MHP1, an essential gene in Saccharomyces cerevisiae required for microtubule function

Journal of Cell Biology

Volumn 135, Issue 5, 1996, Pages 1323-1339

  Irminger Finger, Irmgard ^a   Hurt, Ed ^b   Roebuck, Aline ^a   Collart, Martine A ^a   Edelstein, Stuart J ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MICROTUBULE ASSOCIATED PROTEIN;

ARTICLE; GENE DISRUPTION; GENE EXPRESSION REGULATION; GENE ISOLATION; MICROTUBULE ASSEMBLY; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN STABILITY; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; BLOTTING, WESTERN; CELL DIVISION; CLONING, MOLECULAR; EPITOPE MAPPING; FUNGAL PROTEINS; GENE DELETION; GENE EXPRESSION; GENES, FUNGAL; GENETIC COMPLEMENTATION TEST; IMMUNE SERA; INTERPHASE; ISOELECTRIC POINT; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; MITOSIS; MOLECULAR SEQUENCE DATA; PHENOTYPE; PHOSPHORYLATION; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ANALYSIS;

EID: 0030444680     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.135.5.1323     Document Type: Article

References (70)

1. Aizawa, H., H. Kawasaki, H. Murofushi, S. Kotani, K. Suzuki, and H. Sakai. 1989. A common amino acid sequence in 190-kDa microtubule-associated protein and tau for the promotion of microtubule assembly. J. Biol. Chem. 264:5885-5890.
2. r 190,000. J Biol. Chem. 265:13849-13855.
3. Aizawa, H., M. Kamijo, Y. Ohba, A. Mori, K. Okuhara, H. Kawasaki, H. Murofushi, K. Suzuki, and H. Yasuda. 1991. Microtubule destabilization by cdcd2/H1 histone kinase: phosphorylation of a "Pro-rich region" in the microtubule-binding domain of MAP4. Biochem. Biophys. Res. Commun. 179:1620-1626.
4. Barnes, G., A. Louie, and D. Botstein. 1992. Yeast proteins associated with microtubules in vitro and in vivo. Mol. Biol. Cell. 3:29-47.
5. Bellocq, C., I. Andrey-Tornare, A.-M. Paunier, B. Maeder, L. Paturle, D. Job, J. Haiech, and S. Edelstein. 1992. Purification of assembly-competent tubulin from Saccharomyces cerevisiae. Eur. J. Biochem. 210:343-349.
6. Berlin, V., C.A. Styles, and G.R. Fink. 1990. B1K1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin. J. Cell Biol. 111:2573-2586.
7. Botstein, D., S.C. Falco, S.E. Stewart, M. Brennan, S. Scherer, D.T. Stinchcomb, K. Struhl, and R.W. Davis. 1979. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene (Amst.). 8:17-24.
8. Bulinski, J.C., and G.G. Borisy. 1979. Self-assembly of microtubules in extracts of cultured HeLa cells and the identification of HeLa microtubule-associated protein. Proc. Natl. Acad. Sci. USA. 76:293-297.
9. Bulinski, J.C., and G.G. Borisy. 1980. Widespread distribution of a 210,000 mol wt microtubule-associated protein in cells and tissues of primates. J. Cell Biol. 87:802-808.
10. r microtubule-associated protein (MAP4) contains a domain homologous to the microtubule-binding domains of neural MAP2 and tau. J. Cell Sci. 98:27-36.
11. Chou, P., and G.D. Fasman. 1978. Prediction of the secondary structure of proteins from their amino acid sequence. Adv. Enzymol. 47:45-147.
12. Cleveland, D.W., S.Y. Hwo, and M.W. Kirschner. 1977. Physical and chemical properties of purified tau factor and the role of tau in microtubule assembly. J. Mol. Biol. 116:227-247.
13. Colicelli, J. C. Birchmeier, T. Michaeli, K. O'Neill, M. Riggs, and M. Wigler. 1989. Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase. Proc. Natl. Acad. Sci. USA. 86:3599-3603.
14. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
15. Diaz-Nido, J., L. Serrano, E. Mendez, and J. Avila. 1988. A casein kinase II-related activity is involved in phosphorylation of microtubule-associated protein MAPI B during neuroblastoma cell differentiation. J. Cell Biol. 106:2057-2065.
16. Edelmann, A.M., D.K. Blumenthal, and E.G. Krebs. 1987. Protein serine/threonine kinases. Annu. Rev. Biochem. 56:567-613.
17. Gamier, J., D.J. Osguthorpe, and B. Robson. 1978. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J. Mol. Biol. 120:97-120.
18. Goldstein, L.S.B., R.A. Laymon, and J.R. McIntosh. 1986. A microtubule-associated protein in Drosophila melanogaster. identification, characterization, and isolation of coding sequences. J. Cell Biol. 107:2076-2087.
19. Gonzales, G.A., K.K. Yamamoto, W.H. Fischer, D. Karr, P. Menzel, I. Biggs, W.W. Vale, and M.R. Montminy. 1989. A cluster of phosphorylation sites on the cyclic AMP-regulated nuclear factor CRBE predicted by its sequence. Nature (Lond.). 337:749-752.
