Purification and characterization of lactoferrin, lactoperoxidase, lysozyme and immunoglobulins from camel's milk

International Dairy Journal

Volumn 6, Issue 2, 1996, Pages 129-145

  Elagamy, E I ^d   Ruppanner, R ^a   Ismail, A ^d   Champagne, C P ^b   Assaf, R ^c  

^a UNIVERSITÉ LAVAL   (Canada)

^b FOOD RESEARCH AND DEVELOPMENT CENTRE   (Canada)

^c Gouvernement du Quebec   (Canada)

^d NONE

Author keywords

[No Author keywords available]

Indexed keywords

BOVINAE; CAMELIDAE; VISCUM ALBUM;

EID: 0030455418     PISSN: 09586946     EISSN: None     Source Type: Journal    
DOI: 10.1016/0958-6946(94)00055-7     Document Type: Article

References (32)

1. Allen, P. Z. & Morrison, M. (1966). Lactoperoxidase VI. Immunochemical studies on lactoperoxidase from the milk on several species. Arch. Biochem. Biophys., 113, 540-547.
2. Axelsen, N. H., Kroll, J. & Weeke, B. (1973). A Manual of Quantitative Immunoelectrophoresis. Universitetsfolaget, Oslo. pp. 15-35.
3. Barbour, E. K., Nabbut, N. H., Frerichs, W. M. & Al-Nakhli, H. M. (1984). Inhibition of pathogenic bacteria by camel's milk: relation to whey lysozyme and stage of lactation. J. Food prot. 47, 838-840.
4. Butler, J. E. (1971). Physicochemical and immunochemical studies of bovine IgA and glycoprotein-A. Biochim. Biophys. Acta, 251, 435-449.
5. 2b. Immunology Lett., 16, 31-38.
6. 1 dimers in colostrum. Mol. Immunol., 17, 757-768.
7. Collard, A., Pivvon, P., Portetelle, D., Gregoire, R. & Burny, A. (1984). Isolation and purification of bovine immunoglobulins: use of sephacryl S-300 filtration avoids protein precipitation steps. Ann. Vet. Res., 15, 497-501.
8. Duhaiman, A. S. (1988). Purification of camel milk lysozyme and its lytic effect on Escherichia coli and Micrococcus lysodeikticus. Comp. Biochem. Physiol., 91B, 793-796.
9. 2 to protein A Sepharose. Mol. Immunol., 117, 29-36.
10. Ekstrand, B. & Bjorck, L. (1986). Fast protein liquid chromatography of antibacterial components in milk, lactoperoxidase, lactoferrin and lysozyme. J. Chromatogr., 358, 429-433.
11. Elagamy, E. I., Ruppanner, R., Ismail, A., Champagne, C. P. & Assaf, R. (1992). Antibacterial and antiviral activity of camel milk protective proteins. J. Dairy Res. 59, 169-175.
12. 2b immunoglobulins from mouse serum using protein-A sepharose. Immunochemistry, 15, 429-436.
13. Gelman Instruments (1977). Electrophoresis product manual technical bulletin 18, Gelman Sciences Inc., Ann Arbor, MI, U.S.A.
14. Goudswaard, J., Vander Donk, J. A., Van Dam, R. H. & Vaerman, J. P. (1978). Protein A reactivity of various mammalian immunoglobulins. Scand. J. Immunol., 8, 21-28.
15. Gray, G. D., Nickelson, M. M. & Crim, J. A. (1969). The demonstration of two γ-globulin subclasses in the goat. Immunochemistry, 6, 641-644.
16. Groves, M. L. (1971). Milk Proteins, Chemistry and Molecular Biology, vol. 2, H. A. Mckenzie ed., Academic press, New York. pp. 367.
17. Hebert, G. A., Pelham, P. L. & Pittman, B. (1973). Determination of the optimal ammonium sulfate concentration for the fractionation of rabbit, sheep, horse and goat antisera. Appl. Microbiol., 25, 26-36.
18. IDF (1988). Code of practice for preservation of raw milk by lactoperoxidase system. Bulletin N° 234. International Dairy Federation, Brussels, Belgium. 15 pp.
19. Johnstone, A. and Thorpe, R. (1985). Immunochemistry in Practice. Blackwell Scientific Publication Co., London, U.K. pp. 27-30.
20. Kohler, H. & Jenzer, H. (1989). Interaction of lactoperoxidase with hydrogen peroxide: formation of enzyme intermediates and generation of free radicals. Free Radical Biol. Med., 6, 323-339.
21. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature, 227, 680-685.
22. Lie, O., Syed, M. & Solbu, H. (1986). Improved agar plate assays of bovine lysozyme and haemolytic complement activity. Acta Vet. Scand., 27, 23-32.
23. Moldoveanu, Z., Tenovuo, J., Pruitt, K. M., Mansson-Rahemtulla, B. & Mestecky, J. (1983). Antibacterial properties of milk: IgA-peroxidase-lactoferrin interactions. The Secretory Immune system, J. R. McGhee & J. Mestecky eds, Annals of the New York Academy of Sciences, 409, 848-850.
24. Morrison, M. & Hultquist, D. E. (1963). Lactoperoxidase II. Isolation. J. Biol. Chem., 238, 2847-2849.
25. Mukkur, T. K. S. & Froese, A. (1971). Isolation and characterization of IgM from bovine colostral whey. Immunochemistry, 8, 257-264.
26. Reiter, B. (1985). The biological significance and exploitation of the non-immunoglobulins protective proteins in milk: Lysozyme, lactoferrin, lactoperoxidase, xanthineoxidase. Bulletin No. 191/1985 International Dairy Federation, Brussels, Belgium.
27. Shindler, J. S. & Bardsley, W. G. (1975). Steady-state kinetics of lactoperoxidase with ABTS as chromogen. Biochem. Biophys. Res. Commun., 67, 1307-1312.
28. Setcavage, T. M. & Kim, Y. B. (1976). Characterization of porcine serum immunoglobulins IgG, IgM, IgA and the preparation of mono-specific antichain sera. Immunochemistry, 13, 643-652.
29. Vakil, J. R., Chandan, R. C., Parry, R. M. & Shahani, K. M. (1969). Susceptibility of several microorganisms to milk lysozymes. J. Dairy Sci. 52, 1192-1197.
30. Weber, K. & Osborn, M. (1969). The reliability of molecular weight determinations by dodecylsulfate polyacrylamide gel electrophoresis. J. Biol. Chem. 244, 4405-4412.
31. Yoshida, S. (1988). Isolation of lactoperoxidase of 89,000 daltons and a globulin of 81,000 daltons from milk acid whey. J. Dairy Sci. 71, 2021-2027.
32. Yoshida, S. & Ye-Xiuyun (1991). Isolation of lactoperoxidase and lactoferrin from bovine milk rennet whey and acid whey by sulphopropyl cation-exchange chromatography. Neth. Milk and Dairy J. 45, 273-280.