Characterization of tryptophan synthase alpha subunit mutants of Arabidopsis thaliana

Molecular and General Genetics

Volumn 253, Issue 3, 1996, Pages 353-361

  Radwanski, E R ^a,b   Barczak, A J ^a   Last, R L ^a  

^a United States Department of Agriculture Agricultural Research Service and Boyce Thompson Institute for Plant Research   (United States)

^b Department of Obstetrics Gynecology   (United States)

Author keywords

Amino acid biosynthesis; Auxotroph; Plant; TRP3; trpA; TSA1

Indexed keywords

ANTHRANILIC ACID METHYL ESTER; INDOLE DERIVATIVE; TRYPTOPHAN DERIVATIVE; TRYPTOPHAN SYNTHASE;

ALPHA CHAIN; ARABIDOPSIS; ARTICLE; CHROMOSOME 3; DNA SEQUENCE; ELECTROPHORETIC MOBILITY; ENZYME SUBUNIT; GENETIC COMPLEMENTATION; MUTANT; NONHUMAN; PLANT; PRIORITY JOURNAL; STRUCTURAL GENE;

ARABIDOPSIS; CHROMOSOME MAPPING; MUTATION; PHENOTYPE; PLANT PROTEINS; TRYPTOPHAN SYNTHASE;

EID: 0030451030     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380050331     Document Type: Article

References (39)

