Long-term culture of functional hepatocytes on chemically modified collagen gels

Cytotechnology

Volumn 21, Issue 1, 1996, Pages 31-43

  Shimbara, Naoki ^a,d   Atawa, Ryoichi ^a   Takashina, Makoto ^a,d   Tanaka, Keiji ^b   Ichihara, Akira ^c  

^a Biomaterial Research Institute   (Japan)

^b TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

^c TOKUSHIMA BUNRI UNIVERSITY   (Japan)

^d SUMITOMO ELECTRIC INDUSTRIES LTD   (Japan)

Author keywords

chemical modification; collagen gel; hepatocyte; primary culture

Indexed keywords

EID: 0030477435     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00364835     Document Type: Article

References (31)

1. Chomczynski P and Sacchi N (1987) Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162: 156-159.
2. Dunn JCY, Tompkins RG and Yarmusf ML (1992) Hepatocytes in collagen sandwich: Evidence for transcriptional and translational regulation. J. Cell Biol., 116: 1043-1053.
3. Emerman JT, Enami J, Pitelka DR and Nandi S (1977) Hormonal effects on intracellular and secreted casein in cultures of mouse mammary epithelial cells on floating collagen membranes. Proc. Natl. Acid. Sci., 74: 4466-4470.
4. Fujiwara T, Tanaka K, Kuraatori A, Shin S, Yoshimura T, Ichihara A, Tokunaga F, Aruga R, Iwanaga S, Kakizuka A and Nakanishi S (1989) Molecular cloning of cDNA for proteasomes (multicatalytic proteinase complexes) from rat liver: primary structure of the largest component (C2). Biochemistry, 28: 7332-7340.
5. Gallop PM and Seifter S (1963) Preparation and properties of soluble collagens, Mem. Enzymol. 6: 635-641.
6. Gelman RA, Williams BR and Piez KA (1979) Collagen fibril formation. J. Biol. Chem., 254: 180-186.
7. Granner DK and Tomkins GM (1970) Tyrosine aminotransferase. Methods in Enzymology, 17: 664-637.
8. Guguen-Guillouzo C, Clement B, Baffect G, Beaumont C, Morel-Chany E, Glaise D and Guillouzo A (1983) Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type. Exp. Cell Res., 143: 47-54.
9. Guillouzo A and Guguen-Guillouzo C (1986) Isolated and Cultured Hepatocytes. (John Libbey Eurotext, London).
10. Inoue C, Yamamoto H, Nakamura T, Ichihara A and Okamoto H (1989) Nicotinamide prolongs survival of primary cultured hepatocytes without involving loss of hepatocyte-specific functions. J. Biol., Chem., 264: 4747-4750.
11. Isom HC, Secott T, Georgoff I, Woodworm C and Mumman J (1985) Maintenance of differentiated rat hepatocytes in primary culture. Proc. Natl. Acad. Sci. USA, 82: 3252-3256.
12. Kleinman HK, Klebe RJ and Martin GR (1981) Role of collagenous matrices in the adhesion and growth of cells. J. Cell. Biol., 88: 473-485.
13. Koide N, Sakaguchi K, Koide Y, Asano K, Kawaguchi M, Matsushima H, Takenami T, Shinji T, Mori M and Tsuji T (1990) Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent enviorments. Exp. Cell Res., 186: 227-235.
14. Kumatori A, Nakamura T and Ichihara A (1991) Cell-density dependent expression of the c-myc gene in primary cultured rat hepatocytes. Biochem. Biophys. Res. Commun., 178: 480-485.
15. Michalopoulos G and Pitot HC (1975) Primary culture of parenchymal liver cells on collagen membranes: Morphological and biochemical observation. Exp. Cell Res., 94: 70-78.
16. Nakamura T and Ichihata A (1985) Control of growth and expression of differentiated functions of mature hepatocytes in primary culture. Cell Struct. Func., 10: 1-16.
17. Nakamura T, Arakaki R and Ichihara A (1988) Interleukin -1 beta is a potent growth inhibitor of adult rat hepatocytes in primary culture. Exp. Cell Res., 179: 488-497.
18. Nimmni ME and Harkvess RD (1988) Molecular structure and functions of collagen. Collagen (Nimmni, M.E., Eds., CRC Press, Florida), Vol. I: 3-36.
19. Noda C, Ohguri M and Ichihara A ( 1990) developmental and growth-related regulation of expression of serine dehydrogenase mRNA in rat liver. Biochem. Biophys. Res. Commun., 168: 335-342.
20. Reid LM, Abreu SL and Monthomery K (1988) Extracellular matrix and hormonal regulation of synthesis and abundance of messenger RNAs in cultured liver cells. The Liver (Raven Press, New York),: 717-745.
21. + concentration. Proc. Natl. Acad. Sci. USA. 87: 1825-1829.
22. Rojikind M, Gatmaitan Z, Mackensen S, Giambrone MA, Ponce P and Reid LM (1980) Connective tissue biomatrix: its isolation and utilization for long-term cultures of normal rat hepatocytes. J. Cell Biol., 87: 255-263.
23. Sargent TG, Yang M and Bonner J (1981) Nucleotide sequence of cloned rat serum albumin messenger RNA. Proc. Natl. Acad. Sci. USA, 76: 243-246.
24. Sattler CA, Michalopoulos G, Sattler GL and Pitot HC (1978) Ultrastructure of adult rat hepatocytes cultured on floating collagen membranes. Cancer Res. 38: 1539-1549.
25. Schuetz EG, Li D, Omiecinsky CJ, Muller-Eberhard U, Kleinman HK, Elswick B and Guzelian PS (1988) Regulation of gene expression in adult rat hepatocytes cultured on a basement membrane matrix. J. Cell Physiol. 134: 309-323.
26. Shimbara N, Takashina M, Sato C, Iizuka M, Kobayashi S, Tanaka K and Ichihara A (1992) c-Myc expression is down-regulated by cell-cell and cell-extracellular matrix contacts in normal hepatocytes, but not in hepatoma cells. Biochem. Biophys. Res. Commun., 184: 825-831.
27. Tanaka K, Sato M, Tomita Y and Ichihara A (1978) Biochemical studies on liver functions in primary cultured hepatocytes of adult rats. J. Biochem., 84: 937-946.
28. Tanaka S, Avigad G, Eikenberry EF and Brodsky B (1988) Isolation and partial characterization of collagen chains dimerized by sugar-deived cross-links. J. Biol. Chem., 263: 17650-17657.
29. Tomita Y, Abraham S, Noda C and Ichihara A (1994) Pyruvate stimulates hormonal induction of lipogenic enzymes in primary cultured hepatocytes, Biochim. Biophys. Acta, 1170: 253-257.
30. Towbin H, Staehelin J and Gord J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA, 76: 4350-4354.
31. Yuasa C, Tomita Y, Shono M, Ishimura K and Ichihara A (1993) Importance of cell aggregation for expression of liver functions and regeneration demonstrated with primary cultured hepatocytes J. Cell. Physiol., 156: 522-530.