Bacterial expression of an active class Ib chitinase from Castanea sativa cotyledons

Plant Molecular Biology

Volumn 32, Issue 6, 1996, Pages 1171-1176

  Allona, Isabel ^a   Collada, Carmen ^a   Casado, Rosa ^a   Paz Ares, Javier ^b   Aragoncillo, Cipriano ^a  

^a Technical University of Madrid   (Spain)

^b CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

Author keywords

antifungal protein; Castanea sativa; chitinase; seed defence protein

Indexed keywords

CASTANEA SATIVA;

EID: 0030451683     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00041402     Document Type: Article

References (26)

1. Berglund L, Brunstedt J, Nielsen KK, Chen Z, Mikkelsen JD, Marcker KA: A proline-rich chitinase from Beta vulgaris. Plant Mol Biol 27: 211-216 (1995).
2. Bol JF, Linthorst HJ, Cornelissen BJ: Plant pathogenesis-related proteins induced by virus infection. Annu Rev Phytopath 28: 113-138 (1990).
3. Broekaert W, Lee H, Kush A, Chua NH, Raikhel N: Wound-induced accumulation of mRNA containing a hevein sequence in laticifers of rubber tree (Hevea brasiliensis). Proc Natl Acad Sci USA 87: 7633-7637 (1990).
4. Broglie KE, Gaynor JJ, Broglie RM: Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris. Proc Natl Acad Sci USA 83: 6820-6824 (1986).
5. Broglie K, Chet I, Holliday M, Cressman R, Biddle P, Knowlton S, Mauvais CJ, Broglie R: Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Science 254: 1194-1197 (1991).
6. Collada C, Casado R, Fraile A, Aragoncillo C: Basic endochitinases are major proteins in Castanea sativa cotyledons. Plant Physiol 100: 778-783 (1992).
7. Collinge DB, Kragh KM, Mikkelsen JD, Nielsen KK, Rasmussen U, Vad K: Plant chitinases. Plant J 3: 31-40 (1993).
8. Davies JM, Clarke HRG, Bradshaw HD, Gordon MP: Populus chitinase genes: structure, organization, and similarity of translated sequences to herbaceous plant chitinases. Plant Mol Biol 17: 631-639 (1991).
9. Finch-Savage WE, Pramanik SK, Bewley JD: The expression of dehydrin proteins in desiccation-sensitive (recalcitrant) seeds of temperate trees. Planta 193: 478-485 (1994).
10. Higgins DG, Sharp PM: CLUSTAL: a package for performing multiple sequence alignments on a microcomputer. Gene 73: 237-244 (1988).
11. Huang JK, Wen L, Swegle M, Tran HC, Thin TH, Naylor HM, Muthukrishnan S, Reeck GR: Nucleotide sequence of a rice genomic clone that encodes a class I endochitinase. Plant Mol Biol 16: 479-480 (1991).
12. Jach G, Görnhardt B, Mundy J, Logemann J, Pinsdorf E, Leah R, Schell J, Maas C: Enhanced quantitative resistance against fungal disease by combinatorial expression of different barley antifungal proteins in transgenic tobacco. Plant J 8: 97-109 (1995).
13. Leah R, Tommerup H, Svendsen I, Mundy J: Biochemical and molecular characterization of three barley seed proteins with antifungal properties. J Biol Chem 266: 1564-1573 (1991).
14. Lerner DR, Raikhel NV: The gene for stinging nettle lectin (Urtica dioica agglutinin) encodes both a lectin and a chitinase. J Biol Chem 267: 11085-11091 (1992).
15. Liao YC, Kreuzaler F, Reisener HJ, Tiburzy R: Characterization of a wheat class Ib chitinase gene differentially induced in isogenic lines by infection with Puccinia graminis. Plant Sci 103: 177-187 (1994).
16. Melchers LS, Apotheker-de Groot M, van der Knaap JA, Ponstein AS, Sela- Buurlage MB, Bol JF, Cornelissen BJC, van den Elzen PJM, Linthorst HJM: A new class of tobacco chitinases homologous to bacterial exo-chitinases displays antifungal activity. Plant J 5: 469-480 (1994).
17. Neuhaus JM, Sticher L, Meins F, Boller T: A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole. Proc Natl Acad Sci USA 88: 10362-10366 (1991).
18. Payne G, Ahl P, Moyer M, Harper A, Beck J, Meins F, Ryals J: Isolation of complementary DNA clones encoding pathogenesis-related proteins P and Q, two acidic chitinases from tobacco. Proc Natl Acad Sci USA 87: 98-102 (1990).
19. Racusen D: Glycoprotein detection in polyacrylamide gels with thymol and sulfuric acid. Anal Biochem 99: 474-476 (1979).
20. Samac DA, Hironaka CM, Yallaly PE, Shah DM: Isolation and characterization of the genes encoding basic and acidic chitinase in Arabidopsis thaliana. Plant Physiol 93: 907-914 (1990).
21. Schlumbaum A, Mauch F, Vögeli U, Boller T: Plant chitinases are potent inhibitors of fungal growth. Nature 324: 365-367 (1986).
22. Shinshi H, Neuhaus JM, Ryals J, Meins F: Structure of a tobacco endochitinase gene: evidence that different chitinase genes can arise by transposition of sequences encoding a cysteine-rich domain. Plant Mol Biol 14: 357-368 (1990).
23. Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW: Use of T7 RNA polymerase to direct expression of cloned genes. Meth Enzymol 185: 60-89 (1990).
24. Wickner W, Driessen AJM, Hartl FU: The enzymology of protein translocation across the Escherichia coli plasma membrane. Annu Rev Biochem 60: 101-124 (1991).
25. Wirth SJ, Wolf GA: Dye-labelled substrates for the assay and detection of chitinase and lysozyme activity. J Microbiol Meth 12: 197-205 (1990).
26. Zhu Q, Maher EA, Masoud S, Dixon RA, Lamb CJ: Enhanced protection against fungal attack by constitutive co-expression of chitinase and glucanase genes in transgenic tobacco. Bio/technology 12: 807-812 (1994).