L-ascorbic acid regulates growth and metabolism of renal cells: Improvements in cell culture

American Journal of Physiology - Cell Physiology

Volumn 271, Issue 6 40-6, 1996, Pages

  Nowak, Grazyna ^a   Schnellmann, Rick G ^a,b  

^a UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

^b UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

Author keywords

active sodium transport; basal and ouabain sensitive oxygen consumption; gluconeogenesis; glycolysis; lactate consumption; renal proximal tubular cells; sodium coupled glucose transport; sodium potassium adenosinetriphosphatase

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ASCORBIC ACID 2 PHOSPHATE; GAMMA GLUTAMYLTRANSFERASE; GLUCOSE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; LACTIC ACID; SODIUM ION;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DENSITY; CELL GROWTH; CELL METABOLISM; CELL RESPIRATION; CONTROLLED STUDY; ENZYME ACTIVITY; FEMALE; GLUCOSE TRANSPORT; GLYCOLYSIS; KIDNEY CELL; KIDNEY PROXIMAL TUBULE; KIDNEY TUBULE EPITHELIUM; NONHUMAN; OXYGEN CONSUMPTION; PRIORITY JOURNAL; RABBIT;

EID: 0030463882     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.1996.271.6.c2072     Document Type: Article

References (39)

1. Alcain, F. J., and M. I. Buron. Ascorbate on cell growth and differentiation. J. Bioenerg. Biomembr. 26: 393-398, 1994.
2. Aleo, M. D., and R. G. Schnellmann. Regulation of glycolytic metabolism during long-term primary culture of renal proximal tubule cells. Am. J. Physiol. 262 (Renal Fluid Electrolyte Physiol. 31): F77-F85, 1992.
3. Blais, A., F. Jalal, P. Crine, J. Paiement, and A. Berteloot. Increased functional differentiation of rabbit proximal tubule cells cultured in glucose-free media. Am. J. Physiol. 263 (Renal Fluid Electrolyte Physiol. 32): F152-F162, 1992.
4. Bowers-Komro, D. M., and D. B. McCormick. Characterization of ascorbic acid uptake by isolated kidney cells. J. Nutr. 121: 57-64, 1991.
5. Cole, L. A., J. M. Scheid, and R. L. Tannen. Induction of mitochondrial metabolism and pH-modulated ammoniagenesis by rocking LLC-PK1 cells. Am. J. Physiol. 251 (Cell Physiol. 20): C293-C298, 1986.
6. Courjault-Gautier, F., J. Chevalier, C. C. Abboi, D. K. Chopin, and H. J. Toutain. Consecutive use of hormonally defined serum-free media to establish highly differentiated human renal proximal tubule cells in primary culture. J. Am. Soc. Nephrol. 5: 1949-1963, 1995.
7. Dickman, K. G., and L. J. Mandel. Glycolytic and oxidative metabolism in primary renal proximal tubule cultures. Am. J. Physiol. 257 (Cell Physiol. 26): C333-C340, 1989.
8. Dyer, D. K., Y. Kanai, M. A. Heidiger, S. A. Rubin, and H. M. Said. Expression of a rabbit renal ascorbic acid transporter in Xenopus laevis oocytes. Am. J. Physiol. 267 (Cell Physiol. 36): C301-C306, 1994.
9. +-dependent ascorbic acid transport in osteoblast function. Am. J. Physiol. 268 (Cell Physiol. 37): C1430-C1439, 1995.
10. Geesin, J. C., D. Darr, R. Kaufman, S. Murad, and S. R. Pinnell. Ascorbic acid specifically increases type I and type III procollagen mesenger RNA levels in human skin fibroblasts. J. Invest. Dermatol. 90: 420-424, 1988.
11. Goldenberg, H., and E. Schweinzer. Transport of vitamin C in animal and human cells. J. Bioenerg. Biomembr. 26: 359-368, 1994.
12. Goligorsky, M. S., D. Osborne, T. Howard, K. A. Hruska, and I. E. Karl. Hormonal regulation of gluconeogenesis in cultured proximal tubular cells: role of cytosolic calcium. Am. J. Physiol. 253 (Renal Fluid Electrolyte Physiol. 22): F802-F809, 1987.
13. Griner, R. D., M. D. Aleo, and R. G. Schnellmann. The role of short chain fatty acid substrates in aerobic and glycolytic metabolism in primary cultures of renal proximal tubule cells. In Vitro Cell. Dev. Biol. 29A: 649-655, 1993.
14. Gullans, S. R., P. C. Brazy, V. W. Dennis, and L. J. Mandel. Interactions between gluconeogenesis and sodium transport in rabbit proximal tubule. Am. J. Physiol. 246 (Renal Fluid Electrolyte Physiol. 15): F859-F869, 1984.
15. +-dependent adenosine triphosphatase-mediated ion transport in the intact renal cell. J. Biol. Chem. 256: 10319-10328, 1981.
16. Hata, R. I., and H. Senoo. L-Ascorbic acid 2-phosphate stimulates collagen accumulation, cell proliferation, and formation of a three-dimensional tissuelike substance by skin fibroblasts. J. Cell. Physiol. 138: 8-16, 1989.
