Binding and kinetic properties of HIV-1 reverse transcriptase markedly differ during initiation and elongation of reverse transcription

EMBO Journal

Volumn 15, Issue 24, 1996, Pages 7178-7187

  Lanchy, Jean Marc ^a   Ehresmann, Chantal ^a   Le Grice, Stuart F J ^b   Ehresmann, Bernard ^a   Marquet, Roland ^a  

^a INSTITUT DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

HIV 1; Kinetics; Polymerase; Replication; Retrovirus

Indexed keywords

LYSINE TRANSFER RNA; OLIGODEOXYRIBONUCLEOTIDE; RNA DIRECTED DNA POLYMERASE; SIGMA FACTOR;

ANIMAL TISSUE; ARTICLE; CHEMICAL MODIFICATION; DISSOCIATION CONSTANT; ENZYME BINDING; ENZYME KINETICS; ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION; TRANSCRIPTION INITIATION;

EID: 0030449991     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.1460-2075.1996.tb01109.x     Document Type: Article

References (37)

1. Trp primer is required for efficient initiation of reverse transcription. J. Virol., 66, 2464-2472.
2. Aiyar,A., Ge,Z. and Leis,J. (1994) A specific orientation of RNA secondary structure is required for initiation of reverse transcription. J. Virol., 68, 611-618.
3. Arnold,E. et al. (1992) Structure of HIV-1 reverse transcriptase DNA complex at 7-Å resolution showing active site locations. Nature, 357, 85-89.
4. Baltimore,D. (1970) Viral RNA-dependent DNA polymerase. Nature, 226, 1209-1211.
5. Beard,W.A. and Wilson,S.H. (1993) Kinetic analysis of template-primer interactions with recombinant forms of HIV-1 reverse transcriptase. Biochemistry, 32, 9745-9753.
6. DeStefano,J.J., Bambara,R.A. and Fay,P.J. (1993) Parameters that influence the binding of human immunodeficiency virus reverse transcriptase to nucleic acid structures. Biochemistry, 32, 6908-6915.
7. Met and genomic RNA of the yeast Ty1 retrotransposon. Nucleic Acids Res., 24, 441-449.
8. Gangloff,J., Pouyet,J., Kern,D. and Dirheimer,G. (1984) A quenched-flow apparatus which allows the measurement of the kinetics of a reaction in one stroke. J. Biochem. Biophys. Methods, 9, 201-213.
9. Gilboa,E., Mitra,S.W., Goff,S. and Baltimore,D. (1979) A detailed model of reverse transcription and tests of crucial aspects. Cell, 18, 93-100.
10. Hsieh,J.C., Zinnen,S. and Modrich,P. (1993) Kinetic mechanism of the DNA-dependent DNA polymerase activity of human immunodeficiency virus reverse transcriptase. J. Biol. Chem., 268, 24607-24613.
11. Lys modulate primer/template loop-loop interaction in the initiation complex of HIV-1 reverse transcription. J. Biol. Chem. 268, 25269-25272.
12. Lys (template/primer) complex. J. Mol. Biol., 247, 236-250.
13. Lys. EMBO J., 15, 917-924.
14. Jacobo-Molina,A. et al. (1993) Crystal structure of human immunodeficiency virus type-1 reverse transcriptase complexed with double-stranded DNA at 3.0 angstrom resolution shows bent DNA. Proc. Natl Acad. Sci. USA, 90, 6320-6324.
15. Jaju,M., Beard,W.A. and Wilson,S.H. (1995) Human immunodeficiency virus type 1 reverse transcriptase - 3′-azidodeoxythymidine 5′-triphosphate inhibition indicates two-step binding for template-primer. J. Biol. Chem., 270, 9740-9747.
16. Kati,W.M., Johnson,K.A., Jerva,L.F. and Anderson,K.S. (1992) Mechanism and fidelity of HIV reverse transcriptase. J. Biol. Chem., 267, 25988-25997.
17. Kohlstaedt,L.A., Wang,J., Friedman,J.M., Rice,P.A. and Steitz,T.A. (1992) Crystal structure at 3,5 Å resolution of HIV-1 reverse transcriptase complexed with an inhibitor. Science, 256, 1783-1790.
