Targeting of active rat α2,3-sialyltransferase to the yeast cell wall by the aid of the hsp 150Δ-carrier: Toward synthesis of sLex-decorated L-selectin ligands

Glycobiology

Volumn 6, Issue 8, 1996, Pages 851-859

  Mattila, Pirkko ^b   Joutsjoki, Vesa ^a   Kaitera, Eero ^a   Majuri, Marja Leena ^b   Niittymäki, Jaana ^b   Saris, Nina ^a   Maaheimo, Hannu ^a   Renkonen, Ossi ^c   Renkonen, Risto ^b   Makarow, Marja ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

^b UNIVERSITY OF HELSINKI   (Finland)

^c UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Secretion; Sialyl Lewis x; Sialyltransferase; Yeast

Indexed keywords

CARRIER PROTEIN; HYBRID PROTEIN; SIALYLTRANSFERASE;

ARTICLE; DRUG MANUFACTURE; ENZYME ACTIVITY; GENE CONSTRUCT; GENE EXPRESSION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PAPER CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STRUCTURE; RAT; SACCHAROMYCES CEREVISIAE;

ACETYLGLUCOSAMINE; ANIMALS; CARRIER PROTEINS; CELL WALL; HEAT-SHOCK PROTEINS; L-SELECTIN; LIGANDS; MAGNETIC RESONANCE SPECTROSCOPY; OLIGOSACCHARIDES; RATS; SACCHAROMYCES CEREVISIAE; SIALYLTRANSFERASES;

EID: 0030445221     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/6.8.851     Document Type: Article

References (49)

