Fast Amplification of the Low Density Lipoprotein Receptor Gene and Detection of a Large Deletion by Means of Long Polymerase Chain Reaction

Clinical Chemistry and Laboratory Medicine

Volumn 34, Issue 12, 1996, Pages 955-960

  Bochmann, Heniyk ^a   Gehrisch, Siegmund ^a   Jaross, Werner ^a  

^a UNIVERSITY HOSPITAL CARL GUSTAV CARUS   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

LOW DENSITY LIPOPROTEIN RECEPTOR;

ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; EXON; GENE AMPLIFICATION; GENE DELETION; GENE STRUCTURE; HETEROZYGOTE; HUMAN; HUMAN CELL; INTRON; MOLECULAR GENETICS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION;

EID: 0030463387     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/cclm.1996.34.12.955     Document Type: Article

References (13)

1. Foord OS, Rose EA. Longdistance PCR. PCR Methods and applications 1994; Vol 3, No 6, S149-S161.
2. Cheng S, Chang SY, Gravitt P, Respess R. Long PCR. Nature 1994; 369: 684-685.
3. Bames WM. PCR amplification of up to 35-kb DNA with high fidelity and high yield from X bacteriophage templates. Proc Natl Acad Sci USA 1994; 91: 2216-2220.
4. Boehringer Mannheim. Highyield, highfidelity amplification with the Expand Long Template PCR System. Biochemica 1995; 1: 11-12.
5. Nielson K, Scott B, Bauer JC, Kretz K. (Stratagene Cloning Systems). TaqPlus DNA polymerase for more robust PCR. Strateg Mol Biol 1995; 7: 64-65.
6. Hobbs HH, Brown MS, Russell DW, Goldstein JL. The LDL receptor locus in familial hypercholesterolemia: mutational analysis of a membrane protein. Annu Rev Genet 1990; 24: 133-170.
7. Soutar AK. Familial hypercholesterolemia and LDL receptor mutations. N Engl J Med 1992; 317: 734-737.
8. Ma Y, Betard C, Roy M, Davignon J, Kessling AM,. Identification of a second “French Canadian” LDL receptor gene deletion and development of a rapid method to detect both deletions. Clin Genet 1989; 36: 219-228.
9. Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucl Acids Res 1988; 3: 1215.
10. Südhof TC, Goldstein JL, Brown MS, Russell DW. The LDL receptor gene: a mosaic of exons shared with different proteins. Science 1985; 228: 815-822.
11. Leitersdorf E, Tobin EJ, Davignon J, Hobbs HH. Common lowdensity lipoprotein receptor mutations in the French Canadian population. J Clin Invest 1990; 85: 1014-1023.
12. Rodningen OK, Leren TP. Application of long polymerase chain reaction in the study of the LDL receptor gene. Scand J Clin Lab Invest 1996; 56: 93-96.
13. Cheng S. Longer PCR amplifications. PCR Strategies 1995; 313-324.