Purification and properties of a starch granule-degrading α-amylase from potato tubers

Journal of Experimental Botany

Volumn 47, Issue 304, 1996, Pages 1789-1795

  Witt, Wolfgang ^a   Sauter, Jõrg J ^a  

^a UNIVERSITY OF KIEL   (Germany)

Author keywords

enzyme purification; potato tuber; Solanum; starch degradation; Amylase

Indexed keywords

SOLANUM; SOLANUM TUBEROSUM; TUBEROSUM;

EID: 0030445615     PISSN: 00220957     EISSN: None     Source Type: Journal    
DOI: 10.1093/jxb/47.11.1789     Document Type: Article

References (34)

1. Bailey KM, Phillips IDJ, Pitt D. 1978. The roles of buds and gibberellin in dormancy and the mobilization of reserve materials in potato tubers. Annals of Botany 41, 649-57.
2. Beck E, Ziegler P. 1989. Biosynthesis and degradation of starch in higher plants. Annual Review of Plant Physiology and Plant Molecular Biology 40, 95-117.
3. Beers EP, Duke SH. 1990. Characterization of α-amylase from shoots and cotyledons of pea (Pisum sativum L.) seedlings. Plant Physiology 92, 1154-63.
4. Bertoft E, Andtfolk C, Kulp S-E. 1989. Effect of pH, temperature, and calcium ions on barley malt α-amylase isoenzymes. Journal of the Institute of Brewing 90, 298-302.
5. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Analytical Biochemistry 72, 248-54.
6. Bulpin PV, ap Rees T. 1978. Starch breakdown in the spadix of Arum maculatum. Phytochemistry 17, 391-6.
7. Cottrell JE, Duffus CM, Paterson L, Mackay GR, Allison MJ, Bain H. 1993. The effect of storage temperature on reducing sugar concentration and the activities of three amylolytic enzymes in tubers of the cultivated potato, Solanum tuberosum L. Potato Research 36, 107-17.
8. Cottrell JE, Duffus CM, Paterson L, Mackay GR. 1995. Properties of potato starch: effects of genotype and growing conditions. Phytochemistry 40, 1057-64.
9. Davies HV, Ross HA. 1987. Hydrolytic and phosphorolytic enzyme activity and reserve mobilization in sprouting tubers of potato (Solanum tuberosum L.).Journal of Plant Physiology 126, 387-96.
10. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28, 350-6.
11. Hirasawa E, Yamamoto S. 1991. Properties and synthesis de novo of auxin-induced α-amylase in pea cotyledons. Planta 184, 438-42.
12. Jacobsen JV, Hanson AD, Chandler PC. 1986. Water stress enhances expression of an α-amylase gene in barley leaves. Plant Physiology 80, 350-9.
13. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277, 680-5.
14. MacGregor A. 1993. Relationships between structure and activity in the α-amylase family of starch-metabolising enzymes. Starch 45, 232-7.
15. MacGregor A, Svensson B. 1989. A super-secondary structure predicted to be common to several α-1,4-D-glucan-cleaving enzymes. Biochemical Journal 259, 145-52.
16. MacGregor AW, Ballance DL. 1980. Hydrolysis of large and small starch granules from normal and waxy barley cultivars by alpha-amylases from barley malt. Cereal Chemistry 57, 397-402.
17. MacGregor AW, Morgan JE. 1986. Hydrolysis of barley starch granules by alpha-amylases from barley malt. Cereal Foods World 31, 688-93.
18. Morrell S, ap Rees T. 1986. Control of the hexose content of-potato tubers. Phytochemistry 25, 1073-6.
19. Nowak J. 1977. Biochemical changes in stored potato tubers with different rest periods. II. Influence of the storage temperature and isopropyl phenylcarbamates on enzyme activities. Journal of Plant Physiology 81, 125-40.
20. Okita TW, Greenberg E, Kuhn DN, Preiss J. 1979. Subcellular localization of the starch degradative and biosynthetic enzymes of spinach leaves. Plant Physiology 64, 187-92.
21. Sauve MD, Ho DT, Roberge M. 1995. Concentration of dilute protein for gel electrophoresis. Analytical Biochemistry 226, 382-3.
22. Sowokinos J. 1990. Stress-induced alterations in carbohydrate metabolism. In: Vayda ME, Park WD, eds. The molecular and cellular biology of the potato. Wallingford, UK: CAB International, 137-58.
23. Steup M. 1988. Starch degradation. In: Preiss J, ed. Biochemistry of plants, Vol. 14. San Diego: Academic Press, 255-96.
24. Sun Z, Henson CA. 1990. Degradation of native starch granules by barley α-glucosidases. Plant Physiology 94, 320-7.
25. Svensson B. 1994. Protein engineering in the α-amylase family: catalytic mechanism, substrate specificity, and stability. Plant Molecular Biology 25, 141-57.
26. Wegrzyn T, MacRae E. 1995. Alpha-amylase and starch degradation in kiwifruit. Journal of Plant Physiology 147, 19-28.
27. Werdan K, Heldt HW, Milovancev M. 1975. The role of the pH in the regulation of carbon fixation in chloroplast stroma. Biochimica et Biophysica Acta 396, 276-92.
28. Witt W, Buchholz A, Sauter JJ. 1995. Binding of endoamylase to native starch grains from poplar wood. Journal of Experimental Botany 46, 1761-9.
29. Witt W, Sauter JJ. 1994a. Enzymes of starch metabolism in poplar wood during fall and winter. Journal of Plant Physiology 143, 625-31.
30. Witt W, Sauter JJ. 1994b. Starch metabolism in poplar ray cells during spring mobilization and summer deposition. Physiologia Plantarum 92, 9-16.
31. Witt W, Sauter JJ. 1995a. Partial purification of amylases and starch phosphorylases from poplar wood. Journal of Plant Physiology 146, 15-21.
32. Witt W, Sauter JJ. 1995b. In vitro degradation of starch grains by phosphorylases and amylases from poplar wood. Journal of Plant Physiology 146, 35-40.
33. Witt W, Sauter JJ. 1996. Purification and characterization of α-amylase from poplar leaves. Phytochemistry 41, 365-72.
34. Ziegler P. 1988. Partial purification and characterization of the major endoamylase of mature pea leaves. Plant Physiology 86, 659-66.