Spodoptero exigua multicapsid nucleopolyhedrovirus deletion mutants generated in cell culture lack virulence in vivo

Journal of General Virology

Volumn 77, Issue 12, 1996, Pages 3127-3134

  Heldens, Jacobus G M ^a   Van Strien, Elisabeth A ^a   Feldmann, Alphons M ^a   Kulcsár, Peter ^a,d   Munoz, Delia ^b   Leisy, Douglas J ^c   Zuidema, Douwe ^a   Goldbach, Rob W ^a   Vlak, Just M ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b PUBLIC UNIVERSITY OF NAVARRE   (Spain)

^c OREGON STATE UNIVERSITY   (United States)

^d INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

[No Author keywords available]

Indexed keywords

INSECTICIDE; POLYHEDRIN; RESTRICTION ENDONUCLEASE; VIRUS DNA;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CONTROLLED STUDY; DELETION MUTANT; DNA LIBRARY; GENE MAPPING; LARVA; LEPIDOPTERA; MOLECULAR CLONING; NONHUMAN; POLYHEDROSIS VIRUS; PRIORITY JOURNAL; RESTRICTION MAPPING; VIRUS CULTURE; VIRUS GENOME; VIRUS MUTANT; VIRUS VIRULENCE;

ANIMALS; CELL LINE; DNA, VIRAL; GENE DELETION; GENOME, VIRAL; NUCLEOPOLYHEDROVIRUS; RESTRICTION MAPPING; SPODOPTERA; VIRULENCE;

ANIMALIA; BETA VULGARIS SUBSP. VULGARIS; CYPOVIRUS; DNA VIRUSES; INSECTA; LEPIDOPTERA; MYTHIMNA UNIPUNCTA; NUCLEOPOLYHEDROVIRUS; SPODOPTERA EXIGUA; SPODOPTERA EXIGUA NUCLEOPOLYHEDROVIRUS; UNIDENTIFIED BACULOVIRUS;

EID: 0030476933     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-77-12-3127     Document Type: Article

References (25)

