Mutagenesis of N-glycosylation sites in the human VIP 1 receptor

Annals of the New York Academy of Sciences

Volumn 805, Issue , 1996, Pages 558-562

  Couvineau, Alain ^a   Fabre, Catherine ^a   Gaudin, Pascale ^a   Maoret, Jean José ^a   Laburthe, Marc ^a  

^a INSERM   (France)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; MUTANT PROTEIN; RECEPTOR SUBTYPE; VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR;

AMINO TERMINAL SEQUENCE; BINDING AFFINITY; CONFERENCE PAPER; CONSENSUS SEQUENCE; DISSOCIATION CONSTANT; HUMAN; HUMAN CELL; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN SECONDARY STRUCTURE; SITE DIRECTED MUTAGENESIS;

EID: 0030481084     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.1996.tb17519.x     Document Type: Conference Paper

References (7)

1. COUVINEAU, A., C. ROUYER-FESSARD, D. DARMOUL, J. J. MAORET, I. CARRERO, E. OGIER-DENIS & M. LABURTHE. 1994. Human intestinal VIP receptor: Cloning and functional expression of two cDNA encoding proteins with different N-terminal domains. Biochem. Biophys. Res. Commun. 200: 769.
2. COUVINEAU, A., P. GAUDIN, J. J. MAORET, C. ROUYER-FESSARD, P. NICOLE & M. LABURTHE. 1995. Highly conserved aspartate 68, tryptophane 73 and glycine 109 in the N-terminal extracellular domain of the human VIP receptor are essential for its ability to bind VIP. Biochem. Biophys. Res. Commun. 206: 246-252.
3. GAUDIN, P., A. COUVINEAU, J. J. MAORET, C. ROUYER-FESSARD & M. LABURTHE. 1995. Mutational analysis of cysteine residues within the extracellular domains of the human vasoactive intestinal peptide (VIP) 1 receptor identifies seven mutants that are defective in VIP binding. Biochem. Biophys. Res. Commun. 211: 901-908.
4. NGUYEN, T. D., J. A. WILLIAMS & G. M. GRAY. 1986. Vasoactive intestinal peptide receptor on liver plasma membranes: Characterization as a glycoprotein. Biochemistry 25: 361-368.
5. EL BATTARI, A., J. LUIS, J. M. MARTIN, J. FANTINI, J. M. MULLER, J. MARVALDI & J. PICHON. 1987. The vasoactive intestinal peptide receptor on intact colonic adenocarcinoma cells (HT29-D4). Evidence for its glycoprotein nature. Biochem. J. 242: 185-191.
6. LABURTHE, M. & A. COUVINEAU. 1988. Molecular analysis of vasoactive intestinal peptide receptors. A comparison with receptors for VIP-related peptides. Ann. N.Y. Acad. Sci. 527: 296-313.
7. COUVINEAU, A., C. FABRE, P. GAUDIN, J. J. MAORET & M. LABURTHE. 1996. Mutagenesis of N-glycosylation sites in the human vasoactive intestinal peptide 1 receptor. Evidence that asparagine 58 or 69 is crucial for correct delivery of the receptor to plasma membrane. Biochemistry 35: 1745-1752.