Molecular comparison of cultured hybridoma cells that switch isotypes at high and low rates

Somatic Cell and Molecular Genetics

Volumn 22, Issue 4, 1996, Pages 329-340

  Lin, Muquan ^a   Spira, Gad ^b   Scharff, Matthew D ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; B LYMPHOCYTE; CELL DIFFERENTIATION; GENETIC TRANSCRIPTION; HYBRIDOMA; METHYLATION; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; BASE SEQUENCE; DNA METHYLATION; DNA PRIMERS; GENETIC VECTORS; HYBRIDOMAS; IMMUNOGLOBULIN CLASS SWITCHING; IMMUNOGLOBULIN ISOTYPES; KINETICS; MICE; RECOMBINATION, GENETIC; RNA; TRANSCRIPTION, GENETIC; VARIATION (GENETICS);

EID: 0030456271     PISSN: 07407750     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02369570     Document Type: Article

References (56)

1. Radbruch, A., Muller, W., and Rajewsky, K. (1986). Class switch recombination is IgG1 specific on active and inactive IgH loci of IgG1-secreting B-cell blasts. Proc. Natl. Acad. Sci. U.S.A. 83:3954-3957.
2. Kepron, M.R., Chen, Y.W., Uhr, J.W., and Vitetta, E.S. (1989). IL-4 induces the specific rearrangement of gamma 1 genes on the expressed and unexpressed chromosomes of lipopolysaccharide-activated normal murine B cells. J. Immunol. 143:334-339.
3. Esser, C., and Radbruch, A. (1990). Immunoglobulin class switching: molecular and cellular analysis. [Review]. Ann. Rev. Immunol. 8:717-735.
4. Harriman, W., Volk, H., Defranoux, N., and Wabl, M. (1993). Immunoglobulin class switch recombination. [Review]. Ann. Rev. Immunol. 11:361-384.
5. Coffman, R.L., and Carty, J. (1986). A T cell activity that enhances polyclonal IgE production and its inhibition by interferon-gamma. J. Immunol. 136:949-954.
6. Lebman, D.A., and Coffman, R.L. (1988). Interleukin 4 causes isotype switching to IgE in T cell-stimulated clonal B cell cultures. J. Exp. Med. 168:853-862.
7. Snapper, C.M., Finkelman, F.D., and Paul, W.E. (1988). Differential regulation of IgG1 and IgE synthesis by interleukin 4. J. Exp. Med. 167:183-196.
8. Snapper, C.M., and Paul, W.E. (1987). Interferon-gamma and B cell stimulatory factor-1 reciprocally regulate Ig isotype production. Science 236:944-947.
9. Snapper, C.M., McIntyre, T.M., Mandler, R., Pecanha, L.M., Finkelman, F.D., Lees, A., and Mond, J.J. (1992). Induction of IgG3 secretion by interferon gamma: a model for T cell-independent class switching in response to T cell-independent type 2 antigens. J. Exp. Med. 175:1367-1371.
10. Coffman, R.L., Lebman, D.A., and Shrader, B. (1989). Transforming growth factor beta specifically enhances IgA production by lipopolysaccharide-stimulated murine B lymphocytes. J. Exp. Med. 170:1039-1044.
11. McIntyre, T.M., Klinman, D.R., Rothman, P., Lugo, M., Dasch, J.R., Mond, J.J., and Snapper, C.M. (1993). Transforming growth factor beta 1 selectivity stimulates immunoglobulin G2b secretion by lipopolysaccharide-activated murine B cells. J. Exp. Med. 177:1031-1037.
12. Burger, C., and Radbruch, A. (1990). Protective methylation of immunoglobulin and T cell receptor (TcR) gene loci prior to induction of class switch and TcR recombination. Eur. J. Immunol. 20:2285-2291.
13. Schmitz, J., and Radbruch, A. (1989). An interleukin 4-induced DNase I hypersensitive site indicates opening of the gamma 1 switch region prior to switch recombination. Int. Immunol. 1:570-575.
14. Stavnezer-Nordgren, J., and Sirlin, S. (1986). Specificity of immunoglobulin heavy chain switch correlates with activity of germline heavy chain genes prior to switching. EMBO J. 5:95-102.
15. Lutzker, S., and Alt, F.W. (1988). Structure and expression of germ line immunoglobulin gamma 2b transcripts [published erratum appears in Mol Cell Biol 1988 Oct;8(10):4585]. Mol. Cell. Biol. 8:1849-1852.
16. Lutzker, S., Rothman, P., Pollock, R., Coffman, R., and Alt, F.W. (1988). Mitogen- and IL-4-regulated expression of germ-line Ig gamma 2b transcripts: evidence for directed heavy chain class switching. Cell 53:177-184.
