Maize recombinant C4-pyruvate,orthophosphate dikinase: Expression in Escherichia coli, partial purification, and characterization of the phosphorylatable protein

Photosynthesis Research

Volumn 49, Issue 1, 1996, Pages 83-89

  Chastain, Chris J ^a   Thompson, Brent J ^a   Chollet, Raymond ^b  

^a MINNESOTA STATE UNIVERSITY MOORHEAD   (United States)

^b UNIVERSITY OF NEBRASKA LINCOLN   (United States)

Author keywords

C4 photosynthesis; C4 plant; maize (Zea mays); orthophosphate dikinase; PPDK; pyruvate; recombinant enzyme

Indexed keywords

ESCHERICHIA COLI; ZEA MAYS;

EID: 0030479755     PISSN: 01668595     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00029430     Document Type: Article

References (19)

1. 4 photosynthesis. In: Lea PJ (ed) Methods in Plant Biochemistry, Vol 3, pp 39-72. Academic Press, San Diego
2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254
3. Budde RJA and Chollet R (1986) In vitro phosphorylation of maize leaf phosphoenolpyruvate carboxylase. Plant Physiol 82: 1107-1114
4. i dikinase. Biochem Int 9: 683-689.
5. 4 photosynthesis: Identification of a catalytically important histidine residue and its role in the regulation of pyruvate, Pi dikinase. Arch Biochem Biophys 231: 175-182
6. 4-leaf pyruvate, orthophosphate dikinase from maize. FEBS Lett 274: 178-180
7. 4 plants: A molecular approach. Plant Cell 7: 797-807
8. 4 photosynthesis: A unique blend of modified biochemistry, anatomy and ultrastructure. Biochim Biophys Acta 895: 81-106
9. Matsuoka M (1990) Structure, genetic mapping, and expression of the gene for pyruvate, orthophosphate dikinase from maize. J Biol Chem 265: 16772-16777
10. Pocalyko DJ, Carroll LJ, Martin BM, Babbitt PC and Dunaway-Mariano D (1990) Analysis of sequence homologies in plant and bacterial pyruvate phosphate dikinase, enzyme I of the bacterial phosphoenolpyruvate: sugar phosphotransferase system and other PEP-utilizing enzymes. Identification of potential catalytic and regulatory motifs. Biochemistry 29: 10757-10765
11. Roeske CA and Chollet R (1987) Chemical modification of the bifunctional regulatory protein of maize leaf pyruvate, orthophosphate dikinase: evidence for two distinct active sites. J Biol Chem 262: 12575-12582
12. Roeske CA, Kutny RM, Budde RJA and Chollet R (1988) Sequence of the phosphothreonyl regulatory site peptide from inactive maize leaf pyruvate, orthophosphate dikinase. J Biol Chem 263: 6683-6687
13. Sheen J (1991) Molecular mechanisms underlying the differential expression of maize pyruvate, orthophosphate dikinase genes. Plant Cell 3: 225-245
14. Smith CM, Duff SMG and Chollet R (1994a) Partial purification and characterization of maize-leaf pyruvate,orthophosphate dikinase regulatory protein: A low-abundance, mesophyll-chloroplast stromal protein. Arch Biochem Biophys 308: 200-206
15. Smith CM, Sarath G and Chollet R (1994b) A simple, single-tube radioisotopic assay for the phosphorylation/inactivation activity of the pyruvate,orthophosphate dikinase regulatory protein. Photosynth Res 40: 295-301
16. Usami S, Ohta S, Komari T and Burnell JN (1995) Cold stability of pyruvate, orthophosphate dikinase of Flaveria brownii. Plant Mol Biol 27: 969-980
17. Xu Y, McGuire M, Dunaway-Mariano D and Martin BM (1995a) Separate site catalysis by pyruvate phosphate dikinase as revealed by deletion mutants. Biochemistry 34: 2195-2202
18. 14C] bromopyruvate and site-directed mutagenesis. Biochemistry 34: 2181-2187
19. Yankie L, Xu Y and Dunaway-Mariano D (1995) Location of the catalytic site for phosphoenolpyruvate formation within the primary structure of Clostridium symbiosum pyruvate phosphate dikinase. 2. Site-directed mutagenesis of an essential arginine contained within an apparent P-loop. Biochemistry 34: 2188-2194