The site for GTP hydrolysis on the archaeal elongation factor 2 is unmasked by aliphatic alcohols

Biochimie

Volumn 78, Issue 10, 1996, Pages 832-837

  Raimo, G ^a   Masullo, M ^a   Scarano, G ^a   Bocchini, V ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

ADP ribosylation; Archaebacteria; GTP binding protein; GTPpase; Protein synthesis

Indexed keywords

ALKANOL; BARIUM CHLORIDE; ELONGATION FACTOR 2; ETHYLENE GLYCOL; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE;

ARCHAEBACTERIUM; ARTICLE; CONFORMATIONAL TRANSITION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; GRAM NEGATIVE CHEMOLITHOTROPHIC AND METHANE PRODUCING BACTERIA; HYDROLYSIS; HYDROPHOBICITY; NONHUMAN; RIBOSOME;

EID: 0030468601     PISSN: 03009084     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-9084(97)84335-0     Document Type: Article

References (18)

1. 1 Moldave K ( 1985) Eukaryotic protein synthesis. Annu Rev Biochem 54, 1109-1149
2. 2 Kaziro Y (1978) The role of guanosine-5′-triphosphate in polypeptide chain elongation. Biochim Biophys Acta 505, 95-127
3. 3 Parmeggiani A, Sander G (1981) Properties and regulation of the GTPase activities of elongation factor Tu and G, and of initiation factor 2. Mol Cell Biochem 35, 129-158
4. 4 Klink F (1985) Elongation factors. In: The Bacteria (Woese CR, Wolfe R, eds) Academic Press, London, vol 8, 379-410
5. 5 Ævarsson A, Brazhnikov E, Garber M, Zheltonosova J, Chirgadze Yu, Al-Karadaghi S, Svensson LA, Liljas A (1994) Three-dimensional structure of the ribosomal translocase: elongation factor G from Thermus thermophilus. EMBO J 13, 3669-3677
6. 6 Czworkowski J, Wang J, Steitz TA, Moore PB (1994) The crystal structure of elongation factor G complexed with GDP. at 2.7 Å resolution. EMBO J 13, 3661-3668
7. 7 De Vendittis E, Amatruda MR, Masullo M, Bocchini V (1993) Cloning and sequencing of the gene encoding thermostable elongation factor 2 in Sulfolobus solfataricus. Gene 136, 41-48
8. 8 Raimo G, Masullo, M and Bocchini V (1995) Studies on the polypeptide elongation factor 2 from Sulfolobus solfataricus. Interaction with guanosine nucleotides and GTPase activity stimulated by ribosomes. J Biol Chem 270, 21082-21085
9. 9 Masullo M, De Vendittis E, Bocchini V (1994) Archaebacterial elongation factor 1α carries the catalytic site for GTP hydrolysis. J Biol Chem 269, 20376-20379
10. 10 Fasano O, De Vendittis E, Parmeggiani A (1982) Hydrolysis of GTP by elongation factor Tu can be induced by monovalent cations in the absence of other effectors. J Biol Chem 257, 3145-3150
11. 11 De Vendittis E, Masullo M, Bocchini V (1986) The elongation factor G carries the catalytic site for GTP hydrolysis, which is revealed by using 2-propanol in the absence of ribosomes. J Biol Chem 261, 4445-4450
12. 12 Raimo G, Masullo M, Dello Russo A, Parente A, Gambacorta A, Bocchini V (1993) Properties of the purified elongation factor 2 in the thermo-acidophilic archaebacterium Sulfolobus solfataricus. Ital J Biochem 42, 1-11
13. 13 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 261, 680-685
14. 14 Masullo M, Parlato G, De Vendittis E, Bocchini V (1989) Effect of propan-2-ol on enzymic and structural properties of elongation factor G. Biochem J 261, 725-731
15. 15 Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilyzing the principle of protein-dye binding. Anal Biochem 12, 248-254
16. 16 Singer SJ (1962) The properties of proteins in non aqueous solvents. Adv Protein Chem 17, 1-68
17. 17 Raimo G, Masullo M, Parente A, Dello Russo A, Bocchini V (1992) Molecular, functional and structural properties of an archaebacterial elongation factor 2. Biochim Biophys Acta 1132, 127-132
18. 18 Davidova EK, Ovchininnikov LP (1990) ADP-ribosylated elongation factor 2 (ADP-ribosyl-EF-2) is unable to promote tranlocation within the ribosome. FEBS Lett 261, 350-352