Posttranslational modifications of meningococcal pili. Identification of a common trisaccharide substitution on variant pilins of strain C311

Annals of the New York Academy of Sciences

Volumn 797, Issue , 1996, Pages 53-64

  Virji, Mumtaz ^a   Stimson, Elaine ^b   Makepeace, Katherine ^a   Dell, Anne ^b   Morris, Howard R ^b   Payne, Gail ^c   Saunders, Jon R ^c   Moxon, E Richard ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

^c UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESIN; EPIMERASE; GLYCOSYLATED PROTEIN; TRISACCHARIDE;

BACTERIAL VIRULENCE; BACTERIUM ADHERENCE; BACTERIUM MUTANT; BACTERIUM PILUS; CONFERENCE PAPER; NEISSERIA MENINGITIDIS; NONHUMAN; PROTEIN GLYCOSYLATION; PROTEIN PROCESSING;

BACTERIA (MICROORGANISMS); GALE; NEISSERIA MENINGITIDIS;

EID: 0030475125     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.1996.tb52949.x     Document Type: Conference Paper

References (35)

1. VIRJI, M., H. KAYHTY, D. J. P. FERGUSON, C. ALEXANDRESCU, J. E. HECKELS & E. R. MOXON. 1991. The role of pili in the interactions of pathogenic Neisseria with cultured human endothelial cells. Mol. Microbiol. 5: 1831-1841.
2. VIRJI, M., C. ALEXANDRESCU, D. J. P. FERGUSON, J. R. SAUNDERS & E. R. MOXON. 1992. Variations in the expression of pili: The effect on adherence of Neisseria meningitidis to human epithelial and endothelial cells. Mol. Microbiol. 6: 1271-1279.
3. VIRJI, M., J. R. SAUNDERS, G. SIMS, K. MAKEPEACE, D. MASKELL & J. P. FERGUSON. 1993. Pilus-facilitated adherence of Neisseria meningitidis to human epithelial and endothelial cells: Modulation of adherence phenotype occurs concurrently with changes in amino acid sequence and the glycosylation status of pilin. Mol. Microbiol. 10: 1013-1028.
4. DUNN, K. L. R., M. VIRJI & E. R. MOXON. 1995. Investigations into the molecular basis of meningococcal toxicity for human endothelial and epithelial cells: The synergistic effect of LPS and pili. Microb. Pathogen. 18: 81-96.
5. STIMSON, E., M. VIRJI, K. MAKEPEACE, A. DELL, H. R. MORRIS, G. PAYNE, J. R. SAUNDERS, M. P. JENNINGS, S. BARKER, M. PANICO, I. BLENCH & E. R. MOXON. 1995. Meningococcal pilin: A glycoprotein substituted with digalactosyl 2,4-diacetamido-2,4,6-trideoxyhexose. Mol. Microbiol. 17: 1201-1214.
6. HAMADEH, R. M., G. A. JARVIS, M. ESTABROOK & J. McL. GRIFFISS. 1994. Anti-Gal binds to pilus of Neisseria meningitidis: The Iga isotype blocks complement-mediatede killing. In Neisseria 94. J. S. Evans, S. E. Yost, M. C. J. Maiden & I. M. Feavers, Eds.: 132-133. Meriuex UK Ltd.
7. PARGE, H. E., K. T. FOREST, M. J. HICKEY, D. A. CHRISTENSEN, E. D. GETZOFF & J. A. TAINER. 1995. Structure of the fibre-forming protein pilin at a 2.6 A resolution. Nature 378: 32-38.
8. SHARON, N. & R. W. JEANLOZ. 1960. The diaminohexose component of a polysaccharide isolated from Bacillus subtilis. J. Biol. Chem. 235: 1-5.
9. ZEHAVI, U. & N. SHARON. 1973. Structural studies of 4-acetamido-2-amino-2,4,6-trideoxy-D-glucose (N-Acetylbacillosamine), the N-acetyl-diamino sugar of Bacillus licheniformis. J. Biol. Chem. 248: 433-438.
10. TAHARA, Y. & S. G. WILKINSON. 1983. The lipopolysaccharide from Pseudomonas aeruginosa NCTC 8505. Structure of the O-specific polysaccharide. Eur. J. Biochem. 134: 299-304.
11. HERMANSSON, K., P. JANSSON, T. HOLME & B. GUSTAVSSON. 1993. Structural studies of the Vibrio cholerae O:4 O-antigen polysaccharide. Carbohydr. Res. 248: 199-211.
12. WHITTAKER, D. V., L. A. S. PAROLIS & H. PAROLIS. 1994. Escherichia coli K48 capsular polysaccharide: A glycan containing a novel diacetamido sugar. Carbohydr. Res. 256: 289-301.
13. SHASHKOV, A. S., S. CAMPOS-PORTUGUEZ, H. KOCHANOWSKI, A. YOKOTA & H. MAYER. 1995. The structure of the O-specific polysaccharide from Thiobacillus sp. IFO 14570, with three different diaminopyranoses forming the repeating unit. Carbohydr. Res. 269: 157-166.
14. MESSNER, P. & U. B. SLEYTR. 1991. Bacterial surface layer glycoproteins. Glycobiology 1: 545-551.
