Synthesis of a squash-type protease inhibitor by gene engineering and effects of replacements of conserved hydrophobic amino acid residues on its inhibitory activity

Protein Engineering

Volumn 9, Issue 12, 1996, Pages 1241-1246

  Kojima, Shuichi ^a   Miyoshi, Katsunori ^a   Miura, Kin Ichiro ^a  

^a GAKUSHUIN UNIVERSITY   (Japan)

Author keywords

Hydrophobic residues; Inhibitory activity; Protease inhibitor; Protein engineering; Protein folding

Indexed keywords

CUCURBITA MAXIMA; ESCHERICHIA COLI; SUIDAE;

ADENYLATE KINASE; PROTEINASE INHIBITOR; TRYPSIN INHIBITOR;

AMINO ACID SUBSTITUTION; ARTICLE; ESCHERICHIA COLI; GENETIC CONSERVATION; GENETIC ENGINEERING; HYDROPHOBICITY; NONHUMAN; OXIDATION; PRIORITY JOURNAL; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; VEGETABLE;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; ESCHERICHIA COLI; GENE EXPRESSION; MUTATION; PLANT PROTEINS; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; TRYPSIN INHIBITORS;

EID: 0030465344     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/9.12.1241     Document Type: Article

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