1. Buerke, M., Weyrich, A.S., Zheng, Z., Gaeta, F.C.A., Forrest, M.J. and Lefer, A.M. (1994) Sialyl-Lewis x-containing oligosaccharide attenuates myocardial reperfusion injury in cats. J. Clin. Invest., 93, 1140-1148.
2. 2-terminal signal anchor with a signal peptide. J. Biol. Chem. 264, 17619-17622.
3. Fast, D.G., Jamieson, J.C. and McCaffrey, G. (1993) The role of the carbohydrate chains of Galβ-1,4GlcNAc-α2,6-sialyltransferase for enzyme activity. Biochim. Biophys. Acta, 1202, 325-330.
4. Field, M.C. and Wainwright, L.J. (1995) Molecular cloning of eucaryotic glycoprotein and glycolipid glycosyltransferases: a survey. Glycobiology, 5, 463-472.
5. Gillespie, W., Kelm, S. and Paulson, J.C. (1992) Cloning and expression of the Galβ1,3GalNAcα2,3-sialyltransferase. J. Biol. Chem. 267, 21004-21010.
6. Hadfield, C., Raina, K.K., Shashi-Menon, K. and Mount, R.C. (1993) The expression and performance of cloned genes in yeast. Mycol. Res., 97, 897-944.
7. Hamamoto, T., Lee, Y.-C., Kurosawa, N., Nakaoka, T., Kojima, N. and Tsuji, S. (1994) Expression of mouse Galβ1,4GlcNAcα2,6sialyltransferase in an insoluble form in Escherichia coli and partial renaturation. Bioorg. Med. Chem., 2, 79-44.
8. Hård, K., van Zadelhoff, G., Moonen, P., Kamerling, J.P. and Vliegenthart, J.F.G. (1992) The Asn-linked carbohydrate chains of human Tamm-Horsfall glycoproteins of one male. Novel sulfated and novel N-acetylgalactosamine-containing N-linked carbohydrate chains. Eur. J. Biochem., 209, 895-915.
9. Harduin-Lepers, A., Recchi, M.-A. and Delonnoy, P. (1995) 1994, the year of the sialyltransferases. Glycobiology, 5, 741-758.
10. Haselbeck, A. and Tanner, W. (1983) O-Glycosylation in Saccaromyces cerevisiae is initiated at the endoplasmic reticulum. FEBS Lett., 158, 335-338.
11. Herrmann, G., Elling, L., Krezdorn, C.H., Kleene, R., Berger, E.G. and Wandrey, C. (1995a) Use of transformed whole yeast cells expressing β-1,4-galactosyltransferase for the synthesis of N-acetyllactosamine. Bioorg. Medicin. Chem. Lett., 5, 673-676.
12. Herrmann, G.F., Krezdorn, C., Malissard, M., Kleene, R., Paschold, H., Weuster-Botz, D., Kragl, U., Berger, E.G. and Wandrey, C. (1995b) Large-scale production of a soluble human β-1,4-galactosyltransferase using a Saccharomyces cerevisiae expression system. Protein Expression Purification, 6, 72-78.
13. Hill, J., Donald, K.A.I.G. and Griffiths, D.E. (1991) DMSO-enhanced whole cell yeast transformation Nucleic Acids Res., 19, 5791.
14. Hoffman, C.S., and Winston, F. (1987) A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene, 57, 267-272.
15. Hogg, N. and Berlin, C. (1995) Structure and function of adhesion receptors in leukocyte trafficking. Immunol. Today, 16, 327-330.
16. Holkeri, H., Simonen, M., Pummi, T., Vihinen, H. and Makarow, M. (1996) Glycosylation of rat NGF receptor ectodomain in the yeast Saccaromyces cerevisiae. FEBS Lett., 383, 255-258.
17. Ichikawa, Y., Lin, Y.-C., Dumas, D.P., Shen, G.J., Garcia-Junceda, E., Williams, M.A., Bayer, R., Ketcham, C., Walker, L., Paulson, J.C. and Wong, C.-H. (1992) Chemical-enzymatic synthesis and conformational analysis of sialyl Lewis x and derivatives. J. Am. Chem. Soc., 114, 9283-9298.
18. Jämsä, E., Simonen, M. and Makarow, M. (1994) Selective retention of secretory proteins in the yeast endoplasmic reticulum by treatment of cells with a reducing agent. Yeast, 10, 355-370.
19. Jämsä, E., Holkeri, H., Vihinen, H., Wikström, M., Simonen, M., Walse, B., Kalkkinen, N., Paakkola, J. and Makarow, M. (1995) Structural features of a polypeptide carrier promoting secretion of a β-lactamase fusion protein in yeast. Yeast, 11, 1381-1391.
20. Kaiser, C.A. and Schekman, R. (1990) Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway. Cell, 61, 723-733.
21. 1H-nuclear magnetic resonance spectroscopy of oligosaccharide alditols released from mucin-type O-glycoproteins. Biol. Magn. Res. 10, 1-287.
22. Koszdin, K. and Bowen, B. (1992) The cloning and expression of a human α1,3 fucosyltransferase capable of forming the E-selectin ligand. Biochem. Biophys. Res. Commun., 187, 152-157.
23. Krezdorn, C H., Kleene, R.B., Watzele, M., Ivanov, S.X., Hokke, C.H., Kamerling, J.P. and Berger, E.G. (1994) Human β1,4galactosyltransferase and α2-6sialyltransferase expressed in Saccharomyces cerevisiae are retained as active enzymes in the endoplasmic reticulum. Eur. J. Biochem., 220, 809-817.
24. Kukowska-Latallo, J., Larsen, R.D., Nair, R.P. and Lowe, J.B. (1990) A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the Lewis blood group α(1,3/1,4)fucosyltransferase. Genes Dev., 4, 1288-1303.
25. Kurosawa, N., Kawasaki, M., Hamamoto, T., Nakaoka, T., Lee, Y-C., Arita, M. and Tsuji, S. (1994) Molecular cloning and expression of chick embryo Galβ1,4GlcNAα2,6-sialyltransferase. Eur. J. Biochem., 219, 375-381.
26. Maaheimo, H., Renkonen, R., Turunen, J., Penttilä, L. and Renkonen, O. (1995) Synthesis of a divalent sialyl Lewis x O-glycan, a potent inhibitor of lymphocyte-endothelium adhesion. Evidence that multivalency enhances the saccharide binding to L-selectin. Eur. J. Biochem., 234, 616-625.