17. Stavnezer, J., Radcliffe, G., Lin, Y.C., Nietupski, J., Berggren, L., Sitia, R., and Severinson, E. (1988). Immunoglobulin heavy-chain switching may be directed by prior induction of transcripts from constant-region genes. Proc. Natl. Acad. Sci. U.S.A. 85:7704-7708.
18. Esser, C., and Radbruch, A. (1989). Rapid induction of transcription of unrearranged S gamma 1 switch regions in activated murine B cells by interleukin 4. EMBO J. 8:483-488.
19. Berton, M.T., Uhr, J.W., and Vitetta, E.S. (1989). Synthesis of germ-line gamma 1 immunoglobulin heavy-chain transcripts in resting B cells: induction by interleukin 4 and inhibition by interferon gamma. Proc. Natl. Acad. Sci. U.S.A. 86:2829-2833.
20. Lebman, D.A., Nomura, D.Y., Coffman, R.L., and Lee, F.D. (1990). Molecular characterization of germ-line immunoglobulin A transcripts produced during transforming growth factor type beta-induced isotype switching. Proc. Natl. Acad. Sci. U.S.A. 87:3962-3966.
21. Gauchat, J.F., Lebman, D.A., Coffman, R.L., Gascan, H., and de Vries, J.E. (1990). Structure and expression of germline epsilon transcripts in human B cells induced by interleukin 4 to switch to IgE production. J. Exp. Med. 172:463-473.
22. Jung, S., Rajewsky, K., and Radbruch, A. (1993). Shutdown of class switch recombination by deletion of a switch region control element. Science 259:984-987.
23. Zhang, J., Bottaro, A., Li, S., Stewart, V., and Alt, F.W. (1993). A selective defect in IgG2b switching as a result of targeted mutation of the I-gamma-2b promoter and exon. EMBO J. 12:3529-3537.
24. Harriman, G.R., Bradley, A., Das, S., Rogers-Fani, P., and Davis, A.C. (1996). IgA class switch in I alpha exon-deficient mice. Role of germline transcription in class switch recombination. J. Clin. Invest. 97:477-485.
25. Lorenz, M., Jung, S., and Radbruch, A. (1995). Switch transcripts in immunoglobulin class switching. Science 267:1825-1828.
26. Dunnick, W., Hertz, G.Z., Scappino, L., and Gritzmacher, C. (1993). DNA sequences at immunoglobulin switch region recombination sites [published erratum appears in Nucleic Acids Res 1993 May 11;21(9): 2285]. [Review]. Nucleic Acids Res. 21:365-372.
27. Waters, S.H., Saikh, K.U., and Stavnezer, J. (1989). A B-cell-specific nuclear protein that binds to DNA sites 5′ to immunoglobulin S alpha tandem repeats is regulated during differentiation. Mol. Cell. Biol. 9:5594-5601.
28. Barberis, A., Widenhorn, K., Vitelli, L., and Busslinger, M. (1990). A novel B-cell lineage-specific transcription factor present at early but not late stages of differentiation. Genes Dev. 4:849-859.
29. Williams, M., and Maizels, N. (1991). LR1, a lipopolysaccharide-responsive factor with binding sites in the immunoglobulin switch regions and heavy-chain enhancer. Genes Dev. 5:2353-2361.
30. Wuerffel, R.A., Nathan, A.T., and Kenter, A.L. (1990). Detection of an immunoglobulin switch region-specific DNA-binding protein in mitogen-stimulated mouse splenic B cells. Mol. Cell. Biol. 10:1714-1718.
31. Wuerffel, R., Jamieson, C.E., Morgan, L., Merkulov, G.V., Sen, R., and Kenter, A.L. (1992). Switch recombination breakpoints are strictly correlated with DNA recognition motifs for immunoglobulin S gamma 3 DNA-binding proteins. J. Exp. Med. 176:339-349.
32. Xu, L., Kim, M.G., and Marcu, K.B. (1992). Properties of B cell stage specific and ubiquitous nuclear factors binding to immunoglobulin heavy chain gene switch regions. Int. Immunol. 4:875-887.
33. Liao, F., Giannini, S.L., and Birshtein, B.K. (1992). A nuclear DNA-binding protein expressed during early stages of B cell differentiation interacts with diverse segments within and 3′ of the Ig H chain gene cluster. J. Immunol. 148:2909-2917.
34. Liao, F., Birshtein, B.K., Busslinger, M., and Rothman, P. (1994). The transcription factor BSAP (NF-HB) is essential for immunoglobulin germ-line epsilon transcription. J. Immunol. 152:2904-2911.
35. Spira, G., Aguila, H.L., and Scharff, M.D. (1988). T15 PC-binding monoclonal antibodies retain specificity when they switch from IgM to IgG. J. Immunol. 140:2675-2680.
