Rapid evaluation of peat-base legume inoculant using immunomagnetic beads for cell retrieval and fluorescent nucleic acid probes for viability analysis

Plant and Soil

Volumn 186, Issue 1, 1996, Pages 75-79

  Olsen, P E ^a   Rice, W A ^a  

^a AGRICULTURE AND AGRI FOOD CANADA   (Canada)

Author keywords

antibody; immunomagnetic; legume inoculant; magnet; Rhizobium

Indexed keywords

RHIZOBIUM;

EID: 0030477356     PISSN: 0032079X     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00035058     Document Type: Conference Paper

References (6)

1. Anonymous 1991 The methods of testing legume inoculant and pre-inoculated seed products. Fertilizers Act, Section 23, Regulations. Feed and Fertilizer Division, Government of Canada, Ottawa. 15 p.
2. Dye M 1994 The enrichment of Rhizobium from a model system using immunomagnetic separation. J. Microbiol. Meth. 19, 235-245.
3. Molloy P, Brydon L, Porter A J and Harris W J 1995 Separation and concentration of bacteria with immobilized antibody fragments. J. Appl. Bacteriol. 78, 359-365.
4. Olsen P E, Rice W A, Bordeleau L M and Biederbeck V O 1994a Analysis and regulation of legume inoculants in Canada: The need for an increase in standards. Plant and Soil 161, 127-134.
5. Olsen P E, Wright S, Collins M and Rice W 1994b Patterns of reactivity between a panel of monoclonal antibodies and forage Rhizobium strains. Appl. Environ. Microbiol. 60, 654-661.
6. Vincent J M 1970 A manual for the practical study of the root-nodule bacteria. International Biological Programme Handbook No. 15. Blackwell Scientific Publications, Ltd., Oxford, UK.