Positive-negative KG cassettes for construction of multi-gene deletions using a single drug marker

Gene

Volumn 183, Issue 1-2, 1996, Pages 153-157

  Ueki, Toshiyuki ^a   Inouye, Sumiko ^a   Inouye, Masayori ^a  

^a RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

Author keywords

Galactokinase; Homologous recombination; Myxococcus xanthus; Protein Ser Thr kinase

Indexed keywords

DNA FRAGMENT; GALACTOKINASE; KANAMYCIN; MARKER;

ANTIBIOTIC RESISTANCE; ARTICLE; CHROMOSOME; DELETION MUTANT; GENE CASSETTE; GENE DELETION; GENE TARGETING; GENETIC RECOMBINATION; MYXOCOCCALES; NONHUMAN; OPERON; PRIORITY JOURNAL; PROMOTER REGION; YEAST;

ANTI-BACTERIAL AGENTS; GALACTOKINASE; GENE DELETION; GENE TARGETING; GENES, BACTERIAL; GENETIC MARKERS; GENETIC VECTORS; KANAMYCIN; KANAMYCIN RESISTANCE; MUTAGENESIS, INSERTIONAL; MYXOCOCCUS XANTHUS; PROTEIN-SERINE-THREONINE KINASES; TRANSFORMATION, BACTERIAL;

BACTERIA (MICROORGANISMS); MYXOCOCCALES; MYXOCOCCUS XANTHUS;

EID: 0030475588     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00546-X     Document Type: Article

References (11)

1. Beck, E., Ludwig, G., Auerswald, E.A., Reiss, B. and Schallar, H. (1982) Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene 19, 327-336.
2. Dworkin, M. and Kaiser, D. (1993) Myxobacteria II. American Society for Microbiology, Washington, DC.
3. Hodgkin, J. and Kaiser, D. (1977) Genetics of gliding motility in Myxococcus xanthus (Myxobacterales): genes controlling movement of single cells. Mol. Gen. Genet. 171, 167-176.
4. Kashefi, A. and Hartzell, P.L. (1995) Genetic suppression and phenotypic masking of a Myxococcus xanthus frzF-defect. Mol. Microbiol. 15, 483-494.
5. McKenney, K., Shimatake, H., Court, D., Schmeissner, U., Brady, C. and Rosenberg, M. (1981) A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. In: Chirikjian, J.S. and Papas, T.S. (Eds.), Gene Amplification and Analysis, II. Elsevier-North-Holland, Amsterdam, pp. 383-415
6. Munoz-Dorado, J., Inouye, S. and Inouye, M. (1991) A gene encoding protein serine/threonine kinase is required for normal development of Myxococcus xanthus, a Gram-negative bacterium. Cell 67, 995-1006.
7. Gay, P., Le Coq, L., Steinmetz, M., Berkelman, T. and Kado, C.I. (1985) Positive selection procedure for entrapment of insertion sequence elements in Gram-negative bacteria. J. Bacteriol. 164, 918-921.
8. Rosenberg, M., Brawner, M., Gorman, U. and Riff, M. (1986) Galactokinase gene fusion in the study of gene regulation in E. coli, streptomyces, yeast, and higher cell systems. In: Setlow, J.K. and Hollaender, A. (Eds.), Genetic Engineering, 8. Plenum Press, New York, pp. 151-179.
9. Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503-517.
10. Udo, H., Munoz-Dorado, J., Inouye, M. and Inouye, S. (1995) Myxococcus xanthus, a Gram negative bacterium, contains a transmembrane protein serine/threonine kinase that blocks the sections of β-lactamase by phosphorylation. Genes Dev. 9, 972-983.
11. Zhang, W., Munoz-Dorado, J., Inouye, M. and Inouye, S. (1992) Identification of a putative eukaryotic-like protein kinase family in the developmental bacterium Myxococcus xanthus. J. Bacteriol. 174, 5450-5453.