36. Spira, G., and Scharff, M.D. (1992). Identification of rare immunoglobulin switch variants using the ELISA spot assay. J. Immunol. Methods 148:121-129.
37. Luria, S.E., and Delbruck, M. (1943). Mutations of bacteria from virus sensitivity to virus resistance. Genetics 28:491-511.
38. Lea, D.E., and Coulson, C.A. (1949). The distribution of the numbers of mutants in bacterial populations. J. Genetics 119:264-275.
39. Lin, M.Q., Chang, C.J., and Green, N.S. (1996). Estimation of high rates of mutation and isotype switching in B cells by fluctuation analysis. Mut. Res. 351:105-116.
40. Spira, G., Gregor, P., Aguila, H.L., and Scharff, M.D. (1994). Clonal variants of hybridoma cells that switch isotype at a high frequency. Proc. Natl. Acad. Sci. U.S.A. 91:3423-3427.
41. Marshak-Rothstein, A., Siekevitz, M., Margolies, M.N., Mudgett-Hunter, M. and Gefter, M.L. (1980). Hybridoma proteins expressing the predominant idiotype of the antiazophenylarsonate response of A/J mice. Proc. Natl. Acad. Sci. U.S.A. 77:1120-1124.
42. Greene, G., Hodous, J., Dintzis, R.Z., and Dintzis, H.M. (1990). Modification, optimization and simplification of the spot ELISA technique for the enumeration of cells secreting anti-hapten antibody. J. Immunol. Methods 129:187-197.
43. Zhu, M., Rabinowitz, J.L., Green, N.S., Kobrin, B.J., and Scharff, M.D. (1995). A well-differentiated B-cell line is permissive for somatic mutation of transfected immunoglobulin heavy-chain gene. Proc. Natl. Acad. Sci. U.S.A. 92:2810-2814.
44. Hayashida, H., Miyata, T., Yamawaki-Kataoka, Y., Honjo, T., Weis, J., and Blattner, F. (1984). Concerted evolution of the mouse immunoglobulin gamma chain genes. EMBO J. 3:2047-2053.
45. Pollock, R.R., French, D.L., Gefter, M.L., and Scharff, M.D. (1988). Identification of mutant monoclonal antibodies with increased antigen binding. Proc. Natl. Acad. Sci. U.S.A. 85:2298-2302.
46. French, D.L., Pollock, R.R., Aguila, H.L., and Scharff, M.D. (1991). The molecular and biochemical characterization of mutant monoclonal antibodies with increased antigen binding. J. Immunol. 146:2010-2016.
47. Collins, J.T., and Dunnick, W.A. (1993). Germline transcripts of the murine immunoglobulin gamma-2a gene-structure and induction by IFN-gamma. Int. Immunol. 5:885-891.
48. Casadevall, A., and Scharff, M.D. (1991). The mouse antibody response to infection with Cryplococcus neoformans: VH and VL usage in polysaccharide binding antibodies. J. Exp. med. 174:151-160.
49. Ayares, D., Spencer, J., Schwartz, F., Morse, B., and Kucherlapati, R. (1985). Homologous recombination between autonomously replicating plasmids in mammalian cells. Genetics 111:375-388.
50. Song, K.Y., Chekuri, L., Rauth, S., Ehrlich, S., and Kucherlapati, R. (1985). Effect of double-strand breaks on homologous recombination in mammalian cells and extracts. Mol. Cell. Biol. 5:3331-3336.
51. Hesse, J.E., Lieber, M.R., Gellert, M., and Mizuuchi, K. (1987). Extrachromosomal DNA substrates in pre-B cells undergo inversion or deletion at immunoglobulin V-(D)-J joining signals. Cell 49:775-783.
52. Burger, C., and Radbruch, A. (1992). Demethylation of the constant region genes of immunoglobulins reflects the differentiation state of the B cell. Mol. Immunol. 29:1105-1112.
53. Burrows, P.D., Beck-Engeser, G.B., and Wabl, M.R. (1983). Immunoglobulin heavy-chain class switching in a pre-B cell line is accompanied by DNA rearrangement. Nature 306:243-246.
54. Muller, C.E., and Rajewsky, K. (1983). Isolation of immunoglobulin class switch variants from hybridoma cell lines secreting anti-idiotypic antibodies by sequencial sublining. J. Immunol. 131:877-881.
55. Nikaido, T., Yamawaki-Kataoka Y., and Honjo, T. (1982). Nucleotide sequences of switch regions of immunoglobulin C epsilon and C gamma genes and their comparison. J. Biol. Chem. 257:7322-7329.
56. Kucherlapati, R.S., Eves, E.M., Song, K.Y., Morse, B.S., and Smithies, O. (1984). Homologous recombination between plasmids in mammalian cells can be enhanced by treatment of input DNA. Proc. Natl. Acad. Sci. U.S.A. 81:3153-3157.