α-Tubulin limits its own synthesis: Evidence for a mechanism involving translational repression

Journal of Cell Biology

Volumn 135, Issue 6, 1996, Pages 1525-1534

  Gonzalez Garay, Manuel L ^a   Cabrai, Fernando ^a  

^a UNIVERSITY OF TEXAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA TUBULIN; COMPLEMENTARY DNA;

ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; GENE REPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS INHIBITION; TRANSLATION REGULATION;

AMINO ACID SEQUENCE; ANIMALS; CHO CELLS; CLONING, MOLECULAR; COLCHICINE; CRICETINAE; CRICETULUS; GENE EXPRESSION REGULATION; HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; MOLECULAR SEQUENCE DATA; PROTEIN BIOSYNTHESIS; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; TRANSFECTION; TUBULIN;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; MAMMALIA;

EID: 0030481488     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.135.6.1525     Document Type: Article

References (32)

1. Barlow, S.B., M.L. Gonzalez-Garay, R.R. West, J.B. Olmsted, and F. Cabral. 1994. Stable expression of heterologous microtubule associated proteins in Chinese hamster ovary cells: evidence for differing roles of MAPs in microtubule organization. J. Cell Biol. 126:1017-1029.
2. Boggs, B., and F. Cabral. 1987. Mutations affecting assembly and stability of tubulin: evidence for a non-essential β-tubulin in CHO cells. Mol. Cell. Biol. 7: 2700-2707.
3. Burke, D., P. Gasdaska, and L. Hartwell. 1989. Dominant effects of tubulin overexpression in Saccharomyces cerevisiae. Mol. Cell. Biol. 9:1049-1059.
4. Cabrai, F., and M.M. Gottesman. 1978. The determination of similarities in amino acid composition among proteins separated by two-dimensional gel electrophoresis. Anal. Biochem. 91:548-556.
5. Cabrai, F., and G. Schatz. 1979. One-and two-dimensional electrophoretic analysis of mitochondrial membrane proteins. Methods Enzymol. 56:602-613.
6. Chen, C., and H. Okayama. 1987. High-efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7:2745-2752.
7. Chu, E., D.M. Koeller, J.L. Casey, J.C. Drake, B.A. Chabner, P.C. Elwood, S. Zinn, and C.J. Allegra. 1991. Autoregulation of human thymidylate synthase messenger RNA translation by thymidylate synthase. Proc. Natl. Acad. Sci. USA. 88:8977-8981.
8. Chu, E., C.H. Takimoto, D. Voeller, J.L. Grem, and C.J. Allegra. 1993. Specific binding of human dihydrofolate reductase protein to dihydrofolate reductase messenger RNA in vitro. Biochemistry. 32:4756-4760.
9. Cleveland, D.W. 1989. Autoregulated control of tubulin synthesis in animal cells. Curr. Opin. Cell Biol. 1:10-14.
10. Dhamodharan, R., M.A. Jordan, D. Thrower, L. Wilson, and P. Wadsworth, 1995. Vinblastine suppresses dynamics of individual microtubules in living interphase cells. Mol. Biol. Cell. 6:1215-1229.
11. Elliott, E.M., G. Henderson, F. Sarangi, and V. Ling. 1986. Complete sequence of three α-tubulin cDNAs in Chinese hamster ovary cells: each encodes a distinct α-tubulin isoprotein. Mol. Cell. Biol. 6:906-913.
12. Gonzalez-Garay, M.L., and F. Cabral. 1995. Overexpression of an epitopetagged β-tubulin in Chinese hamster ovary cells causes an increase in endogenous α-tubulin synthesis. Cell Motil. Cytoskeleton. 31:259-272.
13. Gorman, C.M., and B.H. Howard. 1983. Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate. Nucleic Acids Res. 11: 7631-7648.
