ESI contrast analysis: A new approach for element analysis with energy-filtering transmission electron microscopy (EFTEM)

Journal of Microscopy

Volumn 184, Issue 3, 1996, Pages 175-184

  Kortje K H ^a  

^a UNIVERSITY OF HOHENHEIM   (Germany)

Author keywords

calcium; EELS; EFTEM; ESI; image analysis; image EELS; localization; microanalysis; phosphorus

Indexed keywords

CALCIUM; ELECTRON ENERGY LEVELS; ELECTRON ENERGY LOSS SPECTROSCOPY; ELECTRON PROBE MICROANALYSIS; ELECTRON SCATTERING; ELECTRONS; ENERGY DISSIPATION; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; IMAGE ANALYSIS; MAPPING;

ELECTRON ENERGY-LOSS SPECTROSCOPIES; ELECTRON SPECTROSCOPIC IMAGING; ELEMENTAL MAPPING; ENERGY-FILTERING TRANSMISSION ELECTRON MICROSCOPIES; IMAGE-ANALYSIS; IMAGE-ELECTRON ENERGY-LOSS SPECTROSCOPY; IMAGING CONTRAST; LOCALISATION; NEW APPROACHES;

PHOSPHORUS;

CALCIUM; PHOSPHORUS;

ARTICLE; ELECTRON MICROSCOPY; MICROANALYSIS; PRIORITY JOURNAL;

EID: 0030445586     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1996.1290688.x     Document Type: Article

References (18)

1. Ahn, C.C. & Krivanek, O.L. (1983) EELS Atlas. A Reference Guide of Electron Energy Loss Spectra Covering All Stable Elements. Gatan Inc., Warrendale and HREM Facility.
2. Borgers, M., Thoné, F. & van Nueten, J.M. (1981) The subcellular distribution of calcium and the effects of calcium-antagonists as evaluated with a combined oxalate-pyroantlmonate technique. Acta Histochem. Suppl. XXIV, 327-332.
3. Colliex, C. & Mory, C. (1994) Scanning transmission electron microscopy of biological structures. Biol. Cell, 80, 175-180.
4. De Bruijn, W.C., Sorber, C.W.J., Gelsema, E.S., Beckers, A.L.D. & Jongkind, J.F. (1993) Energy-filtering transmission electron microscopy of biological specimens. Scanning Microsc. 7, 693-709.
5. Egerton, R.F. (1986) Electron Energy-Loss Spectroscopy in the Electron Microscope. Plenum Press, New York.
6. Jourlin, M., Pinoli, J.-C. & Zeboudj, R. (1989) Contrast definition and contour detection for logarithmic images. J. Microsc. 156, 1. 33-40.
7. Körtje, K.H. (1994a) Image-EELS: simultaneous recording of multiple electron energy-loss spectra from series of electron spectroscopic images. J. Microsc. 174, 149-159.
8. Körtje, K.H. (1994b) Image-EELS: a synthesis of energy-loss analysis and imaging. Scanning Microsc. 8, 277-288.
9. Körtje, K.H., Paulus, U., Ibsch, M. & Rahmann, H. (1996) Imaging of thick sections of nervous tissue with energy-filtering transmission electron microscopy. J. Microsc. 183, 89-101.
10. Lavergne, J.-L., Martin, J.-M. & Belin, M. (1992) Interactive electron energy-loss elemental mapping by the 'Imaging-Spectrum' method. Microsc. Microanal. Microstruct. 3, 517-528.
11. Leapman, R.D. & Andrews, S.B. (1991) Biological electron energy loss spectroscopy: the present and the future. Microsc. Microanal. Microstruct. 2, 387-394.
12. Leapman, R.D. & Hunt, J.A. (1991) Comparison of detection limits for EELS and EDXS. Microsc. Microanal. Microstruct. 2, 231-244.
13. Mory, C., Kohl, H., Tencé, M. & Colliex, C. (1991) Experimental investigation of the ultimate EELS spatial resolution. Ultramicroscopy, 37, 191-201.
14. Müllejans, H. & Bruley, J. (1994) Improvements in detection sensitivity by spatial difference electron energy-loss spectroscopy at interfaces in ceramics. Ultramicroscopy, 53, 351-360.
15. Ottensmeyer, F.P. & Andrew, J.W. (1980) High-resolution microanalysis of biological specimens by electron energy loss spectroscopy and by electron spectroscopic imaging. J. Ultrastruct. Res. 72, 336-348.
16. Reimer, L., Fromm, I., Hirsch, P., Plate, U. & Rennekamp, R. (1992a) Combination of EELS modes and electron spectroscopic imaging and diffraction in an energy-filtering electron microscope. Ultramicroscopy, 46, 335-347.
17. Reimer, L. & Ross-Messemer, M. (1990) Contrast in the electron spectroscopic imaging mode of a TEM. II. Z-ratio, structure-sensitive and phase contrast. J. Microsc. 159, 143-160.
18. Reimer, L., Zepke, U., Moesch, J., Schulze-Hillert, S., Ross-Messemer, M., Probst, W. & Weimer, E. (1992b) EELSpectroscopy, a Reference Handbook of Standard Data for Identification and Interpretation of Electron Energy Loss Spectra and for Generation of Electron Spectroscopic Images. Carl Zeiss, Oberkochen.