1. Ayres, M. D., Howard, S. C., Kuzio, J., Lopez-Ferber, M. & Possee, R. D. (1994). The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202, 586-605.
2. Caballero, P., Zuidema, D., Santiago-Alvarez, C. & Vlak, J. M. (1992). Biochemical and biological characterization of four isolates of Spodoptera exigua nuclear polyhedrosis virus. Biocontrol Science and Technology 2, 145-157.
3. Gelernter, W. D. & Federici, B. A. (1986a). Isolation, identification and determination of virulence of a nuclear polyhedrosis virus from the beet army worm, Spodoptera exigua (Lepidoptera: Noctuidae). Environmental Entomology 15, 240-245.
4. Gelernter, W. D. & Federici, B. A. (1986b). Continuous cell line from Spodoptera exigua (Lepidoptera: Noctuidae) that supports the replication of nuclear polyhedrosis viruses from Spodoptera exigua and Autographa californica. Journal of Invertebrate Pathology 48, 199-207.
5. Hara, K., Funakoshi, M., Tsuda, K. & Kawarabata, T. (1993). New Spodoptera exigua cell lines susceptible to Spodoptera exigua nuclear polyhedrosis virus. In Vitro Cellular Developmental Biology 29A, 904-907.
6. Hara, K., Funakoshi, M., Tsuda, K. & Kawarabata, T. (1994). Susceptibility of Lepidopteran cell lines to a Spodoptera exigua (Lepidoptera: Noctuidae) nuclear polyhedrosis virus. Applied Entomology and Zoology 29, 395-402.
7. Hara, K., Funakoshi, M. & Kawarabata, T. (1995). In vivo and in vitro characterization of several isolates of Spodoptera exigua nuclear polyhedrosis virus. Acta Virologica 39, 215-222.
8. Hawtin, R. E., Arnold, K., Ayres, M. D., Zanotto, P. M. A., Howard, S. C., Gooday, G. W., Chappel, L. H., Kitts, P. A., King, L. A. & Possee, R. D. (1995). Identification and preliminary characterization of a chitinase gene in the Autographa californica nuclear polyhedrosis virus genome. Virology 212, 673-685.
9. Hink, F. (1970). Established cell line from the cabbage looper, Trichoplusia ni. Nature 226, 160-175.
10. Kool, M., Voncken, J. W., van Lier, F. L. J., Tramper, J. & Vlak, J. M. (1991). Detection and analysis of Autographa californica nuclear polyhedrosis virus mutants with defective interfering particles. Virology 183, 739-746.
11. Leisy, D. J., Rohrmann, G. F. & Beaudreau, G. S. (1986a). The nucleotide sequence of the polyhedrin gene region from the multiplecapsid baculovirus of Orgyia pseudotsugata. Virology 153, 280-288.
12. Leisy, D. J., Rohrmann, G. F., Nesson, M. & Beaudreau, G. S. (1986b). Nucleotide sequencing and transcriptional analysis of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus p10 gene. Virology 153, 157-167.
13. McCutchen, B. F., Choudray, P. V., Crenshaw, R., Maddox, D. W., Kamtia, S. G., Palekar, N., Vorath, S., Fowler, E., Hammock, B. D. & Maeda, S. (1991). Development of a recombinant baculovirus expressing an insect specific neurotoxin: potential for pest control. Bio/Technology 9, 848-852.
14. Ohkawa, T., Majima, K. & Maeda, S. (1994). A cysteine protease encoded by the baculovirus Bombyx mon nuclear polyhedrosis virus. Journal of Virology 68, 6619-6625.
15. O'Reilly, D. R. & Miller, L. K. (1991). Improvement of a baculovirus pesticide by deletion of the egt gene. Bio/Technology 9, 1086-1089.
16. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Coning: A Laboratory Manual. 2nd edn. New York: Cold Spring Harbor Laboratory.
17. Smits, P. H. & Vlak, J. M. (1994). Registration of the first viral insecticide in the Netherlands: the development of Spod-X, based on Spodoptera exigua nuclear polyhedrosis virus. Mededelingen van de Faculteit Landbouwwwetenschappen van de Rijksuniversiteit Gent 59, 385-392.
18. Smits, P. H., van de Vrie, M. & Vlak, J. M. (1988). Nuclear polyhedrosis virus for control of Spodoptera exigua in glasshouse crops. Entomologia Experimentalis et Applicata 43, 73-80.
19. Stewart, L. M. D., Hirst, M., Lopez-Ferber, M., Merryweather, A. T., Caylay, P. J. & Possee, R. D. (1991). Construction of an improved baculovirus insecticide containing an insect-specific toxin gene. Nature 352, 85-88.
20. Summers, M. D. & Smith, G. E. (1987). A Manual of Methods for Baculovirus and Insect Cell Culture Procedures. Texas Agricultural Experiment Station Bulletin no. 1555.
21. Tomalski, M. D. & L. K. Miller. (1991). Insect paralysis by baculovirus-mediated expression of a mite neurotoxin gene. Nature 325, 85-88.
22. van Strien, E. A., Zuidema, D., Goldbach, R. W. & Vlak, J. M. (1992). Nucleotide sequence and transcriptional analysis of the polyhedrin gene of Spodoptera exigua nuclear polyhedrosis virus. Journal of General Virology 73, 2813-2821.
23. van Strien, E. A., Jansen, B. J. H., Mans, R. M. W., Zuidema, D. & Vlak, J. M. (1996). Sequence and transcriptional analysis of the ubiquitin gene cluster in the genome of Spodoptera exigua nucleopolyhedrovirus. Journal of General Virology 77, 2321-2328.
24. Vlak, J. M. & Smith, G. E. (1982). Orientation of the genome of Autographa californica nuclear polyhedrosis virus: a proposal. Journal of Virology 41, 1118-1121.
25. Zuidema D., van Oers, M. M., van Strien, E. A., Caballero, P. C., Klok, E. J., Goldbach, R. W. & Vlak, J. M. (1993). Nucleotide sequence and transcriptional analysis of the p10 gene of Spodoptera exigua nuclear polyhedrosis virus. Journal of General Virology 74, 1017-1024.