14. Hershey, J.W.B. 1991. Translational control in mammalian cells. Annu. Rev. Biochem. 60:717-755.
15. Jordan, M.A., R.J. Toso, D. Thrower, and L. Wilson. 1993. Mechanism of mitotic block and inhibition of cell proliferation by taxol at low concentrations. Proc. Natl. Acad. Sci. USA. 90:9552-9556.
16. Katz, W., B. Weinstein, and F. Solomon. 1990. Regulation of tubulin levels and microtubule assembly in Saccharomyces cerevisiae: consequences of altered tubulin gene copy number. Mol. Cell. Biol. 10:5286-5292.
17. Kemphues, K.J., T.C. Kaufman, R.A. Raff, and E.C. Raff. 1982. The testis-specific β-tubulin subunit in Drosophila melanogaster has multiple functions in spermatogenesis. Cell. 31:655-670.
18. Klausner, R.D., T.A. Rouault, and J.B. Harford. 1993. Regulating the fate of mRNA: the control of cellular iron metabolism. Cell. 72:19-28.
19. Lindahl, L., and A. Hinnebusch. 1992. Diversity of mechanisms in the regulation of translation in prokaryotes and lower eukaryotes. Curr. Opin. Genet. Dev. 2:720-726.
20. May, G.S., R.B. Waring, and N.R. Morris. 1990. Increasing tubC beta-tubulin synthesis by placing it under the control of a benA beta-tubulin upstream sequence causes a reduction in benA beta-tubulin level but has no effect on microtubule function. Cell Motil. Cytoskeleton. 16:214-220.
21. Minotti, A.M., S.B. Barlow, and F. Cabral. 1991. Resistance to antimitotic drugs in Chinese hamster ovary cells correlates with changes in the level of polymerized tubulin. J. Biol. Chem. 266:3987-3994.
22. Nagy, L., V.A. Thomazy, G.L. Shipley, L. Fesus, W. Lamph, R.A. Heyman, R.A.S. Chandraratna, and P.J.A. Davies. 1995. Activation of retinoid X receptors induces apoptosis in HL-60 cell lines. Mol. Cell. Biol. 15:3540-3551.
23. Nomura, M., R. Gourse, and G. Baughman. 1984. Regulation of the synthesis of ribosomes and ribosomal components. Annu. Rev. Biochem. 53:75-117.
24. Sawada, T., and F. Cabral. 1989. Expression and function of β-tubulin isotypes in Chinese hamster ovary cells. J. Biol. Chem. 264:3013-3020.
25. Sisodia, S.S., D.A. Gay, and D.W. Cleveland. 1990. In vivo discrimination among β-tubulin isotypes: selective degradation of a type IV β-tubulin isotype following overexpression in cultured animal cells. New Biologist. 2:66-76.
26. Spiegelman, B.M., S.M. Penningroth, and M.W. Kirschner. 1977. Turnover of tubulin and the N site GTP in Chinese hamster ovary cells. Cell. 12:587-600.
27. Standart, N., and R.J. Jackson. 1994. Regulation of translation by specific protein/mRNA interactions. Biochimie (Paris). 76:867-879.
28. Sullivan, K.F. 1988. Structure and utilization of tubulin isotypes. Annu. Rev. Cell Biol. 4:687-716.
29. Theodorakis, N.G., and D.W. Cleveland. 1992. Physical evidence for cotranslational regulation of β-tubulin mRNA degradation. Mol. Cell. Biol. 12:791-799.
30. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:4350-4354.
31. Whitfield, C., I. Abraham, D. Ascherman, and M.M. Gottesman. 1986. Transfer and amplification of a mutant β-tubulin gene results in colcemid dependence: use of the transformant to demonstrate regulation of β-tubulin subunit levels by protein degradation. Mol. Cell. Biol. 6:1422-1429.
32. Yaffe, M.B., B.S. Levison, J. Szasz, and H. Sternlicht. 1988. Expression of a human α-tubulin: properties of the isolated subunit. Biochemistry. 27:1869-1880.