Modification of alpha-amylase functions by protein engineering

Annals of the New York Academy of Sciences

Volumn 799, Issue , 1996, Pages 65-69

  Terashima, Masaaki ^a   Katoh, Shigeo ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMYLASE; STARCH;

BIOTECHNOLOGY; CONFERENCE PAPER; CONTROLLED STUDY; DRUG DEGRADATION; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME KINETICS; ENZYME MODIFICATION; GENETIC ENGINEERING; TEMPERATURE SENSITIVITY; THERMOSTABILITY;

EID: 0030459941     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.1996.tb33179.x     Document Type: Conference Paper

References (9)

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3. TERASHIMA, M., A. KUBO, M. SUZAWA, Y. ITOH & S. KATOH. 1994. The roles of the N-linked carbohydrate chain of rice α-amylase in thermostability and enzyme kinetics. Eur. J. Biochem. 226(1): 249-254.
4. TERASHIMA, M., M. KAWAI, M. H. KUMAGAI, R. L. RODRIGUEZ & S. KATOH. 1995. Characteristics of a chimera enzyme engineered from two rice α-amylase isozymes. Appl. Microbiol. Biotechnol. In press.
5. KUMAGAI, M. H., M. SHAW, M. TERASHIMA, Z. VRKLJAN, J. R. WHITAKER & R. L. RODRIGUEZ. 1990. Expression and secretion of rice α-amylase by Saccharomyces cerevisiae. Gene 94: 209-216.
6. KATOH, S. & M. TERASHIMA. 1994. Purification of secreted α-amylases by immunoaffinity chromatography with cross-reactive antibody. Appl. Microbiol. Biotechnol. 42(1): 36-39.
7. KATOH, S., M. TERASHIMA & M. KOUNO. 1995. Purification of recombinant α-amylase by immunoaffinity chromatography with anti-peptide antibody. Appl. Microbiol. Biotechnol. 43(5): 871-876.
8. KADZIOLA, A., J. ABE, B. SVENSSON & R. HASER. 1994. Crystal and molecular structure of barley α-amylase. J. Mol. Biol. 239: 104-121.
9. BUSH, D. S., L. STICHER, R. HUYSTEE, D. WAGNER & R. L. JONES. 1989. The calcium requirements and enzymatic activity of two isoforms of barley aleurone α-amylase. J. Biol. Chem. 264(32): 19392-19398.