Scanning ion analytical microscopy for high-resolution detection of 5-bromo-2′-deoxyuridine incorporation in metaphase chromosomes

Chromosome Research

Volumn 4, Issue 8, 1996, Pages 574-582

  Bourgeois, C A ^a   Dennebouy, R ^b   Gibaud, A ^a   Gerbault Seureau, M ^a   Malfoy, B ^a   Slodzian, G ^b   Galle, P ^c   Dutrillaux, B ^a  

^a CNRS   (France)

^b UNIVERSITÉ PARIS SUD   (France)

^c INSERM   (France)

Author keywords

5 bromo 2 deoxyuridine incorporation; Chromosome structure; Immunostaining; Scanning ion microscopy

Indexed keywords

BROMINE; BROXURIDINE; CARBON; NITROGEN;

ARTICLE; CELL CYCLE S PHASE; CENTROMERE; CHROMOSOME BANDING PATTERN; CHROMOSOME STRUCTURE; CONTROLLED STUDY; DNA SYNTHESIS; GIEMSA STAIN; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE TEST; IN SITU HYBRIDIZATION; METAPHASE CHROMOSOME; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; TECHNIQUE;

BROMINE; BROMODEOXYURIDINE; CELLS, CULTURED; CHROMOSOMES; DNA; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; LYMPHOCYTES; MASS SPECTROMETRY; METAPHASE; MICROSCOPY; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 0030460292     PISSN: 09673849     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02261720     Document Type: Article

References (29)

1. Bick MD, Davidson RL (1974) Total substitution of bromodeoxyuridine for thymidine in the DNA of a bromodeoxyuridine-dependent cell line. Proc Natl Acad Sci USA 71: 2082-2086.
2. Bourgeois CA, Malfoy B, Kokalj-Vokac N, Galle P, Escaig F, Dutrillaux B (1992) Imaging metaphase chromosomes by high-resolution ion microanalysis: preliminary results. Electron Microscopy 3: 165-166.
3. Dolbeare F, Beisker W, Pallavicini MG, Vanderlaan M, Gray JW (1985) Cytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity. Cytometry 6: 521-530.
4. Drouin R, Messier PE, Richer CL (1988) Human chromosome banding specific for electron microscopy. Cytogenet Cell Genet 47: 117-120.
5. Drouin R, Messier PE, Richer CL (1989) DNA denaturation for ultrastructural banding and the mechanism underlying the fluorochrome-photolysis-giemsa technique studied with anti-5-bromodeoxyuridine antibodies. Chromosoma 98: 174-180.
6. Dutrillaux B, Couturier J (1981) La pratique de l'analyse chromosomique. Paris, Masson.
7. Dutrillaux B, Laurent C, Couturier J, Lejeune J (1973) Coloration par l'acridine orange des chromosomes préalablement traités par le 5-bromodeoxyuridine. CR Acad Sci Paris 276: 3179-3181.
8. Fragu P, Brianon C, Fourré C et al. (1992a) SIMS microscopy in the biomedical field. Biol Cell 74: 5-18.
9. Fragu P, Klijanienko J, Gandia D, Halpern S, Armand JP (1992b) Quantitative mapping of 4′-iododeoxyrubicin in metastatic squamous cell carcinoma by secondary ion mass spectrometry (SIMS) microscopy. Cancer Res 52: 974-977.
10. Galle P (1991) Cartographies moléculaires ultrastructurales par microscopie ionique. Médecine/Science 7: 944-952.
11. Gratzner HG (1982) Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science 218: 474-475.
12. Gratzner HG, Leif RC, Ingram DJ, Castro A (1975) The use of antibody specific for bromodeoxyuridine for the immunofluorescent determination of DNA replication in single cells and chromosomes. Exp Cell Res 95: 88-94.
13. Hallegot P, Girod C, Levi-Setti R (1990) Scanning ion microprobe assessment of biological sample preparation techniques. Scan Microsc 4: 605-612.
14. Hindie E, Coulomb B, Galle P (1992) SIMS microscopy: a tool to measure the intracellular concentration of carbon 14-labelled molecules. Biol Cell 74: 89-92.
15. Just W, Autenrieth M, Vogel W (1993) Demonstration of replication patterns on histone-depleted chromosomes. Cytogenet Cell Genet 62: 60-63.
16. Kokalj-Vokac N, Almeida A, Viegas-Péquignot E, Jeanpierre M, Malfoy B, Dutrillaux B (1993) Specific induction of uncoiling and recombination by azacytidine in classical satellite-containing constitutive heterochromatin. Cytogenet Cell Genet 63: 11-15.
17. Latos-Bielenska A, Hameister H, Vogel W (1987) Detection of BrdUrd incorporation in mammalian chromosomes by a BrdUrd antibody. III. Demonstration of replication patterns in highly resolved chromosomes. Hum Genet 76: 293-295.
18. Latt SA (1973) Microfluorimetric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc Natl Acad Sci USA 70: 3395-3399.
19. Lemieux N, Dutrillaux B, Viegas-Péquignot E (1992) A simple method for simultaneous R- or G-banding and fluorescence in situ hybridization of small single-copy genes. Cytogenet Cell Genet 59: 311-312.
20. Levi-Setti R, Le Beau M (1992) Cytogenetic applications of high resolution secondary ion imaging microanalysis: detection and mapping of tracer isotopes in human chromosomes. Biol Cell 74: 51-58.
21. Linton RW, Goldsmith JG (1992) The role of secondary ion mass spectrometry (SIMS) in biological microanalysis: technique comparisons and prospects. Biol Cell 74: 147-160.
22. Perry P, Wolff S (1974) New giemsa method for the differential staining of sister chromatids. Nature 251: 156-158.
23. Raap AK, Marijnen HGJ, Vrolijk J, van der Ploeg M (1986) Denaturation, renaturation and loss of DNA during in situ hybridization procedures. Cytometry 7: 235-242.
24. Richard F, Vogt N, Muleris M, Malfoy B, Dutrillaux B (1994) Increased FISH efficiency using APC probes generated by direct incorporation of labeled nucleotides by PCR. Cytogenet Cell Genet 65: 169-171.
25. Schutte B, Reynders MMJ, Bosman FT, Blijham GH (1987) Studies with anti-bromodeoxyuridine antibodies. II. Simultaneous immunocytochemical detection of antigen expression and DNA synthesis by in vivo labeling of mouse intestinal mucosa. J Histochem Cytochem 35: 371-374.
26. Schweitzer D, Ambros P, Andrle M (1978) Modification of DAPI banding on human chromosomes by prestaining with a DNA-binding oligopeptid antibiotic, distamycin A. Exp Cell Res 111: 327-332.
27. Slodzian G, Daigne B, Girard F, Boust F, Million F (1990) Cartographie parallle de plusieurs éléments ou isotopes par balayage avec une sonde ionique submicronique: premiers résultats. CR Acad Sci Paris 311: 57-64.
28. Viegas-Péquignot E, Dutrillaux B (1978) Une méthode simple pour obtenir des prophases et des prométaphases. Ann Génét 21: 122-125.
29. Vogel W, Schempp W, Fuel V (1978) Silver-staining specificity in metaphases after incorporation of 5-bromodeoxyuridine (BUDR). Hum Génét 40: 199-203.