20. Goode, B.L., and S.C. Feinstein. 1994. Identification of a novel microtubule binding and assembly domain in the developmentally regulated inter-repeat region of tau. J. Cell Biol. 124:769-782.
21. Hasek, J., J. Jochova, P. Draber, V. Viklicky, and E. Streiblova. 1991. Localization of a 210-kD microtubule-interacting protein in the yeast Saccharomyces cerevisiae. Can. J. Microbiol. 38:149-152.
22. Hemphill, A., M. Affolter, and T. Seebeck. 1992. A novel microtubule-binding motif identified in a high molecular weight microtubule-associated protein from Trypanosoma brucei. J. Cell Biol. 117:95-103.
23. Hoyt, M.A., T. Sterns, and D. Botstein. 1990. Chromosome instability mutants of Saccharomyces cerevisiae that are defective in microtubule-mediated processes. Mol. Cell. Biol. 10:223-234.
24. Hoyt, A.M., L. He, K.K. Loo, and W.S. Saunders. 1992. Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly. J. Cell Biol. 118:109-120.
25. Hurt, E.C. 1988. A novel nucleoskeletal-like protein located at the nuclear periphery is required for the life cycle of the yeast Saccharomyces cerevisiae. EMBO (Eur. Mol. Biol. Organ.) J. 7:4323-4334.
26. Hyman, A.A., K. Middleton, M. Centola, T.J. Mitchison, and J. Carbon. 1992. Microtubule-motor activity of a yeast centromere-binding protein complex. Nature (Lond.). 359:533-539.
27. Interthal, H., C. Bellocq, J. Bahler, V.I. Bashkirov, S. Edelstein, and W.D. Heyer. 1995. A role of Sep1 (=Kem1, Xrn1) as a microtubule-associated protein in Saccharomyces cerevisiae. EMBO (Eur. Mol. Biol. Organ.) J. 14:1057-1066.
28. Irminger-Finger, I., R.A. Laymon, and L.S.B. Goldstein. 1990. Analysis of the primary sequence and microtubule-binding region of the Drosophila 205K MAP. J. Cell Biol. 111:2563-2572.
29. Izant, J., J. Weatherbee, and J.R. McIntosh. 1983. A microtubule-associated protein antigen unique to mitotic spindle microtubules in PtK1 cells. J. Cell Biol. 96:424-434.
30. Jiang, W., K. Middleton, H. Yoon, C. Fouquet, and J. Carbon. 1993. An essential yeast protein, CBF5p, binds in vitro to centromeres and microtubules. Mol. Cell. Biol. 13:4884-4893.
31. Kilmartin, J.V., and A.E. Adams. 1984. Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces. J. Cell Biol. 98:922-933.
32. Kilmartin, J.V., B. Wright, and C. Milstein. 1982. Rat monoclonal anti-tubulin antibodies derived by using a new nonsecreting rat cell line. J. Cell Biol. 93:576-582.
33. Kilmartin, J.V., S.L. Dyos, D. Kershaw, and J.T. Finch. 1993. A spacer protein in the Saccharomyces cerevisiae spindle pole body whose transcript is cell cycle-regulated. J. Cell Biol. 123:1175-1184.
34. Kimble, M., A.L. Khodjakov, and R. Kuriyama. 1992. Identification of ubiquitous high-molecular-mass, heat-stable microtubule-associated proteins (MAPs) that are related to the Drosophila 205-kDa MAP but are not related to the mammalian MAP4. Proc. Natl. Acad. Sci. USA. 89:7693-7697.
35. Lee, G. 1993. Non-motor microtubule-associated proteins. Curr. Opin. Cell Biol. 5:88-94.
36. Lee, G., N. Cowan, and M. Kirschner. 1988. The primary structure and heterogeneity of tau protein from mouse brain. Science (Wash. DC). 239:265-288.
37. Lewis, S.A., D. Wang, and N.J. Cowan. 1988. Microtubule-associated protein MAP2 shares a microtubule-binding motif with tau protein. Science (Wash. DC). 242:936-939.
38. Li, Y.-Y., E. Yeh, and K. Bloom. 1993. Disruption of mitotic spindle orientation in a yeast dynein mutant. Proc. Natl. Acad. Sci. USA. 90:10096-10100.
39. Lillie, S.H., and S.S. Brown. 1992. Suppression of a myosin defect by a kinesin-related gene. Nature (Lond.). 356:358-361.
40. Maniatis, T., E.F. Fritsch, and J. Sambrook. 1989. Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 545 pp.
41. McMillan, J.N., and K. Tatchell. 1994. The JNM1 gene in the yeast Saccharomyces cerevisiae is required for nuclear migration and spindle orientation during the mitotic cell cycle. J. Cell Biol. 125:143-158.
42. Meluh, P.B., and M.D. Rose. 1990. KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell. 60:1029-1041.
43. Munro, S., and H.R.B. Pelham. 1984. Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila hsp70. EMBO (Eur. Mol. Biol. Organ.) J. 3:3087-3093.
