Ammonia-excreting mutants of Klebsiella pneumoniae with a pleiotropic defect in nitrogen metabolism

Archives of Microbiology

Volumn 166, Issue 6, 1996, Pages 388-393

  Kuczius, Thorsten ^a,b   Kleiner, Diethelm ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

^b HUMBOLDT UNIVERSITY   (Germany)

Author keywords

amino acid utilization; ammonia excretion; ammonium metabolism; glnAntrBC; nitrogen control; nitrogenase regulation; Ntr

Indexed keywords

AMMONIA;

ARTICLE; GENETIC ANALYSIS; KLEBSIELLA PNEUMONIAE; MUTANT; NITROGEN METABOLISM; PHENOTYPE; PRIORITY JOURNAL;

AMINO ACIDS; AMMONIA; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE LIBRARY; GLUTAMATE-AMMONIA LIGASE; GLUTAMINE; KLEBSIELLA PNEUMONIAE; MUTAGENESIS; NITROGEN; NITROGENASE; PLASMIDS; TRANSAMINASES;

ENTEROBACTERIACEAE; KLEBSIELLA PNEUMONIAE;

EID: 0030446729     PISSN: 03028933     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002030050400     Document Type: Article

References (39)

1. Allikmets R, Gerrard B, Court D, Dean M (1993) Cloning and organization of the abc and mdl genes of Escherichia coli: relationship to eukaryotic multidrug resistance. Gene 136:231-236
2. Bergmeyer U (1974) Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim
3. Calvo JM, Matthews RG (1994) The leucine-responsive regulatory protein, a global regulator of metabolism in Escherichia coli. Microbiol Rev 58:466-490
4. Castano I, Bastarrachea F, Covarrubias A (1988) gltBDF Operon of Escherichia coli. J Bacteriol 170:821-827
5. -). Arch Microbiol 139:245-247
6. Espin G, Alvarez-Morales A, Merrick M (1981) Complementation analysis of glnA-linked mutations which affect nitrogen fixation in Klebsiella pneumoniae. Mol Gen Genet 184:213-217
7. Espin G, Alvarez-Morales A, Cannon F, Dixon R, Merrick M (1982) Cloning of the glnA, ntrB and ntrC genes of Klebsiella pneumoniae and studies of their role in regulation of the nitrogen fixation (nif) gene cluster. Mol Gen Genet 186:518-524
8. Fawcett JK, Scott JE (1960) A rapid and precise method for the determination of urea. J Clin Pathol 13:156-159
9. Goa J (1953) A microbioret method for protein determination. Scand J Clin Lab Invest 5:218-222
10. Groisman EA, Castilho BA, Casadaban MJ (1984) In vitro DNA cloning and adjacent gene fusion with a mini-Mu-lac bacteriophage containing a plasmid replicon. Proc Natl Acad Sci USA 81:1480-1483
11. Holmes DS, Quigley M (1981) A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114:193-197
12. Holtel A, Merrick M (1988) Identification of the Klebsiella pneumoniae glnB gene: nucleotide sequence of the wild type and mutant alleles. Mol Gen Genet 215:134-138
13. Kleiner D (1985) Bacterial ammonium transport. FEMS Microbiol Rev 32:87-100
14. Kleiner D (1991) Regulation des Stickstoff-Katabolismus bei Bakterien. Bioforum 14:118-122
15. Kleiner D, Paul W, Merrick M (1988) Construction of multicopy expression vectors for regulated overproduction of proteins in Klebsiella pneumoniae and other enteric bacteria. J Gen Microbiol 134:1779-1784
16. Kuczius T, Eitinger T, D Ari R, Castorph H, Kleiner D (1991) The gltF gene of Klebsiella pneumoniae: cloning and initial characterization. Mol Gen Genet 229:479-482
17. Kyhse-Andersen J (1984) Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biochem Biophys Methods 10:203-209
18. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277:680-685
19. Magasanik B (1988) Reversible phosphorylation of an enhancer binding protein regulates the transcription of bacterial nitrogen utilization genes. Trends Biochem Sci 13:475-479
20. Magasanik B (1993) The regulation of nitrogen utilization in enteric bacteria. J Cell Biochem 51:34-40
21. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
22. Masterson RV, Prakash RK, Atherly AG (1985) Conservation of symbiotic nitrogen fixation gene sequences in Rhizobium japonicum and Bradyrhizobium japonicum. J Bacteriol 163:21-26
23. Merrick MJ, Edwards R (1995) Nitrogen control in bacteria. Microbiol Rev 59:604-622
24. Merrick M, Hill S, Hennecke H, Hahn M, Dixon R, Kennedy C (1982) Repressor properties of the nifL gene product in Klebsiella pneumoniae. Mol Gen Genet 185:75-81
25. Merrick M, Gibbins JR, Postgate JR (1987) A rapid and efficient method for plasmid transformation of Klebsiella pneumoniae and Escherichia coli. J Gen Microbiol 133:2053-2057
26. Miller J (1972) Experiments in molecular genetics. Cold Spring Habor Laboratory, Cold Spring Harbor, NY
27. + transporter from plants. EMBO J 13:3464-3471
28. Pengra RM, Wilson PW (1958) Physiology of nitrogen fixation by Aerobacter aerogenes. J Bacteriol 75:21-25
29. Rej R, Horder M (1983) Aspartate aminotransferase (glutamate oxaloacetate transaminase). In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol 3. Verlag Chemie, Weinheim, pp 416-423
30. Rieder G, Merrick MJ, Castorph H, Kleiner D (1994) Function of hisF and hisH gene products in histidine biosynthesis. J Biol Chem 269:14386-14390
31. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467
32. Shanmugam KT, Morandi C (1976) Amino acids as repressors of nitrogenase biosynthesis in Klebsiella pneumoniae. Biochim Biophys Acta 437:322-332
33. Siewe RM, Weil B, Burkovski A, Eikmanns BJ, Eikmanns M, Krmer R (1996) Functional and genetic characterization of the (methyl)ammonium uptake carrier of Corynebacterium glutamicum. J Biol Chem 271:5398-5403
34. Takeshita S, Sato M, Toba M, Masahashi W, Hashimoto-Gotoh T (1987) High-copy-number and low-copy-number plasmid vectors for lacZ-complementation and chloramphenicol- or kanamycin-resistance selection. Gene 61:63-74
35. Tyler B (1978) Regulation of the assimilation of nitrogen compounds. Annu Rev Biochem 47:1127-1162
36. 11 in Escherichia coli: a new regulatory protein in the glutamine synthetase cascade. FEMS Microbiol Lett 132:153-157
37. 2 fixing bacteria - the special case of Rhizobium. FEMS Microbiol Lett 15:61-63
38. 3-dependent NAD synthetase. J Bacteriol 176:3400-3402
39. 11 protein. J Bacteriol 176:108-114