Use of13C-13C NOE for the assignment of NMR lines of larger labeled proteins at larger magnetic fields

Journal of the American Chemical Society

Volumn 118, Issue 49, 1996, Pages 12457-12458

  Fischer, Mark W F ^a   Zeng, Lei ^a   Zuiderweg, Erik R P ^a,b  

^a UNIVERSITY OF MICHIGAN   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; NUCLEAR MAGNETIC RESONANCE; NUCLEAR OVERHAUSER EFFECT; PROTEIN STRUCTURE; TECHNIQUE;

EID: 0030470749     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja962200+     Document Type: Article

References (20)

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2. For a review, see: Bax A.; Grzesiek, S. Acc. Chem. Res. 1993, 26, 131.
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4. Bax, A.; Clore, G. M.; Gronenborn, A. M. J. Magn. Reson. 1990, 88, 425.
5. Wang, H.; Zuiderweg, E. R. P. J. Biomol. NMR 1995, 5, 207.
6. cc is 34 Hz.
7. 13C line widths can be dramatically reduced by protein perdeuteration, offering one solution to the transfer efficiency problem in HCCH-COSY. See also: Kushlan, D. M.; LeMaster, D. M. J. Biomol. NMR 1993, 3, 701.
8. Farmer, B. T., II; Venters, R. A. J. Am. Chem. Soc. 1995, 117, 4187.
9. Goldman, M. Quantum Description of High-Resolution NMR in Liquids; Oxford University Press: Oxford, 1988; p 248.
10. See Figure 1A in the following: Zeng, L.; Fischer, M. W. F.; Zuiderweg, E. R. P. J. Biomol. NMR 1996, 7, 157.
11. 13C polarization transfer and, more importantly, loss in recycling efficiency.
12. Cordier, F.; Bratscher, B.; Marion, D. J. Biomol. NMR 1996, 7, 163.
13. The semiconstant time periods (Grzesiek, S.; Bax, A. J. Biomol. NMK 1993, 3, 185.
14. Logan, T. M.; Olejniczak, E. T.; Xu, R. X.; Fesik, S. W. J. Biomol. NMR 1993, 3, 225
15. HCFor more details see the Supporting Information.
16. Solomon, I. Phys. Rev. 1955, 99, 559.
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18. Daragan, V. A. ; Mayo, K. H. Chem. Phys. Lett. 1993, 206, 30.
19. Resonance assignments for the peptide-binding domain of Hsc-70 were obtained in our lab by R. C. Morshauser.
20. Resonance assignments for the peptide-binding domain of DnaK are described: Wang, H. Ph.D. Thesis, The University of Michigan, 1995.