Medium and biocatalyst engineering as a means to affect enzyme activity and stability in organic media

Annals of the New York Academy of Sciences

Volumn 799, Issue , 1996, Pages 318-323

  Öste Triantafyllou, Angeliki ^a   Wehtje, Ernst ^a   Adlercreutz, Patrick ^a   Mattiasson, Bo ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CHYMOTRYPSIN; ORGANIC SOLVENT; SORBITOL;

BIOENGINEERING; CATALYSIS; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME MODIFICATION; ENZYME STABILITY; GENETIC ENGINEERING; TEMPERATURE SENSITIVITY;

EID: 0030451118     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.1996.tb33219.x     Document Type: Conference Paper

References (6)

1. DORDICK, J. S. 1988. Biocatalysis in nonaqueous media. Appl. Biochem. Biotechnol. 19: 103-112.
2. RUPLEY, J. A., E. GRATTON & G. CARERI. 1983. Water and globular proteins. Trends Biochem. Sci. 8: 18-22.
3. ÖSTE-TRIANTAFYLLOU, A., P. ADLERCREUTZ & B. MATTIASSON. 1993. Influence of the reaction medium on enzyme activity in bio-organic synthesis: behavior of lipase from Candida rugosa in the presence of polar additives. Biotechnol. Appl. Biochem. 17: 167-179.
4. ÖSTE-TRIANTAFYLLOU, A., E. WEHTJE, P. ADLERCREUTZ & B. MATTIASSON. 1995. Effects of sorbitol addition on the action of free and immobilized hydrolytic enzymes in organic media. Biotechnol. Bioeng. 45: 406-414.
5. CHOWDHRY, B. Z. & S. C. COLE. 1989. Differential scanning calorimetry: applications in biotechnology. Trends Biotechnol. 7: 11-18.
6. ÖSTE-TRIANTAFYLLOU, A., E. WEHTJE, P. ADLERCREUTZ & B. MATTIASSON. 1995. Calorimetric studies on α-chymotrypsin preparations in air and in organic media. Biochim. Biophys. Acta. In press.