Expression, purification and characterization of recombinant crambin

Protein Engineering

Volumn 9, Issue 12, 1996, Pages 1233-1239

  Lobb, Leslie ^a   Stec, Boguslaw ^a   Kantrowitz, Evan K ^a   Yamano, Akihito ^a,c   Stojanoff, Vivian ^b   Markman, Ofer ^a   Teeter, Martha M ^a  

^a BOSTON COLLEGE   (United States)

^b BROOKHAVEN NATIONAL LABORATORY   (United States)

^c RIGAKU CORPORATION   (Japan)

Author keywords

Crystal structure of crambin; Expression of hydrophobic protein; Fusion protein; Recombinant crambin

Indexed keywords

ESCHERICHIA COLI;

ALCOHOL; BLOOD CLOTTING FACTOR 10A; BUFFER; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; PHOSPHOLIPID; PROTEINASE; RECOMBINANT PROTEIN; SYNTHETIC DNA; VEGETABLE PROTEIN; WATER;

AFFINITY CHROMATOGRAPHY; AMINO ACID ANALYSIS; ARTICLE; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; CRYSTALLIZATION; ESCHERICHIA COLI; HYDROPHOBICITY; MUTAGENESIS; NONHUMAN; PLANT SEED; PRIORITY JOURNAL; PROTEIN PURIFICATION; SYNCHROTRON;

AMINO ACID SEQUENCE; ATP-BINDING CASSETTE TRANSPORTERS; BASE SEQUENCE; CARRIER PROTEINS; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, SYNTHETIC; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MONOSACCHARIDE TRANSPORT PROTEINS; PLANT PROTEINS; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

EID: 0030460119     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/9.12.1233     Document Type: Article

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