Inhibition of RecA-mediated cleavage in covalent dimers of UmuD

Journal of Bacteriology

Volumn 178, Issue 24, 1996, Pages 7304-7307

  Lee, Melissa H ^a   Guzzo, Angelina ^a   Walker, Graham C ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIMER; DISULFIDE; PROTEIN; RECA PROTEIN;

ARTICLE; CONTROLLED STUDY; CROSS LINKING; DISULFIDE BOND; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL;

ESCHERICHIA COLI;

EID: 0030473419     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.24.7304-7307.1996     Document Type: Article

References (28)

1. Baltista, J. R., T. Ohta, T. Nohmi, W. Sun, and G. C. Walker. 1990. Dominant negative umuD mutations decreasing RecA-mediated cleavage suggest roles for intact UmuD in modulation of SOS mutagenesis. Proc. Natl. Acad. Sci. USA 87:7190-7194.
2. Burckhardt, S. E., R. Woodgale, R. H. Scheuermann, and H. Echols. 1988. UmuD mutagenesis protein of Eschericltia coli: overproduction, purification, and cleavage by RecA. Proc. Natl. Acad. Sci. USA 85:1811-1815.
3. Cohen, S., B. J. Knoll, J. W. Little, and D. W. Mount. 1981. Preferential cleavage of phage λ repressor monomers by recA protease. Nature (London) 294:182-184.
4. Eguchi, Y., O. T. Ogawa, and O. H. Ogawa. 1988. Cleavage of bacteriophage Φ80 el repressor by RecA protein. J. Mol. Biol. 202:565-573.
5. Friedberg, E. C., G. C. Walker, and W. Siede. 1995. DNA repair and mutagenesis. ASM Press, Washington, D.C.
6. Gimble, F, S., and R. T. Sauer. 1986. λ repressor inactivation: properties of purified ind proteins in the autodigestion and RccA-mcdiated cleavage reactions. J. Mol. Biol. 192:39-47.
7. Gimble, F. S., and R. T. Sauer. 1989. Lambda repressor mutants that are better substrates for RecA-mediated cleavage. J. Mol. Biol. 206:29-39.
8. Guzzo, A., M. H. Lee, K. Oda, and G. C. Walker. 1996. Analysis of the region between amino acids 30 and 42 of intact UmuD by a monocysteinc approach. J. Bacteriol. 178:7295-7303.
9. Kim, B., and J. W. Little. 1992. Dimerization of a specific DNA-binding protein on the DNA. Science 255:203-206.
10. Kim, B., and J. W. Little. 1993. Lex A and λ cl repressors as enzymes: specific cleavage in an intermolecular reaction. Cell 73:1165-1173.
11. Lee, M. H., T. Ohta, and G. C. Walker. 1994. A monocysteine approach for probing the structure and interactions of the UmuD protein. J. Bacteriol. 176:4825-4837.
12. Lee, M. H., and G. C. Walker. 1996. Interactions of Eschericchia coli UmuD with activated RccA analyzed by cross-linking UmuD monocysteine derivatives. J. Bacteriol. 178:7285-7294.
13. -) mutants of the LexA repressor of Escherichia coli K-12. J. Bacteriol. 170:2163-2173.
14. Lin, L. L., and J. W. Little. 1989. Autodigestion and RecA-dependent cleavage of Ind mutant LexA proteins. J. Mol. Biol. 210:439-452.
15. Little, J. W. 1984. Autodigestion of LexA and phage λ repressors. Proc. Natl. Acad. Sci. USA 81:1375-1379.
16. Little, J. W., S. H. Edmiston, Z. Pacelli, and D. W. Mount. 1980, Cleavage of the Escherichia coli LexA protein by the RecA protease. Proc. Natl. Acad. Sci. USA 77:3225-3229.
17. Nohmi, T., J. R. Battista, L. A. Dodson, and G. C. Walker. 1988. RecA-mediated cleavage activates UmuD for mutagenesis: mechanistic relation-ship between transcriptional derepression and posttranslational activation. Proc. Natl. Acad. Sci. USA 85:1816-1820.
18. Peat, T. S., E. G. Frank, J. P. McDonald, A. S. Levine, R. Woodgate, and W. A. Hendrickson. 1996. Structure of the UmuD' protein and its regulation in response to DNA damage. Nature (London) 380:727-730.
19. Perry, K. L., S. J. Elledge, B. Milchell, L. Marsh, and G. C. Walker. 1985. umuDC and mucAB operons whose products arc required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology. Proc. Natl. Acad. Sci. USA 82:4331-1335.
20. Phizicky, E. M., and J. W. Roberts. 1980. Kinetics of RecA protein-directed inactivation of repressors ofphage lambda and phage P22. J. Mol. Biol. 139: 319-328.
21. Roland, K. L., M. H. Smith, J. A. Rupley, and J. W. Little. 1992. In vitro analysis of mutant LexA proteins with an increased rate of specific cleavage. J. Mol. Biol. 228:395-408.
22. Sassanfar, M., and J. W. Roberts. 1990. Nature of the SOS-inducing signal in Escherichia coli: the involvement of DNA replication. J. Mol. Biol. 212: 79-96.
23. Sauer, R. T., R. R. Yocum, R. F. Doolittle, M. Lewis, and C. O. Pabo. 1982. Homology among DNA-binding proteins suggests use of a conserved supersecondary structure. Nature (London) 298:447-451.
24. Shinagawa, H., H. Iwasaki, T. Kato, and A. Nakata. 1988. RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis. Proc. Natl. Acad. Sci. USA 85:1806-1810.
25. Slilaty, S. N., and J. W. Little. 1987. Lysine-156 and serine-119 are required for LexA repressor cleavage: a possible mechanism. Proc. Natl. Acad. Sci. USA 84:3987-3991.
26. Slilaty, S. N., J. A. Rupley, and J. W. Little. 1986. Intramolecular cleavage of LexA and phage λ repressors: dependence of kinetics on repressor concentration, pH, temperature, and solvent. Biochemistry 25:6866-6875.
27. Woodgate, R., M. Rajagopalan, C. Lu, and H. Echols. 1989. UmuC mutagenesis protein of Escherichia coli: purification and interaction with UmuD and UmuD'. Proc. Natl. Acad. Sci. USA 86:7301-7305.
28. Yu, X., and E. H. Egelman. 1993. The LexA repressor binds within a deep helical groove of the activated RecA filament. J. Mol. Biol. 231:29-40.