15. GARDE, T., D. HARRIS, M. VORDERMEIER, R. LATHIGRA, J. IVANYI & D. YOUNG. 1993. Expression of the Mycobacterium tuberculosis 19-kilodalton antigen in Mycobacterium smegmatis: Immunological analysis and evidence of glycosylation. Infect. Immun. 61: 260-267.
16. BISARIA, V. X. & S. MISHRA. 1989. Regulatory aspects of cellulase biosynthesis and secretion. Crit. Revs. Biotech. 9: 61-104.
17. CASTRIC, P. 1995. PilO, a gene required for glycosylation of Pseudomonas aeruginosa 1244 pilin. Microbiology 141: 1247-1254.
18. MOENS, S., K. MICHIELS & J. VANDERLEYDEN. 1995. Glycosylation of the flagellin of the polar flagellum of Azospirillum brasilense, a Gram-negative nitrogen-fixing bacterium. Microbiology 141: 2651-2657.
19. ROBERTSON, J. N., P. VINCENT & M. E. WARD. 1977. The preparation and properties of gonococcal pili. J. Gen. Microbiol. 102: 169-177.
20. GUBISH, E. R., JR., K. C. S. CHEN & T. M. BUCHANAN. 1982. Attachment of gonococcal pili to lectin-resistant clones of Chinese hamster ovary cells. Infect. Immun. 37: 189-194.
21. POTTS, W. J. & J. R. SAUNDERS. 1988. Nucleotide sequence of the structural gene for class I pilin from Neisseria meningitidis: Homologies with the pilE locus of Neisseria gonorrhoeae. Mol. Microbiol. 2: 647-653.
22. ROTHBARD, J. B., R. FERNANDEZ, L. WANG, N. N. H. TENG & G. K. SCHOOLNIK. 1985. Antibodies to peptides corresponding to a conserved sequence of gonococcal pilins block bacterial adhesion. Proc. Natl. Acad. Sci. USA 82: 915-919.
23. NICOLSON, I. J., A. C. F. PERRY, M. VIRJI, J. E. HECKELS & J. R. SAUNDERS. 1987. Localization of antibody-binding sites by sequence analysis of cloned pilin genes from Neisseria gonorrhoeae. J. Gen. Microbiol. 133: 825-833.
24. VIRJI, M., J. S. EVERSON & P. R. LAMBDEN. 1982. Effect of anti-pilus antisera on virulence of variants of Neisseria gonorrhoeae for cultured epithelial-cells. J. Gen. Microbiol. 128: 1095-1100.
25. VIRJI, M. & J. E. HECKELS. 1984. The role of common and type-specific pilus antigenic domains in adhesion and virulence of gonococci for human epithelial-cells. J. Gen. Microbiol. 130: 1089-1095.
26. FUKUDA, M. & O. HINDSGAUL, EDS. 1994. Molecular Glycobiology: Frontiers in Molecular Biology. Oxford University Press.
27. LANGSFORD, M. I., N. R. GILKES, B. SINGH, B. MOSER, R. C. MILLER, R. A. J. WARREN & D. G. KILBURN. 1987. Glycosylation of bacterial cellulases prevents proteolytic cleavage between functional domains. FEBS Lett. 225: 163-167.
28. BERG-FUSSMAN, A., M. E. GRACE, Y. IOANNOU & G. A. GRABOWSKI. 1993. Human acid beta-glucosidase. N-glycosylation site occupancy and the effect of glycosylation on enzymatic activity. J. Biol. Chem. 268: 14861-14866.
29. SMITH, H., J. A. COLE & N. J. PARSONS. 1992. The sialyation of gonococcal lipopolysaccharide by host factors: A major impact on pathogenicity. FEMS Microbiol. Lett. 100: 287-292.
30. JAISON, P. L., V. M. KANNAN, M. GEETHA & P. S. APPUKUTTAN. 1993. Epitopes recognised by serum anti-α-galactoside antibody are present on brain glycoproteins in man. J. Biosci. 18: 187-193.
31. JENNINGS, M. P., P. VAN DER LEY, K. E. WILKS, D. J. MASKELL, J. T. POOLMAN & E. R. MOXON. 1993. Cloning and molecular analysis of the galE gene of Neisseria meningitidis and its role in lipoplysaccharide biosynthesis. Mol. Microbiol. 10: 361-369.
32. VIRJI, M., K. MAKEPEACE, D. J. P. FERGUSON, M. ACHTMAN, J. SARKARI & E. R. MOXON. 1992. Expression of the Opc protein correlates with invasion of epithelial and endothelial cells by Neisseria meningitidis. Mol. Microbiol. 6: 2785-2795.
33. DEVEREUX, J., P. HAEBERLI & O. SMITHIES. 1984. A comprehensive set of sequence-analysis programs for the VAX. Nucl. Acid Res. 12: 387-395.
34. HECKELS, J. E. & M. VIRJI. 1988. Preparation, characterisation and immunochemistry of pili and fimbriae. In Bacterial Cell Surface Techniques. I. C. Hancock & I. Poxton, Eds.: 67-72. John Wiley & Sons Ltd. London.
35. DELL, A. 1990. Preparation and description mass spectrometry of permethyl and peracetyl derivatives of oligosaccharides. Methods Enzymol. 193: 647-660.