1. Barczak AJ, Zhao J, Pruitt KD, Last RL (1995) 5-Fluoroindole resistance identifies tryptophan synthase beta subunit mutants in Arabidopsis thaliana. Genetics 140: 303-313
2. Bell CJ, Ecker JR (1994) Assignment of 30 microsatellite loci to the linkage map of Arabidopsis. Genome 19: 137-144
3. Bender J, Fink GR (1995) Epigenetic control of an endogenous gene family is revealed by a novel blue fluorescent mutant of Arabidopsis. Cell 83: 725-734
4. Berlyn MB, Last RL, Fink GR (1989) A gene encoding the tryptophan synthase β subunit of Arabidopsis thaliana. Proc Natl Acad Sci USA 86: 4604-4608
5. Burns DM, Yanofsky C (1989) Nucleotide sequence of the Neurospora crassa trp-3 gene encoding tryptophan synthetase and comparison of the trp-3 polypeptide with its homologs in Saccharomyces cerevisiae and Escherichia coli. J Biol Chem 264: 3840-3848
6. Delmer DP, Mills SE (1968) Tryptophan synthase from Nicotiana tabacum. Biochim Biophys Acta 167: 431-443
7. Demmig-Adams B, Adams WW III (1992) Photoprotection and other responses of plants to high light stress. Annu Rev Plant Physiol Plant Mol Biol 43: 559-626
8. r 76 000 each. Eur J Biochem 102: 159-165
9. Fankhauser H, Pythoud F, King PJ (1990) A tryptophan auxotroph of Hyoscyamus muticus lacking tryptophan-synthase activity. Planta 180: 297-302
10. Hagen KW, Ruiz-Echevarria MJ, Quan Y, Peltz SW (1995) Characterization of cis-acting sequences and decay intermediates involved in nonsense-mediated mRNA turnover. Mol Cell Biol 15: 809-823
11. Haughn GW, Somerville C (1986) Sulfonylurea resistant mutants of Arabidopsis thaliana. Mol Gen Genet 204: 430-434
12. Haughn GW, Smith J, Mazur B, Somerville C (1988) Transformation with a mutant Arabidopsis acetolactate synthase gene renders tobacco resistant to sulfonylurea herbicides. Mol Gen Genet 211: 266-271
13. Klee HJ, Yanofsky MF, Nester EW (1985) Vectors for transformation of higher plants. Bio/Technology 3: 637-642
14. Konieczny A, Ausubel FM (1993) A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J 4: 403-410
15. Last RL (1993) The genetics of nitrogen assimilation and amino acid biosynthesis in flowering plants: progress and prospects. Int Rev Cytol 143: 297-330
16. Last RL, Fink GR (1988) Tryptophan-requiring mutants of the plant Arabidopsis thaliana. Science 240: 305-310
17. Last RL, Bissinger PH, Mahoney DJ, Radwanski ER, Fink GR (1991) Tryptophan mutants in Arabidopsis: the consequences of duplicated tryptophan synthase β genes. Plant Cell 3: 345-358
18. Li J, Last RL (1996) The Arabidopsis thaliana trp5 mutant has a feedback-resistant anthranilate synthase and elevated soluble tryptophan. Plant Physiol 110: 51-59
19. Li J, Chen S, Zhu L, Last RL (1995a) Isolation of cDNAs encoding the tryptophan pathway enzyme indole-3-glycerol phosphate synthase from Arabidopsis thaliana. Plant Physiol 108: 877-878
20. Li J, Zhao J, Rose AB, Schmidt R, Last RL (1995b) Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic analysis of triplicate tryptophan pathway genes. Plant Cell 7: 447-461
21. Lim W, Sarkar SK, Hardman JK (1991a) Enzymatic properties of mutant Escherichia coli tryptophan synthase α-subunits. J Biol Chem 266: 20205-20212
22. Lim W, Sarkar SK, Hardman JK (1991b) Relative activities and stabilities of mutant Escherichia coli tryptophan synthase α subunits. J Bacteriol 173: 1886-1893
23. Lister C, Dean C (1993) Recombinant inbred lines for mapping RFLP and phenotypic markers in Arabidopsis thaliana. Plant J 4: 745-750
24. Matchett WH, DeMoss JA (1975) The subunit structure of tryptophan synthase from Neurospora crassa. J Biol Chem 250: 2941-2946
25. Miles EW (1991) Structural basis for catalysis by tryptophan synthase. Adv Enzymol 64: 93-172
26. Miles EW (1994) Tryptophan synthase: structure, function and protein engineering. In: Biswas BB, Roy S (eds) Proteins: Structure, Function and Engineering. Subcellular Biochemistry Series, vol 24. Plenum Presss, New York, pp 207-254
27. Nagao JT, Moore TC (1972) Partial purification and properties of tryptophan synthase of pea plants. Arch Biochem Biophys 149: 402-413
28. Niyogi KK, Last RL, Fink GR, Keith B (1993) Suppressors of trp1 fluorescence identify a new Arabidopsis gene, TRP4, encoding the anthranilate synthase beta subunit. Plant Cell 5: 1011-1027
29. Radwanski ER (1995) Arabidopsis thaliana tryptophan synthase alpha: isolation, analysis of expression, and mutant characterization. PhD Dissertation, Cornell University, Ithaca, New York
30. Radwanski ER, Last RL (1995) Tryptophan biosynthesis and metabolism: biochemical and molecular genetics. Plant Cell 7: 921-934
31. Radwanski ER, Zhao J, Last RL (1995) Arabidopsis thaliana tryptophan synthase alpha: gene cloning, expression, and subunit interaction. Mol Gen Genet 248: 657-667
32. Rose AB, Casselman AL, Last RL (1992) A phosphoribosylanthranilate transferase gene is defective in blue fluorescent Arabidopsis thaliana tryptophan mutants. Plant Physiol 10: 582-592
33. Sibold L, Henriquet M (1988) Cloning of the trp genes from the archaebacterium Methanococcus voltae: nucleotide sequence of the trpBA genes. Mol Gen Genet 214: 430-450
34. Smith OH, Yanofsky C (1962) Enzymes involved in the biosynthesis of tryptophan. Meth Enzymol 5: 794-806
35. Takano E, Maki M, Mori H, Hatanaka M, Marti T, Titani K, Kannagi R, Ooi T, Murachi T (1988) Pig heart calpastatin: identification of repetitive domain structures and anomalous behavior in polyacrylamide gel electrophoresis. Biochemistry 27: 1964-1972
36. Wright AD, Moehlenkamp CA, Perrot GH, Neuffer MG, Cone KC (1992) The maize auxotrophic mutant orange pericarp is defective in duplicate genes for tryptophan synthase β. Plant Cell 4: 711-719
37. Yanofsky C, Crawford IP (1987) The tryptophan operon. In: FC Neidhardt, JL Ingraham, KB Low, B Magasanik, M Schaechter, HE Umbarger (eds), Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology, Washington DC, pp 1453-1472
38. Zalkin H, Yanofsky C (1982) Yeast gene TRP5: structure, function, and regulation. J Biol Chem 257: 1491-1500
39. Zhao J, Last RL (1995) Immunological characterization and chloroplast import of the tryptophan biosynthetic enzymes of the flowering plant Arabidopsis thaliana. J Biol Chem 270: 6081-6087