Changes in motility patterns during in-vitro culture of fresh and frozen/thawed testicular and epididymal spermatozoa: Implications for planning treatment by intracytoplasmic sperm injection

Human Reproduction

Volumn 11, Issue 11, 1996, Pages 2474-2476

  Edirisinghe, W Rohini ^a   Junk, Stephen M ^a   Matson, Phillip L ^a   Yovich, John L ^a  

^a PIVET Medical Centre   (Australia)

Author keywords

Epididymal spermatozoa; ICSI; In vitro; Motility; Testicular spermatozoa

Indexed keywords

4 (2 HYDROXYETHYL) 1 PIPERAZINEETHANESULFONIC ACID; BUFFER;

ADULT; ARTICLE; AZOOSPERMIA; CASE REPORT; CELL CULTURE; CONTROLLED STUDY; CRYOPRESERVATION; EPIDIDYMIS; HUMAN; HUMAN CELL; HUMAN TISSUE; INTRACYTOPLASMIC SPERM INJECTION; MALE; SPERMATOZOON; SPERMATOZOON MOTILITY; TESTIS; VASECTOMY;

EID: 0030471119     PISSN: 02681161     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.humrep.a019141     Document Type: Article

References (7)

1. Edirisinghe, W.R., Junk, S.M., Matson, P.L. and Yovich, J.L. (1996) Improving the recovery and handling of spermatozoa from testicular homogenates. Hum. Reprod., 11, 1358.
2. Nagy, Z., Liu, J., Cecile, J. et al. (1995) Using ejaculated, fresh, and frozen-thawed epididymal and testicular spermatozoa gives rise to comparable results after intracytoplasmic sperm injection. Fertil. Steril., 63, 808-815.
3. Schoysman, R., Vanderzwalmen, P., Nijs, M. et al. (1994) Pregnancy obtained with human testicular spermatozoa in an in vitro fertilization program. J. Androl., 15 (Suppl.), 10S-13S.
4. Silber, S.J., Van Steirteghem, A.C., Liu, J. et al. (1995) High fertilization and pregnancy rate after intracytoplasmic sperm injection with spermatozoa obtained from testicle biopsy. Hum. Reprod., 10, 148-152.
5. Tucker, M.J., Morton, P.C., Witt, M.A. et al. (1995) Intracytoplasmic injection of testicular and epididymal spermatozoa for treatment of obstructive azoospermia. Hum. Reprod., 10, 486-489.
6. World Health Organization (1992) WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction. 3rd edn. Cambridge University Press, Cambridge, UK.
7. Zhu, J., Tsirigotis, M., Pelekanos, M. et al. (1996) In-vitro maturation of human testicular spermatozoa. Hum. Reprod., 11, 231-232.