TGN38-green fluorescent protein hybrid proteins expressed in stably transfected eukaryotic cells provide a tool for the real-time, in vivo study of membrane traffic pathways and suggest a possible role for ratTGN38

Journal of Cell Science

Volumn 109, Issue 12, 1996, Pages 2915-2926

  Girotti, Milena ^a   Banting, George ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Brefeldin A; Green fluorescent protein; Trans Golgi network

Indexed keywords

BREFELDIN A; CELL MEMBRANE PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; CELL STRUCTURE; GENETIC TRANSFECTION; GOLGI COMPLEX; IMMUNOFLUORESCENCE; INTRACELLULAR TRANSPORT; NONHUMAN; PRIORITY JOURNAL; RAT;

AMINO ACID SEQUENCE; ANIMALS; GLYCOPROTEINS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MEMBRANE GLYCOPROTEINS; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RATS; RECOMBINANT PROTEINS; TEMPERATURE; TRANSFECTION;

ANIMALIA; EUKARYOTA; VICTORIA;

EID: 0030451424     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. Ahle, S., Mann, A., Eichelsbacher, U. and Ungewickel, E. (1988). Structural relationships between clathrin assembly proteins from the Golgi and the plasma-membrane. EMBO J. 7, 919-929.
2. Baron, M. D. and Garoff, H. (1990). Mannosidase II and the 135 k-Da Golgi-specifc antigen recognized by monoclonal antibody 53FC3 are the same dimeric protein. J. Biol. Chem. 265, 19928-19931.
3. Belsham, G. J., Denton, R. M. and Tanner, M. J. (1980). Use of a novel rapid preparation of fat-cell plasma membranes employing Percoll to investigate the effects of insulin and adrenaline on membrane protein phosphorylation within intact fat cells. Biochem. J. 192, 457-467.
4. Brake, B., Braghetta, P., Banting, G., Luzio, J. P. and Stanley, K. K. (1990). A new recombinant DNA strategy for the molecular cloning of rare membrane proteins. Biochem. J. 267, 631-637.
5. Burke, B., Griffiths, G., Reggio, H., Louvard, D. and Warren, G. (1982) A monoclonal antibody against a 135-k Golgi membrane protein. EMBO J. 1, 1621-1628.
6. Chapman, R. E. and Munro, S. (1994). Retrieval of TGN proteins from the cell-surface requires endosomal acidification. EMBO J. 13, 2305-2312.
7. Clague, M. J., Urbe, S., Aniento, F. and Gruenberg, J. (1994). Vacuolar ATPase activity is required for endosomal carrier vesicle formation. J. Biol. Chem. 269, 21-24.
8. Crosby, J. R., Jones, S. M. and Howell, K. E. (1992). TGN38 forms a macromolecular complex with small GTP-binding proteins - functional-studies using a cell-free assay. Mol. Biol. Cell 3, A 308.
9. Cubitt, A. B., Heim, R., Adams, S. R., Boyd, A. E., Gross, L. A. and Tsien, R. Y. (1995). Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20, 448-455.
10. Dittié, A. S., Hajibagheri, N. and Tooze, S. A. (1996). The AP-1 adaptor complex binds to immature secretory granules from PC12 cells, and is regulated by ADP-ribosylation factor. J. Cell Biol. 132, 523-536.
11. Heim, R., Prasher, D. C. and Tsien, R. Y. (1994). Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Nat. Acad. Sci. USA 91, 12501-12504.
12. Horn, M. and Banting, G. (1994). Okadaic acid treatment leads to a fragmentation of the trans-Golgi network and an increase in expression of TGN38 at the cell-surface. Biochem. J. 301, 69-73.
13. Kaether, C. and Gerdes, H. H. (1995). Visualization of protein-transport along the secretory pathway using green fluorescent protein. FEBS Lett. 369, 267-271.
14. Ladinsky, M. S. and Howell, K. E. (1992). The trans-Golgi network can be dissected structurally and functionally from the cisternae of the Golgi-complex by brefeldin-A. Eur. J. Cell Biol. 59, 92-105.
15. Lippincott-Schwartz, J., Yuan, L., Tipper, C., Amherdt, M., Orci, L. and Klausner, R. D. (1991). Brefeldin-As effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic. Cell 67, 601-616.
16. Luzio, J. P. and Banting, G. (1993). Eukaryotic membrane traffic - retrieval and retention mechanisms to achieve organelle residence. Trends Biochem. Sci. 18, 395-398.
17. Matlin, K. S. and Simons, K. (1983). Reduced temperature prevents transfer of a membrane glycoprotein to the cell-surface but does not prevent terminal glycosylation Cell 34, 233-243.
18. Pelham, H. R. B. and Munro, S. (1993). Sorting of membrane-proteins in the secretory pathway. Cell 75, 603-605.
19. Ponnambalam, S., Girotti, M., Yaspo, M.-L., Owen, C. W., Perry, A. C. F., Suganuma, T., et al. (1996). Primate homologues of rat TGN38: primary structure, expression and functional implications. J. Cell Sci. 109, 675-685.
20. Rabouille, C., Hui, N., Hunte, F., Kieckbusch, R., Berger, E. G., Warren, G., et al. (1995). Mapping the distribution of Golgi enzymes involved in the construction of complex oligosaccharides. J. Cell Sci. 108, 1617-1627.
21. Reaves, B. and Banting, G. (1992). Perturbation of the morphology of the trans-Golgi network following brefeldin-A treatment - redistribution of a TGN-specific integral membrane-protein. TGN38. J. Cell Biol. 116, 85-94.
22. Reaves, B., Horn, M. and Banting, G. (1993). TGN38/41 recycles between the cell-surface and the TGN - brefeldin-A affects its rate of return to the TGN. Mol. Biol. Cell 4, 93-105.
23. Reaves, B. and Banting, G. (1994a). Overexpression of TGN38/41 leads to mislocalisation of gamma-adaptin. FEBS Lett. 351, 448-456.
24. Reaves, B. and Banting, G. (1994b). Vacuolar ATPase inactivation blocks recycling to the trans-Golgi network from the plasma-membrane. FEBS Lett. 345, 61-66.
25. Robinson, M. S. and Kreis, T. E. (1992). Recruitment of coat proteins onto Golgi membranes in intact and permeabilized cells - effects of Brefeldin-A and G-protein activators. Cell 69, 129-138.
26. Saraste, J. and Kuismanen, E. (1984). Pre-Golgi and post-Golgi vesicles operate in the transport of semliki forest virus membrane-glvcoproteins to the cell-surface. Cell 38, 535-549.