Ethidium bromide fluorescence photo-enhancement in glycerol

European Journal of Histochemistry

Volumn 40, Issue 4, 1996, Pages 323-330

  Galassi, L ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

Concentration quenching; Ethidium bromide; Fluorescence enhancement; Photo bleaching

Indexed keywords

DNA; ETHIDIUM; FLUORESCENT DYE; GLYCEROL;

ANIMAL; ARTICLE; CELL NUCLEUS; ERYTHROCYTE; FLUORESCENCE MICROSCOPY; FROG; METABOLISM; METHODOLOGY;

ANIMALS; CELL NUCLEUS; DNA; ERYTHROCYTES; ETHIDIUM; FLUORESCENT DYES; GLYCEROL; MICROSCOPY, FLUORESCENCE; RANA ESCULENTA;

ANIMALIA; ANURA;

EID: 0030469261     PISSN: 1121760X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. Angerer L.M., and Moudrianakis E.N.: Interaction of ethidium bromide with whole and deproteinized deoxyribonucleo-protein from calf thymus. J. Mol. Biol. 63, 505-521, 1972.
2. Bellin J.S.: Properties of pigments in the bound state: A review. Photochem. Photobiol. 4, 33-44, 1965.
3. Benson D.M., Bryant J., Plant A.L., Gotto A.M., and Smith L.C.: Digital imaging fluorescence microscopy: spatial heterogeneity of photobleaching rate constants in individual cells. J. Cell. Biol. 100, 1309-1323, 1985.
4. Bittman R.: Studies of the binding of ethidium bromide to transfer ribonucleic acid: absorption, fluorescence, ultracentrifugation and kinetic investigations. J. Mol. Biol. 46, 251-268, 1969.
5. 2 and Hoechst 33258 for DNA cytofluorimetry in tumour pathology. Histochemistry 79, 177-191, 1983.
6. Brakenhoff G.J., Visscher K., and Gijsbers E.J.: Fluorescence bleach rate imagins. J. Microsc. 175, 154-161, 1994.
7. Burns V.W.F.: Fluorescence decay time characteristics of the complex between ethidium bromide and nucleic acids. Arch. Biochem. Biophys. 133, 420-424, 1969.
8. Enerbäck L.: Berberine sulphate binding to mast cell polyanions: A cytofluorimetric method for the quantitation of heparin. Histochemistry 42, 301-313, 1974.
9. Golden J.F., and West S.S.: Fluorescence spectroscopic and fading behaviour of Ehrlich's hyperdiploid mouse ascites tumor cell supravitally stained with acridine orange. J. Histochem. Cytochem. 22, 495-505, 1974.
10. Guenza M., and Cuniberti C.: The ethidium bromide dimer. Absorption and fluorescence properties in aqueous solutions. Spectrochim. Acta 44A, 1359-1364, 1988.
11. Hell S., Reiner G., Cremer C., and Stelzer E.H.K.: Aberrations in confocal fluorescence microscopy induced by mismatches in refractive index. J. Microsc. 169, 391-405, 1993.
12. Koppel D.E., CarLson C., and Smilowitz H.: Analysis of heterogeneous fluorescence photobleaching by video kinetics imaging: the method of cumulants. J. Microsc. 155, 199-206, 1989.
13. LePecq J.B., and Paoletti C.: A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterisation. J. Mol. Biol. 27, 87-106, 1967.
14. Longin A., Souchier C., Ffrench M. and Bryon P.A.: Comparison of antifading agents used in fluorescence microscopy: image analysis and laser confocal microscopy study. J. Histochem. Cytochem. 41, 1833-1840, 1993.
15. Olmsted J., Kearns D.R.: Mechanism of ethidium bromide fluorescence enhancement on binding nucleic acids. Biochemistry 16, 3647-3654, 1977.
16. Robeson J.L., and Titon R.D.: Effect of concentration quenching on fluorescence recovery after photobleaching measurements. Biophys. J. 68, 2145-2155, 1995.
17. Rück A., Hildebrandt C., Köllner T., Schneckenburger H., and Steiner R.: Competition between photobleaching and fluorescence increase of photosensitising porphyrins and tetrasulphonated chloroaluminium-phtalocyanine. J. Photochem. Photobiol. 5, 311-319, 1990.
18. Rundquist I., and Enerbäck L.: Millisecond fading and recovery phenomena in fluorescent biological objects. Histochemistry, 47, 79-87, 1976.
19. Sawin K.E., Theriot J.A., and Mitchison T.J.: Photoactivation of fluorescence as a probe for cytoskeletal dynamics in mitosis and cell motility. In Mason W.T. ed.: Fluorescent and luminscent probes for biological activity. New York, Academic Press, 405-419, 1992.
20. Severin E., and Ohnemus B.: UV dose-dependent increase in the Hoechst fluorescence intensity of both normal and BrdU-DNA. Histochemistry 74, 279-291, 1982.
21. Song L., Hennink E.J., Young I.T., and Tanke H.J.: Photobleaching kinetics of fluorescein in quantitative fluorescence microscopy. Biophys. J. 68, 2588-2600, 1995.
22. Soper S.A., Nutter H.L., and Keller R.A.: The photophysical constants of several fluorescent dyes pertaining to ultrasensitive fluorescence spectroscopy. Photochem. Photobiol. 57, 972-977, 1993.
23. Waring M.J.: Complex formation between ethidium bromide and nucleic acids. J. Mol. Biol. 13, 269-282, 1965.
24. West S.S., and Lorinez A.E.: Fluorescence molecular probes in fluorescence microspectrophotometry and microspectropolarimetry. In Thaer A.A. ed.: Quantitative fluorescence techniques in cell biology. Berlin, Springer Verlag, 395-407, 1973.