18. Le Grice,S.F.J. and Grueninger-Leitch,F. (1990) Rapid purification of homodimer and heterodimer HIV-1 reverse transcriptase by metal chelate affinity chromatography. Eur. J. Biochem., 187, 307-314.
19. Marquet,R., Baudin,F., Gabus,C., Darlix,J.L., Mougel,M., Ehresmann,C. and Ehresmann,B. (1991) Dimerization of human immunodeficiency virus (type 1) RNA: stimulation by cations and possible mechanism. Nucleic Acids Res., 19, 2349-2357.
20. Marquet,R., Isel,C., Ehresmann,C. and Ehresmann,B. (1995) tRNAs as primer of reverse transcriptase. Biochimie, 77, 113-124.
21. Martin,C.T., Muller,D.K. and Coleman,J.E. (1988) Processivity in early stages of transcription by T7 RNA polymerase. Biochemistry, 27, 3966-3974.
22. McClure,W.R. (1985) Mechanism and control of transcription initiation in prokaryotes. Annu. Rev. Biochem., 54, 171-204.
23. Patel,H.P., Jacobo-Molina,A., Ding,J., Tantillo,C., Clark,A.D.J., Raag,R., Nanni,R.G., Hughes,S.H. and Arnold,E. (1995) Insights into DNA polymerization mechanisms from structure and function analysis of HIV-1 reverse transcriptase. Biochemistry, 34, 5351-5363.
24. Patel,S.S., Wong,I. and Johnson,K.A. (1991) Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. Biochemistry, 30, 511-525.
25. Reardon,J.E. (1992) Human immunodeficiency virus reverse transcriptase - steady-state and pre-steady-state kinetics of nucleotide incorporation. Biochemistry, 31, 4473-4479.
26. Reardon,J.E. (1993) Human immunodeficiency virus reverse transcriptase - a kinetic analysis of RNA-dependent and DNA-dependent DNA polymerization. J. Biol. Chem., 268, 8743-8751.
27. Rittinger,K., Divita,G. and Goody,R.S. (1995) Human immunodeficiency virus reverse transcriptase substrate-induced conformational changes and the mechanism of inhibition by nonnucleoside inhibitors. Proc. Natl Acad Sci. USA, 92, 8046-8049.
28. Shimamoto,T., Hsu,M.Y., Inouye,S. and Inouye,M. (1993) Reverse transcriptases from bacterial retrons require specific secondary structures at the 5′-end of the template for the cDNA priming reaction. J. Biol. Chem., 268, 2684-2692.
29. Spence,R.A., Kati,W.M., Anderson,K.S. and Johnson,K.A. (1995) Mechanism of inhibition of HIV-1 reverse transcriptase by nonnucleoside inhibitors. Science, 267, 988-993.
30. Temin,H.M. and Mizutani,S. (1970) RNA-dependent DNA polymerase in virions of rous sarcoma virus. Nature, 226, 1211-1213.
31. von Hippel,P.H., Bear,D.G., Morgan,W.D. and McSwiggen,J.A. (1984) Protein-nucleic acid interactions in transcription: a molecular analysis. Annu. Rev. Biochem., 53, 389-446.
32. His. J. Virol., 70, 966-975.
33. Wang,H. and Lambowitz,A.M. (1993) The mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor. Cell, 75, 1071-1081.
34. Wang,G.H. and Seeger,C. (1992) The reverse transcriptase of hepatitis-B virus acts as a protein primer for viral DNA synthesis. Cell, 71, 663-670.
35. Wilhelm,M., Wilhelm,F.X., Keith,G., Agoutin,B. and Heyman,T. (1994) Yeast ty1 retrotransposon: the minus-strand primer binding site and a cis-acting domain of the ty1 RNA are both important for packaging of primer tRNA inside virus-like particles. Nucleic Acids Res., 22, 4560-4565.
36. Wong,I., Patel,S.S. and Johnson,K.A. (1981) An induced-fit kinetic mechanism for DNA replication fidelity: direct measurement by single-turnover kinetics. Biochemistry, 30, 526-537.
37. Zimmerly,S., Guo,H.T., Perlman,P.S. and Lambowitz,A.M. (1995) Group II intron mobility occurs by target DNA-primed reverse transcription. Cell, 82, 545-554.