27. Majuri, M.-L., Pinola, M., Niemelä, R., Tiisala, S., Natunen, J., Renkonen, O. and Renkonen, R. (1994) α2,3sialyl- and α1,3fucosyltransferase-dependent synthesis of sialyl Lewis x, an essential oligosaccharide present on L-selectin counterreceptors, in cultured endothelial cells. Eur. J. Immunol , 24, 3205-3210.
28. McEver, R.P., Moore, K.L. and Cummings, R.D. (1995) Leukocyte trafficking mediated by selectin-carbohydrate interactions. J. Biol. Chem., 270, 11025-11028.
29. Mihelcic, D., Schleiffenbaum, B., Tedder, T.F., Sharar, S.R., Harlan, J.M. and Winn, R.K. (1994) Inhibition of leukocyte L-selectin function with a monoclonal antibody attenuates reperfusion injury to the rabbit ear. Blood, 84, 2322-2328.
30. Mulligan, M.S., Paulson, J.C., de Frees, S., Zheng, Z.-L., Lowe, J.B. and Ward, P.A. (1993) Protective effects of oligosaccharides in P-selectin-dependent lung injury. Nature, 364, 149-151.
31. Munro, S. (1995) An investigation of the role of transmembrane domains in Golgi retention. EMBO J., 14, 4695-4704.
32. Niemelä, R., Natunen, J., Brotherus, E., Saarikangas, A. and Renkonen, O (1995) α1,3-Fucosylation of branched blood group I-type oligo-(N-acetyllactosamino)glycans by human milk transferases is restricted to distal N-acetyllactosamine units: the resulting isomers are separated by WGA-agarose chromatography. Glycoconjugate J , 12, 36-44.
33. Pizcueta, P. and Luscinskas, F.W. (1994) Monoclonal antibody blockade of L-selectin inhibits mononuclear leukocyte recruitment to inflammatory sites in vivo. Am. J. Pathol., 145, 461-469.
34. Renkonen, O., Niemelä, R., Leppänen, A., Maaheimo, H., Seppo, A., Penttilä, L. and Vilkman, A. (1991) Construction of linear GlcNAcβ(1-6)Galβ1-OR type oligosaccharides by partial cleavage of GlcNAcβ1-3(GlcNAcβ1-6)Galβ1-OR sequences with jack bean β-N-acetylhexosaminidase. Glycoconjugate J., 8, 368-375.
35. Romanos, M.A., Scorer, C.A. and Clarke, J.J. (1992) Foreign gene expression in yeast. Yeast, 8, 423-488.
36. Rosen, S. and Bertozzi, C. (1994) The selectins and their ligands. Curr. Opin. Cell Biol., 6, 663-673.
37. Russo, P., Kalkkinen, N., Sareneva, H., Paakkola, J. and Makarow, M. (1992) A heat shock gene from Saccharomyces cerevisiae encoding a secretory glycoprotein. Proc. Natl. Acad. Sci. USA, 89, 3671-3675.
38. Russo, P., Simonen, M., Uimari, A., Teesalu, T. and Makarow, M. (1993) Dual regulation by heat and nutrient stress of the yeast HSP150 gene encoding a secretory glycoprotein. Mol. Gen. Genet., 239, 273-280.
39. Schreuder, M.P., Mooren, A.T.A., Toschka, H.Y., Verrips, C.T. and Klis, F.M. (1996) Immobilizing proteins on the surface of yeast cells. TIBTECH, 14, 115-120.
40. Schwientek, T., Lorenz, C. and Ernst, J.F. (1995) Golgi localization in yeast is mediated by the membrane anchor region of rat liver sialyltransferase. J. Biol. Chem., 270, 543-5489.
41. Seppo, A., Turunen, J.P., Penttilä, L., Keane, A., Renkonen, O. and Renkonen, R. (1996) Enzymatically synthesized oligovalent sialyl Lewis x glycans are high-affinity inhibitors of L-selectin-mediated lymphocyte binding to endothelium. Glycobiology, 6, 65-71.
42. Simonen, M., Jämsa, E. and Makarow, M. (1994) The role of the carrier protein and disulfide formation in the folding of β-lactamase fusion proteins in the endoplasmic reticulum of yeast. J. Biol. Chem., 269, 13887-13892.
43. Simonen, M , Vihinen, H., Jämsä, E., Arumäe, U., Kalkkinen, N. and Makarow, M. (1996) The hsp 150Δ-carrier confers secretion competence to the rat nerve growth factor receptor ectodomain in Saccaromyces cerevisiae. Yeast, 12, 457-466.
44. Turunen, J-P., Paavonen, T., Majuri, M.-L., Tiisala, S., Mattila, P., Mennander, A., Gahmberg, C., Hayry, P., Tamatani, T., Miyasaka, M. and Renkonen, R. (1994) Sialyl Lewis x and L-selectin-dependent site-specific lymphocyte extravasation into renal transplants during acute rejection. Eur. J. Immunol 24, 1130-1136.
45. Turunen, J., Majuri, M., Seppo, A., Tiisala, S., Paavonen, T., Miyasaka, M., Lemström, K , Penttilä, L., Renkonen, O. and Renkonen, R. (1995) De novo expression of endothelial sialyl Lewis a and sialyl Lewis x during cardiac transplant rejection. Superior capacity of a tetravalent sLex-oligosaccharide in inhibiting L-selectin-dependent lymphocyte adhesion. J. Exp. Med., 182, 1133-1142.
46. Weinstein, J., de Souza-e-Silva, U. and Paulson, J.C. (1982) Purification of a Galβ1-GlcNAcα2-6sialyltransferase and a Galβ1-3(4)GlcNAcα2-3sialyltransferase to homogeneity from rat liver. J. Biol. Chem., 257, 13835-13844.
47. Wen, D.X., Livingston, B.D., Medzihradszky, K.F., Kelm, S., Burlinggame, A.L. and Paulson, J.C. (1992) Primary structure of Galβ1,3(4)GlcNAcα-2,3sialyltransferase determined by mass spectrometry sequence analysis and molecular cloning. J. Biol. Chem., 267, 21011-21019.
48. Whelan, J. (1996) Selectin synthesis and inflammation. Trends Biol. Sci., 21, 65-69.
49. Williams, M.A., Kitagawa, H., Datta, A.K., Paulson, J.C. and Jamieson, J.C. (1995) Large-scale expression of recombinant sialyltransferases and comparison of their kinetic properties with native enzymes. Glycoconjugate J., 12, 755-761