Cloning of cDNA encoding rat spermatidal protein TP2 and expression in Escherichia coli

Protein Expression and Purification

Volumn 8, Issue 4, 1996, Pages 409-415

  Meetei, Amom Ruhikanta ^a   Rao, M R S ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0030457476     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0118     Document Type: Article

References (20)

1. 1. Meistrich, M. L. (1989) Histones and basic nuclear protein transition in mammalian spermatogenesis, in "Histones and Other Basic Nuclear Proteins" (L. S. Hnilica, G. S. Stein, and J. L. Stein, Eds.), p. 165, CRC Press, Boca Raton, FL.
2. 2. Unni, E., and Meistrich, M. L. (1992) Purification and characterization of the rat spermatid nuclear protein TP4. J. Biol. Chem. 267, 25359-25363.
3. 3. Baskaran, R., and Rao, M. R. S. (1990) Interaction of spermatid specific protein TP2 with nucleic acids in vitro. A comparative study with TP1. J. Biol. Chem. 265, 21039-21047.
4. 4. Baskaran, R., and Rao, M. R. S. (1991) Mammalian spermatid specific protein TP2 is a zinc metalloprotein with two finger motifs. Biochem. Biophys. Res. Commun. 179, 1491-1499.
5. 5. Kundu, T. K., and Rao, M. R. S. (1994) Characterization of the zinc-metalloprotein nature of rat spermatidal protein TP2. FEBS Lett. 351, 6-10.
6. 6. Kundu, T. K., and Rao, M. R. S. (1995) DNA condensation by the rat spermatidal protein TP2 shows a GC-rich sequence preference and is zinc dependent. Biochemistry 34, 5143-5150.
7. 7. Luerssen, H., Maier, W. M., Hoyer-Fender, S., and Engel, W. (1988) The nucleotide sequence of rat transition protein 2 (TP2) cDNA. Nucleic Acids Res. 17, 3585.
8. 8. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) "Molecular Cloning: A Laboratory Manual" Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
9. 9. Chomczynski, P., and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium-thioscyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
10. 10. Laemmli, U. K. (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 277, 680-685.
11. 11. Platz, R. D., Meistrich, M. L., and Grimes, S. R., Jr. (1977) Low molecular weight basic proteins in spermatids. Methods Cell Biol. 16, 297-316.
12. 12. Livingstone, D. M. (1974). Immunoaffinity chromatography of proteins. Methods Enzymol. 34, 723-731.
13. 13. Panyim, S., and Chalkley, R. (1969) High resolution acrylamide gel electrophoresis of histones. Arch. Biochem. Biophys. 130, 337-346.
14. 14. Mazen, A., Gradwohl, G., and Mureia, G. D. (1988) Zinc-binding protein detected by protein blotting. Anal. Biochem. 172, 39-42.
15. 15. Kleene, K. C., and Flynn, J. F. (1987) Characterization of a cDNA clone encoding a basic protein TP2 involved in chromatin condensation during spermiogenesis in the mouse. J. Biol. Chem. 262, 17272-17277.
16. 16. Nelson, J. E., and Krawetz, S. A. (1993) Linkage of human spermatid specific basic nuclear protein genes. J. Biol. Chem. 268, 2932-2936.
17. 17. Janknecht, R., de Martynoff, G., Lou, J., Hipskind, R. A., Nordheim, A., and Stunnenberg, H. G. (1991) Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vacciania virus. Proc. Natl. Acad. Sci. USA 88, 8972-8976.
18. 18. Porath, J. (1992) Immobilized metal ion affinity chromatography. Protein Express. Purif. 3, 263-281.
19. 19. Kane, J. F. (1995) Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli. Curr. Op. Biotechnol. 6, 494-500.
20. 20. Barton, G. J. (1993) ALSCRIPT: A tool to format multiple sequence alignments. Protein Eng. 6, 37-40.