Alterations of yeast artificial chromosome transgenic sequences in stretched embryonic stem-cell chromatin visualized by fluorescence in situ hybridization

Cytogenetics and Cell Genetics

Volumn 75, Issue 1, 1996, Pages 67-70

  Rosenberg, C ^a   Voltz, A K ^a   Lamb, B T ^a   Stetten, G ^a   Gearhart, J D ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CHROMATIN; DNA TRANSFECTION; FLUORESCENCE IN SITU HYBRIDIZATION; GENE INSERTION SEQUENCE; GENE NUMBER; HUMAN; HUMAN CELL; MOLECULAR CLONING; MOUSE; NONHUMAN; PRIORITY JOURNAL; STEM CELL; TRANSGENE; TRANSGENIC MOUSE; YEAST ARTIFICIAL CHROMOSOME;

ANIMALS; CELL LINE; CHROMATIN; CHROMOSOMES, ARTIFICIAL, YEAST; HUMANS; HYBRID CELLS; IN SITU HYBRIDIZATION, FLUORESCENCE; MICE; MICE, TRANSGENIC; RESTRICTION MAPPING; STEM CELLS; SUPEROXIDE DISMUTASE; TRANSFECTION; TRANSGENES;

EID: 0030449830     PISSN: 03010171     EISSN: 1424859X     Source Type: Journal    
DOI: 10.1159/000134459     Document Type: Article

References (14)

1. Brownstein BW, Silverman GA, Lillie RD, Burke DT, Korsmeyer SJ, Schlessingcr D, Olson MV: Isolation of single copy genes from a library of yeast artificial chromosome cloneS, Science 264:1348-1351 (1989).
2. Damverse JG, Wiegant J, Raap AK, Breuning MH, van Ommen GJ: Multiple colors by fluorescence in situ hybridization using ratio-labelled DNA probes create a molecular karyotypE, Hum molec Genet 1:593-598(1992).
3. Elroy-Stein O. Bemstein Y, Groner Y: Overproduction of human Cu/Zn- superoxide dismulase in transfected cells: extenuation of paraquat-mediated cy-toxicitv and enhancement of lipid peroxidatioN, EMBOJ 5:615-622(1986).
4. Fidlerova H, Senger G, Kost M, Sanseau P, Sheer D: Two simple procedures for releasing chromatin from routinely fixed cells for fluorescence in situ hybridizatioN, Cytogenet Cell Genet 65:203-205 (1994).
5. Florijn RJ, Blonden LAJ, Vrolijk H, Wiegant J, Vaan-drager JW. Baas F, den Dunnen JT, Tanke HJ, van Ommen GJ, Raap AK: High-resolution DNA Fi-bcr-FISH for genomic DNA mapping and colour bar-coding of large geneS, Hum molec Genet 4:831-836(1995).
6. Heiskancn M, Kaarhu R, Helisten E, Peltonen L, Kal-lioniemi OP, Palotie A: High resolution mapping using fluorescence in situ hybridization to extended DNA fibers prepared from agarose-embed-ded cellS, Biotechniques 17:928-933 (1994).
7. Hong HH, Tsui LC: Free chromatin mapping by FISH, Meth molec Biol 33:109-122 (1994).
8. Lamb BT, Sisodia SS, Lawler AM, Slunt HH, Kearns WG, Pearson PL, Price DL, Gearhart JD: Introduction and expression of the 400 kilobase amyloid precursor protein gene in transgenic micE, Nature Genet 5:22-30(1993).
9. Parra I, Windle B: High resolution visual mapping of stretched DNA by fluorescent hybridizatioN, Nature Genet 5:17-21 (1993).
10. Rosenberg C, Florijn RJ, Van de Rijkc FM, Bionden LA, Raap AK, van Ommen GJ, den Dunnen JT: High-resolution DNA Fiber-FISH on yeast artificial chromosomes: direct visualization of DNA replicatioN, Nature Genet 10:477-479(1995).
11. Schriick E, du Manoir S, Veldman B, Shoell B, Wien-berg J, Fcrguson-Smith MA, Ning Y, Ledbetter DH, Bar-Am I, Soenksen D, Garini Y, Ried T: Multicolor spectral karyotyping of human chromosomeS, Science 273:494-497 (1996).
12. Speicher MR, Ballard SG, Ward DC: Karyotyping human chromosomes by combinatorial multi-fluor FISH, Nature Genet 12:368-375 (1996).
13. Voltz AK: The generation and analysis of embryonic stem cells and micc that express the human copper zinc superoxide dismutase gene cloned within a yeast artificial chromosomE, PhD Thesis, The Johns Hopkins University, Baltimore MD(1995).
14. Wiegant J, Kalle W. Mullenders L. Brookes S, Hoovers JM, Dauwerse JG, van Ommen GJ, Raap AK: High-resolution in situ hybridization using DNA halo preparationS, Hum molec Genet 1:587-591 (1992).