17. Jung, J. C., S. M. Lee, N. Kadakia, and M. Taub. Growth and function of primary rabbit kidney proximal tubule cells in glucose-free serum-free medium. J. Cell. Physiol. 150: 243-250, 1992.
18. Labarca, C., and K. Paigen. A simple, rapid, and sensitive DNA assay procedure. Anal. Biochem. 102: 344-352, 1980.
19. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275, 1951.
20. Monteil, C., C. Leclere, F. Dantzer, V. Elkaz, J. P. Fillastre, and J. P. Morin. Modulation of glycolysis induction in primary cultures of rabbit kidney proximal tubule cells: the role of shaking, glucose and insulin. Cell Biol. Int. 17: 953-960, 1993.
21. Monteil, C., C. Leclere, J. P. Fillastre, and J. P. Morin. Insulin and glucose impact on functional and morphological differentiation of rabbit proximal tubule cells in primary culture. Renal Physiol. Biochem. 16: 212-221, 1993.
22. Murad, S., S. Tajima, G. R. Johnson, A. Sivarajah, and S. R. Pinnall. Collagen synthesis in cultured human skin fibroblasts: effect of ascorbic acid and its analogs. J. Invest. Dermatol. 81: 158-162, 1983.
23. Nowak, G., and R. G. Schnellmann. Effect of oleic acid on oxidative and glycolytic metabolism in primary cultures of renal proximal tubule cells. Med. Sci. Res. 22: 733-736, 1994.
24. Nowak, G., and R. G. Schnellmann. Integrative effects of EGF on metabolism and proliferation in renal proximal tubular cells. Am. J. Physiol. 269 (Cell Physiol. 38): C1317-C1325, 1995.
25. Nowak, G., and R. G. Schnellmann. Improved culture conditions stimulate gluconeogenesis in primary cultures of renal proximal tubule cells. Am. J. Physiol. 268 (Cell Physiol. 37): C1053-C1061, 1995.
26. Peterkofsky, B. Ascorbate requirement for hydroxylation and secretion of procollagen: relationship to inhibition of collagen synthesis in scurvy. Am. J. Clin. Nutr. 54: 1135S-1140S, 1991.
27. Sahai, A., L. A. Cole, D. L. Clarke, and R. L. Tannen. Rocking promotes differentiated properties in LLC-PK cells by improved oxygenation. Am. J. Physiol. 256 (Cell Physiol. 25): C1064-C1069, 1989.
28. Schnellmann, R. G. Measurement of oxygen consumption. In: Methods in Toxicology, edited by C. A. Tyson and J. M. Frazier. New York: Academic, 1994, p. 128-139.
29. +-adenosine triphosphatase in the mechanism of action of cardiac glycosides. J. Pharmacol. Exp. Ther. 168: 31-41, 1969.
30. Shapiro, I. M., P. S. Leboy, T. Tokuoka, E. Forbes, K. DeBolt, S. L. Adams, and M. Pacifici. Ascorbic acid regulates multiple metabolic activities of cartilage cells. Am. J. Clin. Nutr. 54: 1209S-1213S, 1991.
31. Stevens, J. L., and G. Zhang. Culture of rat kidney proximal tubule epithelial cells in defined medium. In: Methods in Toxicology: In Vitro Biological Systems, edited by C. A. Tyson and J. M. Frazier. New York: Academic, 1993, p. 374-384.
32. Tang, M. J., K. R. Suresh, and R. L. Tannen. Carbohydrate metabolism by primary cultures of rabbit proximal tubules. Am. J. Physiol. 256 (Cell Physiol. 25): C532-C539, 1989.
33. Tang, M. J., and R. L. Tannen. Relationship between proliferation and glucose metabolism in primary cultures of rabbit proximal tubules. Am. J. Physiol. 259 (Cell Physiol. 28): C455-C461, 1990.
34. Thomas, M. E., A. R. Morrison, and G. F. Schreiner. Metabolic effects of fatty acid-bearing albumin on a proximal tubule cell line. Am. J. Physiol. 268 (Renal Fluid Electrolyte Physiol. 37): F1177-F1184, 1995.
35. Toutain, H., and J. P. Morin. Renal proximal tubule cell cultures for studying drug-induced nephrotoxicity and modulation of phenotype expression by medium components. Renal Failure 14: 371-383, 1992.
36. Toutain, H., N. Vauclin-Jacques, J. P. Fillastre, and J. P. Morin. Biochemical, functional, and morphological characterization of a primary culture of rabbit proximal tububle cells. Exp. Cell Res. 194: 9-18, 1991.
37. Wang, Y., and M. Taub. Insulin and other regulatory factors modulate the growth and the phosphoenolpyruvate carboxykinase (PEPCK) activity of primary rabbit kidney proximal tubule cells in serum free medium. J. Cell. Physiol. 147: 374-382, 1991.
38. Wilson, J. X., and S. J. Dixon. Ascorbate concentration in osteoblastic cells is elevated by transforming growth factor-β. Am. J. Physiol. 268 (Endocrinol. Metab. 31): E565-E571, 1995.
39. Wirthensohn, G., and W. G. Guder. Renal substrate metabolism. Physiol. Rev. 66: 469-497, 1986.