44. Murphy, D.B., R.B. Vallee, and G.G. Borisy. 1977. Identity and polymerization-stimulatory activity of the non-tubulin proteins associated with microtubules. Biochemistry. 16:2598-2605.
45. Noble, M., S.A. Lewis, and N.J. Cowan. 1989. The microtubule-binding domain of microtubule-associated protein MAP1B contains a repeated sequence motif unrelated to that of MAP2 and tau. J. Cell Biol. 109:3367-3376.
46. Olmsted, J.B. 1986. Analysis of cytoskeletal structures using blot-purified monospecific antibodies. Methods Enzymol. 134:467-472.
47. Olson, K.R., J.R. McIntosh, and J.B. Olmsted. 1995. Analysis of MAP4 function in living cells using green fluorescent protein (GFP) chimeras. J. Cell Biol. 130:639-650.
48. Ookata, K., S. Hisanaga, J.C. Bulinski, H. Murofushi, H. Aizawa. T.J. Itoh, H. Hotani, E. Okumura, K. Tachibana, and T. Kishimoto. 1995. Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics. J. Cell Biol. 128:849-862.
49. Ortega Perez, R., I. Irminger-Finger, J.F. Arrighi, N. Capelli, D. van Tuinen, and G. Turian. 1994. Identification and partial purification of calmodulin-binding microtubule-associated proteins from Neurospora crassa. Eur. J. Biochem. 226:303-310.
50. Pasqualone, D., and T.C. Huffaker. 1994. STU1, a suppressor of a β-tubulin mutation, encodes a novel and essential component of the yeast mitotic spindle. J. Cell Biol. 127:1973-1984.
51. Pearson, R.B., J.R. Woodgett, P. Cohen, and B.E. Kemp. 1985. Substrate specificity of a multifunctional calmodulin-dependent protein kinase. J. Biol. Chem. 260:14471-14476.
52. Pearson, W.R., and D.J. Lipman. 1988. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA. 85:2444-2448.
53. Pereira, A., J. Doshen, E. Tanaka, and L.S.B. Goldstein. 1992. Genetic analysis of a Drosophila microtubule-associated protein. J. Cell Biol. 116:377-383.
54. Roberts, B. 1989. Nuclear location signal-mediated protein transport. Biochem. Biophys. Acta. 1008:263-280.
55. Roof, D.M., P.M. Meluh, and M.D. Rose. 1992. Kinesin-related proteins required for assembly of the mitotic spindle. J. Cell Biol. 118:95-108.
56. Rose, M.D., F. Winston, and P. Hierter. 1990. Methods in Yeast Genetics. A Laboratory Course Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 198 pp.
57. Rothstein, R.J. 1983. One step gene disruption in yeast. Methods Enzymol. 101:202-210.
58. Sanger, F., S. Michlen, and A.R. Coulson. 1977. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74:5463-5467.
59. Schoenfeld, T.A., L. McKerracher, R. Obar, and R.B. Vallee. 1989. MAP 1A and MAP 1B are structurally related microtubule-associated proteins with distinct developmental patterns in the CNS. J. Neurosci. 9:1712-1730.
60. Sherman, F., G.R. Fink, and J.B. Hicks. 1986. Methods in Yeast Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 163-167.
61. Sikorski, R.S., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 122:19-27.
62. Stearns, T., A. Hoyt, and D. Botstein. 1990. Yeast mutants sensitive to antimicrotubule drugs define three genes that regulate microtubule function. Genetics. 124:251-262.
63. Strohm, M., P. Vollmer, T.J. Tan, and D. Gallwitz. 1993. A yeast GTPase-activating protein that interacts specifically with a member of the YPT/RAB family. Nature (Lond.). 361:736-739.
64. Studier, W., A.H. Rosenberg, J.J. Dunn, and J.W. Dubendorff. 1990. Use of the T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89.
65. Vallee, R.B. 1982. A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs). J. Cell Biol. 92:435-442.
66. Vallen, E.A., W. Ho, M. Winey, and M.D. Rose. 1994. Genetic interactions between CDC31 and KAR1, two genes required for duplication of the microtubule organizing center in Saccharomyces cerevisiae. Genetics. 137:407-422.
67. Vandré, D.D., V.E. Centonze, J. Peloquin, R.M. Tombes, and G.G. Borisy. 1991. Proteins of the mammalian mitotic spindle: phosphorylation/dephosphorylation of MAP-4 during mitosis. J. Cell Sci. 98:577-588.
68. Wach, A., A. Brachat, R. Pöhlmann, and P. Philippsen. 1994. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast. 10:1793-1808.
69. West, R.R., K.M. Tenbarge, and J.B. Olmsted. 1991. A model for microtubule-associated protein 4 structure. J. Biol. Chem. 266:21886-21896.
70. Wiche, G., C. Oberkanins, and A. Himmler. 1991. Molecular structure and function of microtubule-associated proteins. Int. Rev. Cytol. 124:217-273.