieval. Eur J Morphol 32, 325-330
12. VAN DEN BROEK LJCM, HAZELBAG HM, HILDERS CGJM, FLEUREN GJ (1994) Antigen retrieval and microwaving. Editorial. Eur J Morphol 32, 65-70
13. VAN DER POEL HG, BOON ME (1994) Microwave-antigen retrieval for proliferation analysis (MiB-1) and quanticyt karyometry of bladder washings. Eur J Morphol 32, 71-78